Abstract：
:A new method for obtaining transgenic cowpea was developed using positive selection based on the Escherichia coli 6-phosphomannose isomerase gene as the selectable marker and mannose as the selective agent. Only transformed cells were capable of utilizing mannose as a carbon source. Cotyledonary node explants from 4-day-old in vitro-germinated seedlings of cultivar Pusa Komal were inoculated with Agrobacterium tumefaciens strain EHA105 carrying the vector pNOV2819. Regenerating transformed shoots were selected on medium supplemented with a combination of 20 g/l mannose and 5 g/l sucrose as carbon source. The transformed shoots were rooted on medium devoid of mannose. Transformation efficiency based on PCR analysis of individual putative transformed shoots was 3.6%. Southern blot analysis on five randomly chosen PCR-positive plants confirmed the integration of the pmi transgene. Qualitative reverse transcription (qRT-PCR) analysis demonstrated the expression of pmi in T₀ transgenic plants. Chlorophenol red (CPR) assays confirmed the activity of PMI in transgenic plants, and the gene was transmitted to progeny in a Mendelian fashion. The transformation method presented here for cowpea using mannose selection is efficient and reproducible, and could be used to introduce a desirable gene(s) into cowpea for biotic and abiotic stress tolerance.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Bakshi S,Saha B,Roy NK,Mishra S,Panda SK,Sahoo Ldoi
10.1007/s00299-012-1230-3subject
Has Abstractpub_date
2012-06-01 00:00:00pages
1093-103issue
6eissn
0721-7714issn
1432-203Xjournal_volume
31pub_type
杂志文章abstract：:Ginger (Zingiber officinale Rosc.) is prone to photoinhibition under intense sunlight. Excessive light can be dissipated by the xanthophyll cycle, where violaxanthin de-epoxidase (VDE) plays a critical role in protecting the photosynthesis apparatus from the damage of excessive light. We isolated ~2.0 kb of ginger VDE...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-012-1254-8
更新日期：2012-08-01 00:00:00
abstract：:Transgenic calli of Pharbitis nil which grow in the presence of kanamycin were obtained by introduction of plasmid pBI121, which carries kanamycin resistance and the gene for beta-glucuronidase. The calli were shown to have fragments of vector DNA in their genome and high levels of beta-glucuronidase activity. This is...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00274124
更新日期：1989-05-01 00:00:00
abstract：:In a study of the 5'-flanking sequence of the Zea mays L. (maize) Glb1 gene in vitro, serial promoter deletions were generated and linked with the β-glucuronidase (GUS) reporter gene. The promoter deletion-GUS fusions were introduced into the maize P3377 cell line by particle bombardment. GUS assays indicated that tre...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990050459
更新日期：1998-05-01 00:00:00
abstract：:In vitro plant regeneration via embryogenesis was obtained in suspension cultures of Datura innoxia Mill. Embryogenesis was induced in suspension cultures raised from callus of androgenetic origin, using LS liquid medium supplemented with 0.22 mg/l 2,4-D. The total number of embryos formed was variable over time in cu...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00233065
更新日期：1993-08-01 00:00:00
abstract：:Phytohormones are essential signal compounds in the regulation of stress-related and defense-related genes. However, there is no clear evidence for any effect of these signal molecules and biotic elicitors on the regulation of the SALT gene in suspension-cultured rice cells. We characterized the expression of a SALT g...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-004-0836-5
更新日期：2004-10-01 00:00:00
abstract：:Cryopreservation has been shown to improve the frequency of virus elimination - specifically cucumber mosaic virus and banana streak virus - from banana ( Musa spp.) plants. To understand the mode of action of cryopreservation for the eradication of viral particles, we examined the ultrastructure of meristem tips at e...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-002-0537-x
更新日期：2003-03-01 00:00:00
abstract：:Cell suspension cultures of tomato (Lycopersicon esculentum) adapted to growing continuously in the presence of 0.1 mM CdCl2 and accumulated phytochelatins (PCs, poly(γ-Glu-Cys)n-Gly). The highest level of PCs was measured 4 days after inoculation and coincided with the peak of cellular cadmium concentration. At this ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232275
更新日期：1990-12-01 00:00:00
abstract：KEY MESSAGE:Drastic changes in soil water content can activate the short-term high expression of key enzyme-encoding genes involved in secondary metabolite synthesis thereby increasing the content of secondary metabolites. Bupleurum chinense DC. is a traditional medicinal herb that is famous for its abundant saikosapon...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-019-02436-8
更新日期：2019-09-01 00:00:00
abstract：:Embryogenic cell suspension cultures of garlic (Allium sativum L.) were initiated in liquid medium from friable embryogenic tissue. The optimal parameters for culture maintenance were: (1) an initial cell density of 1-4% (v/v); (2) medium renewal every 14 days and subculturing every 28 days; (3) a low 2,4-dichlorophen...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-005-0937-9
更新日期：2005-08-01 00:00:00
abstract：:Somatic embryos and plantlets have been obtained in quite a direct way from mesophyll protoplasts isolated from androgenetic plants of two cultivars ("Loras" and "Tower") of Brassica napus. The procedure consists of four steps. Proembryos were induced in a medium supplemented with a cytokinin and an auxin at comparati...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00270237
更新日期：1982-10-01 00:00:00
abstract：:Jasmonates enhance the expression of various genes involved in terpenoid indole alkaloid (TIA) biosynthesis in Catharanthus roseus. We applied precursor feeding to our C. roseus suspensions to determine how methyl jasmonate (MJ) alters the precursor availability for TIA biosynthesis. C. roseus suspensions were induced...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-005-0109-y
