Abstract:
:Rice protoplasts (Oryza sativa L. v Taipei 309) have been transformed to kanamycin resistance following uptake of pCaMVNEO induced by electroporation, PEG and PEG combined with electroporation. Protoplast-derived colonies selected on medium containing 100 μg/ml of kanamycin expressed NPTII activity, and contained DNA that hybridised to a 1.0 Kb BamHI fragment of pCaMVNEO carrying the NPTII gene. Expression of the transformation frequency in relative terms (number of kanamycin resistant colonies compared to the number of colonies on kanamycin free medium) gave frequencies of 26.1%, 8.5% and 2.9% following electroporation, PEG and PEG with electroporation respectively. In absolute terms (number of kanamycin resistant colonies compared to the number of protoplasts plated) these represent frequencies of 19.9×10(-5), 9.0×10(-5) and 2.7×10(-5) for the three procedures.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Yang H,Zhang HM,Davey MR,Mulligan BJ,Cocking ECdoi
10.1007/BF00269528subject
Has Abstractpub_date
1988-10-01 00:00:00pages
421-5issue
6eissn
0721-7714issn
1432-203Xjournal_volume
7pub_type
杂志文章abstract::An early identification of fusion products was based on the presumed vigorous growth of hybrid calluses after fusion between Solanum brevidens and S. tuberosum leaf protoplasts. The S. brevidens protoplasts were unable to form multicellular colonies under the applied culture conditions. Three size groups of calluses w...
journal_title:Plant cell reports
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journal_title:Plant cell reports
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更新日期:1993-12-01 00:00:00
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更新日期:1995-05-01 00:00:00
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更新日期:2003-03-01 00:00:00
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journal_title:Plant cell reports
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更新日期:2014-11-01 00:00:00
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journal_title:Plant cell reports
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更新日期:1987-02-01 00:00:00
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更新日期:1986-04-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
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更新日期:1988-06-01 00:00:00
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