Abstract:
:Plant regeneration via somatic embryogenesis was achieved in callus cultures derived from nodal explants of in vitro grown seedlings and excised mature zygotic embryos of three bamboo species on Murashige and Skoog's (MS) basal medium supplemented with 0.5 mg/l kinetin (Kn), 2.0 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D), 10 mg/l adenine sulphate (Ads) and 3% (w/v) sucrose incubated in the light or in the dark. Somatic embryos germinated (95-98%) into normal plants and were transferred to soil with 95% success. In vitro flowering was induced on shoots developed from nodal explants taken from somatic embryo regenerated plants of Bambusa vulgaris, Dendrocalamus giganteus and Dendrocalamus strictus on half-strength MS basal medium supplemented with 0.25 mg/l indole-3-butyric acid (IBA), 0.5 mg/l Ads, 0.5 mg/l gibberellic acid (GA3) and 3% sucrose.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Rout GR,Das Pdoi
10.1007/BF00231624subject
Has Abstractpub_date
1994-09-01 00:00:00pages
683-6issue
12eissn
0721-7714issn
1432-203Xjournal_volume
13pub_type
杂志文章abstract::Morphologically normal and fertile transgenic plants of mungbean with two transgenes, bar and alpha-amylase inhibitor, have been developed for the first time. Cotyledonary node explants were transformed by cocultivation with Agrobacterium tumefaciens strain EHA105 harboring a binary vector pKSB that carried bialaphos ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-006-0224-4
更新日期:2007-02-01 00:00:00
abstract::Cell structures were compared between alkaloid-producing and non-producing cell cultures of Coptis japonica and Thalictrum minus by electron microscopic observation. In alkaloid-producing cells of C. japonica, prior to the onset of alkaloid synthesis, the vacuoles showed a greater volume than in non-producing cells. T...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269721
更新日期:1986-02-01 00:00:00
abstract::This study aimed at establishing a protocol to increase the number of regenerated shoots and to limit the recovery of "escapes" during the regeneration of transgenic flax plants (cv Barbara). Here, we describe how light, adapted media and selection scheme could stimulate the transformation process, the organogenic pot...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-008-0638-2
更新日期:2009-03-01 00:00:00
abstract:KEY MESSAGE:Autotetraploid Arabidopsis line esd and 4COL exhibit enhanced tolerance to Cu stress by enhancing activation of antioxidative defenses, altering expression of genes related to Cu transport, chelation, and ABA-responsive. Autopolyploidy is ubiquitous among angiosperms and often results in better adaptation t...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-017-2176-2
更新日期:2017-10-01 00:00:00
abstract::This article describes conditions to optimize the yield of viable protoplasts from callus tissue of Asparagus densiflorus cv. Sprengeri and their subsequent regeneration into plantlets. Callus tissue was initiated by culturing spear sections (5-7 mm) on Murashige and Skoog (MS) medium supplemented with 0.8% (wt/vol) B...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050364
更新日期:1997-12-01 00:00:00
abstract::Somatic embryogenesis was induced in callus tissues derived from young flower buds ofPanax notoginseng via callus within 18 weeks of culture. The mature somatic embryos were germinated on half-strength Murashige and Skoog's (MS) medium supplemented with gibberellic acid A3(GA) and 6-benzyladenine (BA). The most suitab...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF01092764
更新日期:1997-04-01 00:00:00
abstract::We developed an efficient plant regeneration system from protoplasts for poplar (Populus alba L.). Protoplasts were isolated from 4-day-old suspension cultures derived from seed-induced calli with a yield of 6.96× 106 cells/g fresh weight cells and then cultured at a concentration of 2.5×105 cells/ml in NH4NO3-free Mu...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050378
更新日期:1998-01-01 00:00:00
abstract::Expression of the green fluorescent protein (gfp) gene, under regulatory control of either the constitutive 35S promoter or the developmentally-regulated lectin promoter was monitored and quantified using a newly-developed automated tracking system. The automated system consisted of a computer-controlled two-dimension...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-006-0142-5
更新日期:2006-09-01 00:00:00
