Evolution of endogenous hormone concentration in embryogenic cultures of carrot during early expression of somatic embryogenesis.

abstract：:A protocol for Agrobacterium-mediated transformation with mannose selection was developed for cotyledon petiole, hypocotyl and leaf explants of tomato (Lycopersicon esculentum L. Mill). More than 400 transgenic plants from three tomato varieties were selected with 1% mannose in combination with 0.1-0.5% glucose. Avera...

journal_title：Plant cell reports

authors： Sigareva M,Spivey R,Willits MG,Kramer CM,Chang YF

更新日期：2004-10-01 00:00:00

abstract：KEY MESSAGE:An ERF gene, LchERF , was cloned from L. chinense for the first time. Overexpression of LchERF conferred salt stress tolerance in transgenic tobacco lines during seed germination and vegetative growth. Ethylene-responsive transcription factors (ERFs) play important roles in tolerance to biotic and abiotic s...

journal_title：Plant cell reports

authors： Wu D,Ji J,Wang G,Guan C,Jin C

更新日期：2014-12-01 00:00:00

abstract：:Kanamycin-resistant plants of belladonna (Atropa belladonna) were obtained after Agrobacterium mediated transformation. When a rolC gene, which is one of the loci located on Ri plasmid of Agrobacterium rhizogenes, was co-introduced with a kanamycin resistant (NPT II) gene under control of a cauliflower mosaic virus 35...

journal_title：Plant cell reports

authors： Kurioka Y,Suzuki Y,Kamada H,Harada H

更新日期：1992-12-01 00:00:00

abstract：:Six sugarbeet (Beta vulgaris L.) lines (GWI-248, SPB-11, MonoHy 55, SMS-1, EL45 and FC607) were tested for regeneration. Shoot cultures were initiated in vitro from naked, sterilized embryos obtained from mature seed. Excised petioles from cultured shoots were plated on Gamborg's B5 medium and four modified Murashige ...

journal_title：Plant cell reports

authors： Freytag AH,Anand SC,Rao-Arelli AP,Owens LD

更新日期：1988-01-01 00:00:00

abstract：KEY MESSAGE:The Arabidopsis AP2/ERF family transcription factor AtERF15 is nuclear-localized and positively regulates ABA and stress responses. Abscisic acid (ABA) is a major plant hormone that controls the expression of hundreds genes involved in various aspects of plant growth and development, such as seed developmen...

journal_title：Plant cell reports

authors： Lee SB,Lee SJ,Kim SY

更新日期：2015-01-01 00:00:00

abstract：:Optimal protoplast yields from cotyledons (2.0×10(6) protoplasts/ 0.5 g tissue) and from true leaves (5.0×10(6) protoplasts/g tissue) of two Cucumis sativus genotypes were obtained following a 16 h digestion with, respectively, 1.25% pectinase+0.5% Cellulysin and 0.5 % pectinase+ 1.0% Cellulysin. Enzyme solutions were...

journal_title：Plant cell reports

authors： Punja ZK,Tang FA,Sarmento GG

更新日期：1990-07-01 00:00:00

abstract：:Scanning electron microscopy has been used to investigate the morphological changes occurring during the development of alfalfa somatic embryos. Embryos were initiated from callus, transferred to suspension culture and matured on solid agar medium. This developmental pattern was compared to that of zygotic embryos dev...

journal_title：Plant cell reports

authors： Xu N,Bewley JD

更新日期：1992-06-01 00:00:00

abstract：KEY MESSAGE:Transcriptome analysis of barley embryogenic callus from isolated microspore culture under salt stress uncovered a role of translation inhibition and selective activation of stress-specific proteins in cellular defense. Soil salinity is one of the major abiotic stresses which constrains the plant growth and...

journal_title：Plant cell reports

authors： Liu CH,Lu RJ,Guo GM,He T,Li YB,Xu HW,Gao RH,Chen ZW,Huang JH

更新日期：2016-08-01 00:00:00

abstract：:Albinism remains a major problem in cereal improvement programs that rely on doubled haploid (DH) technology, and the factors controlling the phenomenon are not well understood. Here we report on the positive influence of copper on the production of DH plants obtained through microspore embryogenesis (ME) in recalcitr...

journal_title：Plant cell reports

authors： Jacquard C,Nolin F,Hécart C,Grauda D,Rashal I,Dhondt-Cordelier S,Sangwan RS,Devaux P,Mazeyrat-Gourbeyre F,Clément C

更新日期：2009-09-01 00:00:00

abstract：: Identifying transformed plant lines carrying the antibiotic resistance marker gene, neomycin phosphotransferase II, requires a more definitive test than the ability of the plant to grow on kanamycin. Although a number of alternative assays have been described, most are cumbersome, time consuming and/or require the us...

