Regulation of DNA synthesis and cell division by polyamines in Catharanthus roseus suspension cultures.

Abstract:

:Various inhibitors of polyamine biosynthesis were used to study the role of polyamines in DNA synthesis and cell division in suspension cultures of Catharanthus roseus (L.) G. Don. Arginine decarboxylase (ADC; EC 4.1.1.19) was the major enzyme responsible for putrescine production. DL α-difluoromethylarginine inhibited ADC activity, cellular putrescine content, DNA synthesis, and cell division. The effect was reversible by exogenous putrescine. Ornithine decarboxylase (ODC; EC 4.1.1.17) activity was always less than 10% of the ADC activity. Addition of DL α-difluoromethylornithine had no effect on ODC activity, cellular polyamine levels, DNA synthesis, and cell division within the first 24 h but by 48 to 72 h it did inhibit these activities. Methylglyoxal bis(guanyl-hydrazone) inhibited S-adenosylmethionine decarboxylase (EC 4.1.1.50) activity without affecting DNA synthesis and cell division.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Minocha R,Minocha SC,Komamine A,Shortle WC

doi

10.1007/BF00232042

subject

Has Abstract

pub_date

1991-06-01 00:00:00

pages

126-30

issue

3

eissn

0721-7714

issn

1432-203X

journal_volume

10

pub_type

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