abstract::In vitro adventitious shoot formation was examined in petioles and laminae ofPaulownia elongata, P. fortunei, andP. 'Henan 1'. Fully expanded, dark green, thick, older leaves were healthier and exhibited greater callus and shoot production than did young leaves. The ten clones used varied greatly in adventitious shoot...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF01088288
更新日期:1997-02-01 00:00:00
abstract::Rhodamine-6G(R6G), a lipophilic dye which degrades mammalian mitochondria, was shown to arrest the division of Nicotiana protoplasts. When albino recipient-protoplasts were treated with R6G and fused with X-irradiated (green) donor- protoplasts, only green cybrid plants were obtained. The mtDNA of the cybrids was anal...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269126
更新日期:1986-06-01 00:00:00
abstract::Callus cultures and cell suspension cultures derived from Ginkgo biloba L. leaves produced ginkgolidc B. In cell suspension cultures, the production reached a maximum by the 13th day of subculture and followed by a sharp decrease. The medium of Murashige and Skoog induced the highest ginkgolide B content in cultures w...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232783
更新日期:1995-05-01 00:00:00
abstract::Cultured Catharanthus roseus cells exhibit transmembrane ferricyanide reduction through a plasma membrane redox system which may be associated with proton translocation. Evidence shows that endogenous pyridine nucleotides serve as hydrogen donors for the reaction. The proton translocating function of the redox system ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269886
更新日期:1985-12-01 00:00:00
abstract:KEY MESSAGE:Our data present H 2 S in a new role, serving as a multi-faceted transducer to different response mechanisms during NO-induced acquisition of tolerance to flooding-induced hypoxia in maize seedling roots. Nitric oxide (NO), serving as a secondary messenger, modulates physiological processes in plants. Recen...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-016-2037-4
更新日期:2016-11-01 00:00:00
abstract::Leaf mesophyll protoplasts isolated from pea (Pisum sativum L.) genotypes Century and PI244253 showed transient expression of β-glucuronidase (GUS) when electroporated with plasmid DNA containing various promoter-leader sequence constructs driving the GUS gene. The optimum conditions for transient expression were: usi...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232127
更新日期:1990-06-01 00:00:00
abstract::An efficient plant regeneration system from leaf-derived callus in 6 genotypes of Avena sativa L. has been established. Regenerable callus was induced in the basal 1-2 mm region of 2 to 5 day old seedlings. Plants were regenerated from the regenerable callus and grown to maturity. The frequency of regenerable callus f...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00238604
更新日期:1995-03-01 00:00:00
abstract:KEY MESSAGE:Overexpression of SoSnRK2.1 improved drought tolerance and growth of tobacco plants. Sucrose non-fermenting1-related protein kinase 2 (SnRK2) is a key enzyme in regulating ABA signal transduction in plants, and it plays a significant role in response to multiple abiotic stresses. In this research, SoSnRK2.1...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-016-2004-0
更新日期:2016-09-01 00:00:00
abstract:KEY MESSAGE:SbNAC genes (131) encoding 183 proteins were identified from the sorghum genome and characterized. The expression patterns of SbSNACs were evaluated at three sampling time points under post-flowering drought stress. NAC proteins are specific transcription factors in plants, playing vital roles in developmen...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-019-02371-8
更新日期:2019-03-01 00:00:00
abstract::An improved isolated microspore culture protocol alleviating the recalcitrance typically observed in six-row spring barley was developed by optimizing four key physical factors to increase embryogenesis and reduce albinism. Doubled haploid (DH) plants are completely homozygous individuals that can be generated in just...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-014-1583-x
更新日期:2014-06-01 00:00:00
abstract::Using a conventional sexual crossing technique, Nicotiana tabacum x N. acuminata was not produced. After the fertilized ovules were cultured for 20 days in a liquid Nitsch H medium, germination was observed. The roots grew rapidly but leaves did not. However, plantlets were produced in an H medium containing Benzylade...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00268613
更新日期:1986-10-01 00:00:00
