Agrobacterium tumefaciens-mediated transformation and transgenic-plant regeneration of onion (Allium cepa L.).

abstract：:Med8, a subunit of mediator complex, has proved to possess crucial functions in many organisms from yeast to human. In plant, the med8 mutant of Arabidopsis thaliana displayed delayed anthesis and increased number of leaves during the vegetative period. However, the roles of Med8 in other flowering plants are still un...

journal_title：Plant cell reports

authors： Wang F,Wei H,Tong Z,Zhang X,Yang Z,Lan T,Duan Y,Wu W

更新日期：2011-11-01 00:00:00

abstract：:Donor plants of Hordeum vulgare L. cv. Igri were grown in a conditioned environment to minimise fluctuations in the composition of the microspore population. After isolation different types of microspores were identified within each population, amongst others an embryogenic subpopulation. It was shown that the optimum...

journal_title：Plant cell reports

authors： Hoekstra S,van Zijderveld MH,Heidekamp F,van der Mark F

更新日期：1993-10-01 00:00:00

abstract：:A series of RAPD markers generated by a single 10-mer primer were analyzed by hybridization to amplified and genomic DNA and by sequencing in two Brassica species. Primer B18 produced different profiles of nine major bands each in both Brassica nigra (B genome) and B. napus (AC genomes). Cloning and sequencing of five...

journal_title：Plant cell reports

authors： Quiros CF,This P,Laudie M,Benet A,Chevre AM,Delseny M

更新日期：1995-07-01 00:00:00

abstract：:From the in vitro hairy root cultures of a Datura candida hybrid, 19 tropane alkaloids have been identified using capillary gas-liquid chromatography and mass spectrometry. As in the parent plants, scopolamine is the major alkaloid. Two hitherto undescribed alkaloids have been detected and their structure tentatively ...

journal_title：Plant cell reports

authors： Christen P,Roberts MF,David Phillipson J,Evans WC

更新日期：1990-07-01 00:00:00

abstract：:RNA interference (RNAi) is a sequence-specific, posttranscriptional gene silencing (PTGS) process in plants that is mediated by dsRNA homologous to the silenced gene(s). In this study, we report an efficient method to produce dsRNA using a bacterial expression system. Two fragments of the Sugarcane Mosaic Virus (SCMV)...

journal_title：Plant cell reports

authors： Gan D,Zhang J,Jiang H,Jiang T,Zhu S,Cheng B

更新日期：2010-11-01 00:00:00

abstract：:Grapefruit (Citrus paradisi) tissue cultures were examined for qualitative and quantitative changes in flavanone-neohesperidoside content during somatic embryogenesis. Embryos cultured in vitro contain naringin and a rhamnosyl-transferase activity which is capable of rhamnosylating position 2 on the flavanone glucosid...

journal_title：Plant cell reports

authors： Gavish H,Lewinsohn E,Vardi A,Fluhr R

更新日期：1989-10-01 00:00:00

abstract：:Eukaryotic gene expression is regulated at least by two processes, RNA interference at the post-transcriptional level and chromatin modification at the transcriptional level. Distinct small RNAs (approximately 21-24 nucleotides; sRNAs) were demonstrated to play vital roles in facilitating gene silencing. In plants, th...

journal_title：Plant cell reports

authors： Qian Y,Cheng Y,Cheng X,Jiang H,Zhu S,Cheng B

更新日期：2011-07-01 00:00:00

abstract：:Leaf explants ofKalanchoe laciniata were cocultivated for different days (2, 4, 6 and 8 days) with disarmedAgrobacterium tumefaciens strains A208SE, GV3111SE and EHA101 carring a binary vector pROA93. The vector contains a cauliflower mosaic virus 35S promotor which drives the coding sequence of neomycin phosphotransf...

journal_title：Plant cell reports

authors： Jia SR,Yang MZ,Ott R,Chua NH

更新日期：1989-06-01 00:00:00

abstract：:This article describes conditions to optimize the yield of viable protoplasts from callus tissue of Asparagus densiflorus cv. Sprengeri and their subsequent regeneration into plantlets. Callus tissue was initiated by culturing spear sections (5-7 mm) on Murashige and Skoog (MS) medium supplemented with 0.8% (wt/vol) B...

journal_title：Plant cell reports

authors： Benmoussa M,Mukhopadhyay S,Desjardins Y

更新日期：1997-12-01 00:00:00

abstract：: A high frequency of embryogenesis and transformation from all parts of flowers of two lines of Medicago truncatula R-108-1 and Jemalong J5 were obtained. Using this flower system, we obtained transgenic plants expressing promoter-uidA gene fusions as well as the gfp living cell color reporter gene. Moreover, this met...

journal_title：Plant cell reports

authors： Kamaté K,Rodriguez-Llorente ID,Scholte M,Durand P,Ratet P,Kondorosi E,Kondorosi A,Trinh TH

