Agrobacterium rhizogenes-mediated transformation of Echinacea purpurea.

Abstract:

:Echinacea purpurea seedlings were inoculated with several Agrobacterium rhizogenes strains in order to obtain hairy roots. Infection with A. rhizogenes strains LMG63 and LMG150 resulted in callus formation. Upon infection with strains ATCC 15834 and R1601 hairy roots were obtained. Opine detection confirmed transformation of E. purpurea. Comparative HPLC fingerprint analysis of the alkamides from natural plant source, control tissues, and transformed callus and roots indicated that transformed callus and hairy roots might be a promising source for continuous and standardized production of the dodeca-2E,4E,8Z,10E/Z-tetraenoic acid isobutylamide and related amides.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Trypsteen M,Van Lijsebettens M,Van Severen R,Van Montagu M

doi

10.1007/BF00236463

subject

Has Abstract

pub_date

1991-06-01 00:00:00

pages

85-9

issue

2

eissn

0721-7714

issn

1432-203X

journal_volume

10

pub_type

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