The promoter of Milk vetch dwarf virus component 8 confers effective gene expression in both dicot and monocot plants.

Abstract:

:The activity of a predicted promoter, PMC8, from Milk vetch dwarf virus was evaluated by comparing it with the cauliflower mosaic virus 35S RNA promoter (P35S) and PNCR, a promoter from Soybean chlorotic mottle virus. When the GUS fusion gene was introduced into tobacco, PMC8 showed a similar expression profile to P35S but with a more intense expression in proliferating tissues. The usefulness of PMC8 was confirmed by driving NPTII for selection of kanamycin-resistant tobacco plants with improved transformation efficiency. PMC8 was also effective in transgenic rice plants. Thus, PMC8 is useful as an alternative to P35S in both dicotyledonous and monocotyledonous plants, especially for gene expression in proliferating tissues.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Shirasawa-Seo N,Sano Y,Nakamura S,Murakami T,Gotoh Y,Naito Y,Hsia CN,Seo S,Mitsuhara I,Kosugi S,Ohashi Y

doi

10.1007/s00299-005-0917-0

keywords:

subject

Has Abstract

pub_date

2005-06-01 00:00:00

pages

155-63

issue

3

eissn

0721-7714

issn

1432-203X

journal_volume

24

pub_type

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