更新日期：2000-06-01 00:00:00

abstract：:Transgenic herbicide tolerant Acacia sinuata plants were produced by transformation with the bar gene conferring phosphinothricin resistance. Precultured hypocotyl explants were infected with Agrobacterium tumefaciens strain EHA105 in the presence of 100 microM acetosyringone and shoots regenerated on MS (Murashige an...

journal_title：Plant cell reports

authors： Vengadesan G,Amutha S,Muruganantham M,Anand RP,Ganapathi A

更新日期：2006-11-01 00:00:00

abstract：:Somatic embryogenesis was induced in callus tissues derived from young flower buds ofPanax notoginseng via callus within 18 weeks of culture. The mature somatic embryos were germinated on half-strength Murashige and Skoog's (MS) medium supplemented with gibberellic acid A3(GA) and 6-benzyladenine (BA). The most suitab...

journal_title：Plant cell reports

authors： Shoyama Y,Zhu XX,Nakai R,Shiraishi S,Kohda H

更新日期：1997-04-01 00:00:00

abstract：:Brassinosteroids are known to promote cell elongation in a wide range of plant species but their effect on cell division has not been as extensively studied. We examined the effect of brassinolide on the kinetics and final division frequencies of regenerating leaf mesophyll protoplasts of Petunia hybrida Vilm v. Coman...

journal_title：Plant cell reports

authors： Oh MH,Clouse SD

更新日期：1998-09-01 00:00:00

abstract：KEY MESSAGE:In plants, phosphorylated MAPKs display constitutive nuclear localization; however, not all studied plant species show co-localization of activated MAPKs to mitotic microtubules. The mitogen-activated protein kinase (MAPK) signaling pathway is involved not only in the cellular response to biotic and abiotic...

journal_title：Plant cell reports

authors： Winnicki K,Żabka A,Bernasińska J,Matczak K,Maszewski J

更新日期：2015-06-01 00:00:00

abstract：:A protocol for whole plant regeneration of Cicer arietinum L. cv. C-235 via organogenesis from callus has been developed. Callus initiation was best when immature leaflets were cultured on MS medium containing 5 or 25 μM 2,4-D or NAA in combination with 10 μM BA, or 25 μM 2,4-D alone. The callus grew most vigorously o...

journal_title：Plant cell reports

authors： Barna KS,Wakhlu AK

更新日期：1994-06-01 00:00:00

abstract：: Cells of a suspension culture of Doritaenopsis cv. New Toyohashi were placed in a mixture of 2 M glycerol and 0.4 M sucrose for 15 min at room temperature and then dehydrated with a vitrification solution (PVS2) for 1-3 h on ice and plunged into liquid nitrogen. The highest viability (64% by 2,3,5-triphenyltetrazoliu...

journal_title：Plant cell reports

authors： Tsukazaki H,Mii M,Tokuhara K,Ishikawa K

更新日期：2000-12-01 00:00:00

abstract：:Murashige and Skoog's modified medium in 1% Difco Bacto-agar supplemented with sugar alcohols (sorbsitol, mannitol), growth regulators (1-naphthalenacetic acid, 2,4-dichlorophenoxyacetic acid, benzyladenine, kinetin) and sugars (fructose, glucose, sucrose) induced aposporous gametophytes from pinnae of Pteris vittata ...

journal_title：Plant cell reports

authors： Kwa SH,Wee YC,Loh CS

更新日期：1991-10-01 00:00:00

abstract：:Globe artichoke represents a natural source of phenolic compounds with dicaffeoylquinic acids along with their biosynthetic precursor chlorogenic acid (5-caffeoylquinic acid) as the predominant molecules. We report the isolation and characterization of a full-length cDNA and promoter of a globe artichoke p-coumaroyl e...

journal_title：Plant cell reports

authors： Moglia A,Comino C,Portis E,Acquadro A,De Vos RC,Beekwilder J,Lanteri S

更新日期：2009-06-01 00:00:00

abstract：:In leek (Allium ampeloprasum L.) a cyclic system of somatic embryogenesis was developed. Somatic embryos used for cyclic embryogenesis were able to develop the same type of embryogenic callus as zygotic embryos in the primary cycle. For the first time a comparison of the efficiencies of both expiants was made. Ten fam...

journal_title：Plant cell reports

authors： Schavemaker CM,Jacobsen E

更新日期：1995-01-01 00:00:00

abstract：KEY MESSAGE:A plant-derived 0.3 kb constitutive promoter was obtained from AtTCTP expression analysis, and successfully applied to the expression of a selectable marker gene for production of transgenic creeping bentgrass plants. The isolation and use of an efficient promoter is essential to develop a vector system for...

journal_title：Plant cell reports

authors： Han YJ,Kim YM,Hwang OJ,Kim JI

更新日期：2015-02-01 00:00:00

abstract：:Suspension cultures derived from Lycium chinense Miller seedlings produced significant amounts of a hepatoprotective cerebroside. Callus was induced from the stem of aseptic seedlings of L. chinense and maintained on MS solid media supplemented with 1.0 ppm 2,4-D and 0.1 ppm kinetin. Suspension cultures were establish...