journal_title：Plant cell reports

authors： McKenzie MJ,Mett V,Jameson PE

更新日期：2000-01-01 00:00:00

abstract：:Recently, five novel fluorescent proteins have been isolated from non-bioluminescent species of reef-coral organisms and have been made available through ClonTech. They are AmCyan, AsRed, DsRed, ZsGreen and ZsYellow. These proteins are valuable as reporters for transformation because they do not require a substrate or...

journal_title：Plant cell reports

authors： Wenck A,Pugieux C,Turner M,Dunn M,Stacy C,Tiozzo A,Dunder E,van Grinsven E,Khan R,Sigareva M,Wang WC,Reed J,Drayton P,Oliver D,Trafford H,Legris G,Rushton H,Tayab S,Launis K,Chang YF,Chen DF,Melchers L

更新日期：2003-11-01 00:00:00

abstract：KEY MESSAGE:Overexpression of SoSnRK2.1 improved drought tolerance and growth of tobacco plants. Sucrose non-fermenting1-related protein kinase 2 (SnRK2) is a key enzyme in regulating ABA signal transduction in plants, and it plays a significant role in response to multiple abiotic stresses. In this research, SoSnRK2.1...

journal_title：Plant cell reports

authors： Phan TT,Sun B,Niu JQ,Tan QL,Li J,Yang LT,Li YR

更新日期：2016-09-01 00:00:00

abstract：:A reliable protocol for the transformation of cultivated sunflower (Helianthus annuus L.) has been established, based on microprojectile bombardment of half shoot apices in combination with Agrobacterium tumefaciens coculture. Transgenic shoots have been obtained from 5 inbred lines, although transformation efficienci...

journal_title：Plant cell reports

authors： Knittel N,Gruber V,Hahne G,Lénée P

更新日期：1994-12-01 00:00:00

abstract：:Callus derived from stem segments of loblolly pine (Pinus taeda L.) was subcultured to media containing 0.5% of various mono-, di-, tri- or polysaccharides. None of the carbohydrate sources tested were superior to sucrose. Growth on twelve of the 20 carbohydrates tested was more than 75% of that obtained for sucrose; ...

journal_title：Plant cell reports

authors： Vuke TM,Mott RL

更新日期：1987-04-01 00:00:00

abstract：:Somatic hybrids were obtained by the symmetric fusion of embryogenic callus cells from tetraploid 'Mame' kumquat [Fortunella hindsii (Champ.) Swing.] and mesophyll cells from diploid trifoliate orange [Poncirus trifoliata (L.) Raf.]. Southern blot analysis of three regenerants revealed that they carried specific rDNA ...

journal_title：Plant cell reports

authors： Miranda M,Motomura T,Ikeda F,Ohgawara T,Saito W,Endo T,Omura M,Moriguchi T

更新日期：1997-03-01 00:00:00

abstract：:The gene coding for the green fluorescent protein (GFP) from jellyfish was introduced into conifer tissues by microprojectile bombardment and its transient expression was detected. Two versions of the GFP gene, wild-type GFP and modified GFP with a cryptic intron removed, were directly compared for their expression in...

journal_title：Plant cell reports

authors： Tian L,Séguin A,Charest PJ

更新日期：1997-02-01 00:00:00

abstract：KEY MESSAGE:Effective microspore embryogenesis in triticale is determined by a specific hormonal homeostasis: low value of IAA/cytokinins, IAA/ABA and cytokinins/ABA ratios as well as proper endogenous/exogenous auxin balance, which favours androgenic structure formation and green plant regeneration ability. The concen...

journal_title：Plant cell reports

authors： Żur I,Dubas E,Krzewska M,Waligórski P,Dziurka M,Janowiak F

更新日期：2015-01-01 00:00:00

abstract：:Leaf mesophyll protoplasts isolated from pea (Pisum sativum L.) genotypes Century and PI244253 showed transient expression of β-glucuronidase (GUS) when electroporated with plasmid DNA containing various promoter-leader sequence constructs driving the GUS gene. The optimum conditions for transient expression were: usi...

journal_title：Plant cell reports

authors： Hobbs SL,Jackson JA,Baliski DS,Delong CM,Mahon JD

更新日期：1990-06-01 00:00:00

abstract：:We developed procedures for slow-growth storage of Cedrus atlantica and Cedrus libani microcuttings of juvenile and adult origin, noting factors favouring the extension of subculture intervals. Microcuttings could be stored effectively up to 6 months at 4 degrees C and reduced light intensity, provided that they were ...

journal_title：Plant cell reports

authors： Renau-Morata B,Arrillaga I,Segura J

更新日期：2006-07-01 00:00:00

abstract：:To develop an alternative genetic transformation system that is not dependent on an antibiotic selection strategy, the phosphomannose isomerase gene (pmi) system was evaluated for producing transgenic plants of chickpea (Cicer arietinum L.). A shoot morphogenesis protocol based on the thidiazuron (TDZ)-induced shoot m...