abstract::Plants were regenerated from leafy spurge (Euphorbia esula L.) cell suspensions obtained from stem callus. A North Dakota accession was highly regenerable, but two accessions from Oregon and Austria formed only a few plantlets. Organogenesis occurred in media without growth regulators, under fluorescent lights (30 to ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00272536
更新日期:1988-06-01 00:00:00
abstract:KEY MESSAGE:The recovery of non-functional-enhanced green fluorescence protein can be used as indicator to facilitate the identification of mutants generated by CRISPR/Cas9. The CRISPR/Cas9 system is a powerful tool for genome editing and it has been employed to knock out genes of interest in multiple plant species. Id...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-019-02465-3
更新日期:2019-12-01 00:00:00
abstract:KEY MESSAGE:We report global gene expression patterns of poplar in response to chilling stress. A total of 1,085 significantly differentially expressed genes, involved in photosynthesis, signal transduction, and regulation of transcription, were identified. To understand the gene network underlying the response to chil...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-013-1454-x
更新日期:2013-09-01 00:00:00
abstract::A biolistic particle delivery system was used to genetically transform embryogenic tissue of Pinus radiata. The introduced DNA contained a uidA reporter gene under the control of either the tandem CaMV 35S or the artificial Emu promoter, and the npt II selectable marker controlled by the CaMV 35S promoter. The average...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050426
更新日期:1998-04-01 00:00:00
abstract::Plant regeneration in Arachis pintoi was obtained via two developmental pathways: organogenesis and somatic embryogenesis. Organogenic callus cultures were initiated from pieces of leaf on MS medium supplemented with NAA or 2,4-D in combination with BA, KIN or 2iP. The most suitable combination for plant regeneration ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990000198
更新日期:2000-09-01 00:00:00
abstract::Companion cells are metabolically active and functionally specialized cells that behave as terminals for the transport of materials between phloem and the surrounding tissue. Although previous research has clarified the distinct function of companion cells, it is still largely unknown how plants establish and maintain...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-011-1080-4
更新日期:2011-09-01 00:00:00
abstract::Polyclonal antibodies against a part of pea (Pisum sativum L.) LOXG protein have been raised to study the pattern of distribution of related lipoxygenases in pea carpels. The antiserum recognized three lipoxygenase polypeptides in carpels. One of them became undetectable 24 hours after fruit development induction, sug...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232465
更新日期:1996-04-01 00:00:00
abstract::A protocol is described for rapid and large-scale propagation of the woody aromatic and medicinal shrub Vitex negundo by in vitro culture of nodal segments from mature plants. Of the three different cytokinins - N6-benzyladenine (BA), kinetin, and thidiazuron - evaluated as supplements to Murashige and Skoog (MS) medi...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050576
更新日期:1998-12-01 00:00:00
abstract::Efficient protoplast culture and plant regeneration of five U.S. rice cultivars (Oryza sativa L.) - Mercury, Lacassine, Maybelle, Cypress, and Lemont - were obtained from suspension cells maintained in modified General Medium. Embryogenic suspension cells were developed from calli grown on the original callus inductio...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF01690249
更新日期:1995-01-01 00:00:00
abstract::To study the developmental transition of chloroplasts from C(3) to C(4) photosynthesis in the terrestrial single-cell C(4) species Bienertia sinuspersici, a regeneration protocol was developed. Stem explant material developed callus either with or without red nodular structures (RNS) when cultured on Murashige-Skoog (...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-011-1067-1
更新日期:2011-08-01 00:00:00
abstract::The activity of a predicted promoter, PMC8, from Milk vetch dwarf virus was evaluated by comparing it with the cauliflower mosaic virus 35S RNA promoter (P35S) and PNCR, a promoter from Soybean chlorotic mottle virus. When the GUS fusion gene was introduced into tobacco, PMC8 showed a similar expression profile to P35...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-005-0917-0
更新日期:2005-06-01 00:00:00