Somatic embryogenesis and in vitro flowering of 3 species of bamboo.

Abstract:

:Plant regeneration via somatic embryogenesis was achieved in callus cultures derived from nodal explants of in vitro grown seedlings and excised mature zygotic embryos of three bamboo species on Murashige and Skoog's (MS) basal medium supplemented with 0.5 mg/l kinetin (Kn), 2.0 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D), 10 mg/l adenine sulphate (Ads) and 3% (w/v) sucrose incubated in the light or in the dark. Somatic embryos germinated (95-98%) into normal plants and were transferred to soil with 95% success. In vitro flowering was induced on shoots developed from nodal explants taken from somatic embryo regenerated plants of Bambusa vulgaris, Dendrocalamus giganteus and Dendrocalamus strictus on half-strength MS basal medium supplemented with 0.25 mg/l indole-3-butyric acid (IBA), 0.5 mg/l Ads, 0.5 mg/l gibberellic acid (GA3) and 3% sucrose.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Rout GR,Das P

doi

10.1007/BF00231624

subject

Has Abstract

pub_date

1994-09-01 00:00:00

pages

683-6

issue

12

eissn

0721-7714

issn

1432-203X

journal_volume

13

pub_type

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