journal_title：Plant cell reports

authors： Patil G,Deokar A,Jain PK,Thengane RJ,Srinivasan R

更新日期：2009-11-01 00:00:00

abstract：:Head-space volatiles above embryogenicPicea sitchensis (Bong.) Carr. (Sitka spruce) tissues cultured in glass Petri dishes sealed with Parafilm M or cling-film, were captured on Tenax adsorption traps and analysed by gas chromatography / mass spectrometry. Each sealing system released a single major compound into the ...

journal_title：Plant cell reports

authors： Selby C,McRoberts WC,Hamilton JT,Harvey BM

更新日期：1996-12-01 00:00:00

abstract：:The introgression of new desirable characters is very necessary in common bean (Phaseolus vulgaris L.)Interspecific hybridization of P. vulgaris L. with P. angustissimus A. Gray, a wild species with narrow leaves, could be successfully achieved for the first time using embryo culture. Sixteen to twenty-three-day old e...

journal_title：Plant cell reports

authors： Belivanis T,Doré C

更新日期：1986-10-01 00:00:00

abstract：:The aim of this study was the improvement of embryo production in wheat anther culture. Three butanol alcohols, n-butanol, sec-butanol and tert-butanol, were evaluated for their effect on microspore embryogenesis in two spring cultivars of wheat, Pavon and Caramba. Application of n-butanol, at 0.1 and 0.2% (v/v) in th...

journal_title：Plant cell reports

authors： Soriano M,Cistué L,Castillo AM

更新日期：2008-05-01 00:00:00

abstract：:We have established a high-frequency plant regeneration system via somatic embryogenesis from mature seeds of creeping bentgrass (Agrostis palustris Huds). The effects of 2,4-dichlorophenoxyacetic acid (2,4-D), 3.6-dichloroo-anisic acid (dicamba) and 6-benzyladenine (BA) on callus formation and embryogenesis were eval...

journal_title：Plant cell reports

authors： Zhong H,Srinivasan C,Sticklen MB

更新日期：1991-11-01 00:00:00

abstract：:Donor plants of Hordeum vulgare L. cv. Igri were grown in a conditioned environment to minimise fluctuations in the composition of the microspore population. After isolation different types of microspores were identified within each population, amongst others an embryogenic subpopulation. It was shown that the optimum...

journal_title：Plant cell reports

authors： Hoekstra S,van Zijderveld MH,Heidekamp F,van der Mark F

更新日期：1993-10-01 00:00:00

abstract：:The objective of this study was to correlate the time that DNA synthesis first occurs in haploid microspores of barley with cell cycle and plant morphological stages and to subsequently assess the influence of pretreatments on DNA synthesis at different stages of microspore development. Spikes with microspores in earl...

journal_title：Plant cell reports

authors： Shim YS,Kasha KJ

更新日期：2003-07-01 00:00:00

abstract：:Cell-wall invertase (CIN) catalyzes the hydrolysis of sucrose into glucose and fructose for the supply of carbohydrates to sink organs via an apoplastic pathway. To study the CIN genes in rice (Oryza sativa L.), we isolated cDNA clones showing amino acid similarity to the plant cell wall invertase proteins from a sear...

journal_title：Plant cell reports

authors： Cho JI,Lee SK,Ko S,Kim HK,Jun SH,Lee YH,Bhoo SH,Lee KW,An G,Hahn TR,Jeon JS

更新日期：2005-06-01 00:00:00

abstract：:Plants have developed diverse molecular mechanisms to resist viruses. RNA silencing plays a dominant role in antiviral defense. Recent studies have correlated plant antiviral silencing to epigenetic modification in genomic DNA and protein by remodeling the expression levels of coding genes. The plant host methylation ...

journal_title：Plant cell reports

authors： Wang C,Wang C,Zou J,Yang Y,Li Z,Zhu S

更新日期：2019-09-01 00:00:00

abstract：:Shoot and leaf segments of a non-regenerable Medicago sativa L. genotype were cocultivated with the "shooty" mutant of Agrobacterium tumefaciens carrying the pGV 2206 plasmid. Transformed callus lines were selected and regenerated on the hormone free B5 medium. Southern blot analysis demonstrated integration of T-DNA ...

journal_title：Plant cell reports

authors： Kuchuk N,Komarnitski I,Shakhovsky A,Gleba Y

更新日期：1990-04-01 00:00:00

abstract：:Protoplasts were isolated from embryogenic callus ofAbies alba L. which originated from immature seeds. The protoplasts were immobilized in alginate beads in order to follow the development of single protoplasts.Surrounding culture medium was modified from Kao and Michayluk (1975). After cell wall regeneration subsequ...

journal_title：Plant cell reports

authors： Lang H,Kohlenbach HW

更新日期：1989-02-01 00:00:00