更新日期：2006-06-01 00:00:00
abstract：:Conditions favourable to embryogenesis from isolated microspores of Brassica rapa L. ssp. oleifera (canola quality) were identified. A population with enhanced responsiveness for microspore embryogenesis (C200) was synthesized by crossing individual plants showing microspore embryogenic potential. For optimal microspo...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232537
更新日期：1992-05-01 00:00:00
abstract：KEY MESSAGE:Cowpea miRNAs and Argonaute genes showed differential expression patterns in response to CPSMV challenge Several biotic stresses affect cowpea production and yield. CPSMV stands out for causing severe negative impacts on cowpea. Plants have two main induced immune systems. In the basal system (PTI, PAMP-tri...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-020-02548-6
更新日期：2020-08-01 00:00:00
abstract：:The first enzyme in the flavonoid pathway, chalcone synthase, is encoded by a gene (CHS) whose expression is normally under developmental control. In our previous studies, an 896-bp promoter region of a flower-specific CHS gene was isolated from Lilium orential 'Sorbonne', and designated as PLoCHS. Here, the PLoCHS pr...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-011-1124-9
更新日期：2011-12-01 00:00:00
abstract：:The transit peptide sequence of ibAGP2 (TP2) was found to be capable of targeting protein into the chloroplast in the Arabidopsis protoplasts. TP2 was fused to a beta-glucuronidase (GUS) reporter gene and expressed in Arabidopsis under the control of the ibAGP2 promoter with the aim of dissecting the effect of the tra...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-008-0563-4
更新日期：2008-08-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232946
更新日期：1994-06-01 00:00:00
abstract：:Emission and plant uptake of atmospheric nitrogen oxides (NO + NO(2)) significantly influence regional climate change by regulating the oxidative chemistry of the lower atmosphere, species composition and the recycling of carbon and nutrients, etc. Plant uptake of nitrogen dioxide (NO(2)) is concentration-dependent an...
journal_title：Plant cell reports
pub_type： 杂志文章,评审
doi：10.1007/s00299-010-0898-5
更新日期：2010-10-01 00:00:00
abstract：:Independent carrot (Daucus carota) hairy root lines were established by inoculation of discs taken from the same carrot with Agrobacterium rhizogenes 8196 and A. tumefaciens C58C1(pRi8196) carrying pRi8196. Several lines were compared with respect to T-DNA length. One of them was found to have integrated sequences cov...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00270112
更新日期：1988-03-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF01088288
更新日期：1997-02-01 00:00:00
abstract：: Synthesis of five different Sudan-β-D-glucuronides (I, II, III, IV, and RedB) was performed by condensation of a set of red Sudan diazo dyes with methyl (1-deoxy-2,3,4-tri-O-acetyl-1-trichloroacetimidoyl-α-D-glucopyran)uronate. After the acid and alcohol groups had been deprotected, the resulting compounds were used ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990000219
更新日期：2000-10-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232042
更新日期：1991-06-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00272972
更新日期：1988-01-01 00:00:00
abstract：KEY MESSAGE:The zeaxanthin epoxidase gene ( MsZEP ) was cloned and characterized from alfalfa and validated for its function of tolerance toward drought and salt stresses by heterologous expression in Nicotiana tabacum. Zeaxanthin epoxidase (ZEP) plays important roles in plant response to various environment stresses d...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-015-1895-5
更新日期：2016-02-01 00:00:00
abstract：: Embryogenic nucellar callus cultures of different Citrus species and cultivars growing in hormone-free medium were transferred to medium containing either sucrose or glycerol as the only carbohydrate source. Glycerol has been reported to induce further development of Citrus somatic embryos, while in the presence of s...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990000280
更新日期：2001-01-01 00:00:00
abstract：KEY MESSAGE:We demonstrate the role of DREB1A transcription factor in better root and shoot partitioning and higher transpiration efficiency in transgenic chickpea under drought stress Chickpea (Cicer arietinum L.) is mostly exposed to terminal drought stress which adversely influences its yield. Development of cultiva...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-014-1699-z
更新日期：2015-02-01 00:00:00
abstract：:The capability of Phalaenopsis to acclimate its photosynthetic capacity and metabolic activity to cool night temperature conditions is crucial for improving orchid production in terms of efficient greenhouse heating. The extent to which Phalaenopsis possesses acclimation potential and the mechanistic background of the...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-011-1021-2
更新日期：2011-06-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00233786
更新日期：1994-12-01 00:00:00
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journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-020-02638-5
更新日期：2021-02-01 00:00:00
abstract：KEY MESSAGE:Sensitivity to Erysiphe in Noccaea praecox with low metal supply is related to the failure in enhancing SA. Cadmium protects against fungal-infection by direct toxicity and/or enhanced fungal-induced JA signaling. Metal-based defense against biotic stress is an attractive hypothesis on evolutionary advantag...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-013-1427-0
更新日期：2013-08-01 00:00:00
abstract：KEY MESSAGE:Overexpressing TaUb2 promoted stem growth and resulted in early flowering in transgenic tobacco plants. Ubiquitin are involved in the production, metabolism and proper function of gibberellin. The ubiquitin-26S proteasome system (UPS), in which ubiquitin (Ub) functions as a marker, is a post-translational r...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-012-1331-z
更新日期：2012-12-01 00:00:00