journal_title：Plant cell reports

authors： Jang YP,Lee YJ,Kim YC,Huh H

更新日期：1998-12-01 00:00:00

abstract：KEY MESSAGE:The gene and cDNA sequence encoding PAL from Chinese medicinal plant Rhus chinensis were cloned and analyzed, furthermore the biochemical properties, kinetic parameters, differential expression and key sites were studied. Rhus chinensis is a well-known Chinese medicinal plant. Phenylalanine ammonia-lyase (P...

journal_title：Plant cell reports

authors： Ma W,Wu M,Wu Y,Ren Z,Zhong Y

更新日期：2013-08-01 00:00:00

abstract：:Citrus psorosis is a serious viral disease affecting citrus trees in many countries. Its causal agent is Citrus psorosis virus (CPsV), the type member of genus Ophiovirus. CPsV infects most important citrus varieties, including oranges, mandarins and grapefruits, as well as hybrids and citrus relatives used as rootsto...

journal_title：Plant cell reports

authors： Zanek MC,Reyes CA,Cervera M,Peña EJ,Velázquez K,Costa N,Plata MI,Grau O,Peña L,García ML

更新日期：2008-01-01 00:00:00

abstract：:Genistein, 4',5,7-trihydroxyisoflavone, is an isoflavonoid compound predominantly restricted to legumes and known to possess phyto-oestrogenic and antioxidative activities. The key enzyme that redirects phenylpropanoid pathway intermediates from flavonoids to isoflavonoids is the isoflavone synthase (IFS). Brassica na...

journal_title：Plant cell reports

authors： Li X,Qin JC,Wang QY,Wu X,Lang CY,Pan HY,Gruber MY,Gao MJ

更新日期：2011-08-01 00:00:00

abstract：:Somatic hybrids were obtained by the symmetric fusion of embryogenic callus cells from tetraploid 'Mame' kumquat [Fortunella hindsii (Champ.) Swing.] and mesophyll cells from diploid trifoliate orange [Poncirus trifoliata (L.) Raf.]. Southern blot analysis of three regenerants revealed that they carried specific rDNA ...

journal_title：Plant cell reports

authors： Miranda M,Motomura T,Ikeda F,Ohgawara T,Saito W,Endo T,Omura M,Moriguchi T

更新日期：1997-03-01 00:00:00

abstract：:Human growth hormone (hGH), a pituitary-derived polypeptide, evidences a wide range of biological functions, including protein synthesis, cell proliferation, and metabolism. A synthetic hGH gene (shGH) has been synthesized on the basis of plant-optimized codon usage via an overlap PCR strategy and located in a plant e...

journal_title：Plant cell reports

authors： Kim TG,Baek MY,Lee EK,Kwon TH,Yang MS

更新日期：2008-05-01 00:00:00

abstract：:An efficient plant regeneration system from leaf-derived callus in 6 genotypes of Avena sativa L. has been established. Regenerable callus was induced in the basal 1-2 mm region of 2 to 5 day old seedlings. Plants were regenerated from the regenerable callus and grown to maturity. The frequency of regenerable callus f...

journal_title：Plant cell reports

authors： Chen H,Xu G,Loschke DC,Tomaska L,Rolfe BG

更新日期：1995-03-01 00:00:00

abstract：:Somatic embroys and subsequent plant regeneration were obtained from isolated leaf epidermis of Gaillardia picta. Abaxial and adaxial epidermal peels (monolayer) from 45 days old aseptic seedlings were isolated and segments measuring 5 mm x 3 mm were cultured on B5 basal medium supplemented with various growth regulat...

journal_title：Plant cell reports

authors： Pillai KG,Rao IU,Rao IV,Ram HY

更新日期：1992-01-01 00:00:00

abstract：:The sugarcane (Saccharum officinarum) stress-related MYB transcription factor gene, ScMYBAS1, demonstrated induced response to water deficit and salt stress in our previous study. To elucidate its stress tolerance mechanism at the transcriptional level, we isolated and characterized the promoter (PScMYBAS1, 1,033 bp) ...

journal_title：Plant cell reports

authors： Prabu G,Prasad DT

更新日期：2012-04-01 00:00:00

abstract：KEY MESSAGE:Marker-free transgenic plants can be generated with high efficiency by using the Cre/ lox P self-excision system controlled by the pollen- and embryo-specific Arabidopsis DLL promoter. In this work, we aimed to study the feasibility of using the pollen- and embryo-specific DLL promoter of the At4g16160 gene...

journal_title：Plant cell reports

authors： Polóniová Z,Jopčík M,Matušíková I,Libantová J,Moravčíková J

更新日期：2015-03-01 00:00:00