Selection for NaCl tolerance in cell cultures of Medicago sativa and recovery of plants from a NaCl-tolerant cell line.

abstract：:Autoclaving is a standard procedure for sterilizing nutrient media for plant tissue cultures. Most tissue cultures are grown at pH 5.2 to 5.8 with pH adjustments being made prior to autoclaving. This paper reports that there are significant differences between initial pH levels and pH levels following autoclaving, par...

journal_title：Plant cell reports

authors： Skirvin RM,Chu MC,Mann ML,Young H,Sullivan J,Fermanian T

更新日期：1986-08-01 00:00:00

abstract：:Additional virulence (vir) genes in Agrobacterium tumefaciens and sonication were investigated for their impact on transformation efficiency in loblolly pine (Pinus taeda L.). Mature zygotic embryos of loblolly pine were co-cultivated with disarmed A. tumefaciens strain EHA105 containing either plasmid vector pCAMBIA1...

journal_title：Plant cell reports

authors： Tang W

更新日期：2003-02-01 00:00:00

abstract：KEY MESSAGE:A R2R3-MYB transcription factor EsAN2 was isolated from Epimedium sagittatum and functionally characterized to regulate the anthocyanin biosynthetic pathway. Epimedium plants are used widely both as traditional Chinese medicinal herbs and ornamental perennials. Anthocyanins, acting as major contributors to ...

journal_title：Plant cell reports

authors： Huang W,Khaldun AB,Lv H,Du L,Zhang C,Wang Y

更新日期：2016-04-01 00:00:00

abstract：UNLABELLED:Calmodulin-binding proteins (CBPs) have been known to be involved in both biotic and abiotic stress responses. Recently, two closely related CBPs, Arabidopsis SAR Deficient 1 and CBP60g, were found to belong to a new family of transcription factors that regulate salicylic acid (SA) biosynthesis triggered by ...

journal_title：Plant cell reports

authors： Wan D,Li R,Zou B,Zhang X,Cong J,Wang R,Xia Y,Li G

更新日期：2012-07-01 00:00:00

abstract：:Factors involved in promoting caulogenesis from hypocotyl explants of Phyllanthus fraternus were studied. Hypocotyl explants were cultured on B5 medium supplemented with 2,4-D or NAA in the presence and absence of BAP (at concentrations 0, 10(-7), 10(-6) and 10(-5)M). Adventitious shoots differentiated from callus dev...

journal_title：Plant cell reports

authors： Rajasubramaniam S,Pardha Saradhi P

更新日期：1994-08-01 00:00:00

abstract：: Cells of a suspension culture of Doritaenopsis cv. New Toyohashi were placed in a mixture of 2 M glycerol and 0.4 M sucrose for 15 min at room temperature and then dehydrated with a vitrification solution (PVS2) for 1-3 h on ice and plunged into liquid nitrogen. The highest viability (64% by 2,3,5-triphenyltetrazoliu...

journal_title：Plant cell reports

authors： Tsukazaki H,Mii M,Tokuhara K,Ishikawa K

更新日期：2000-12-01 00:00:00

abstract：:The effects of NaCl, feeder cells and the embedding of protoplasts in calcium alginate have been investigated in an attempt to improve culture conditions of recalcitrant sugar beet (Beta vulgaris L.) mesophyll protoplasts. While the use of NaCl in all instances proved detrimental to protoplast development, the other t...

journal_title：Plant cell reports

authors： Hall RD,Pedersen C,Krens FA

更新日期：1993-04-01 00:00:00

abstract：KEY MESSAGE:SbNAC genes (131) encoding 183 proteins were identified from the sorghum genome and characterized. The expression patterns of SbSNACs were evaluated at three sampling time points under post-flowering drought stress. NAC proteins are specific transcription factors in plants, playing vital roles in developmen...

journal_title：Plant cell reports

authors： Sanjari S,Shirzadian-Khorramabad R,Shobbar ZS,Shahbazi M

更新日期：2019-03-01 00:00:00

abstract：:Various inhibitors of polyamine biosynthesis were used to study the role of polyamines in DNA synthesis and cell division in suspension cultures of Catharanthus roseus (L.) G. Don. Arginine decarboxylase (ADC; EC 4.1.1.19) was the major enzyme responsible for putrescine production. DL α-difluoromethylarginine inhibite...

journal_title：Plant cell reports

authors： Minocha R,Minocha SC,Komamine A,Shortle WC

更新日期：1991-06-01 00:00:00

abstract：:The influence of light on the expression of NADPH: protochlorophyllide oxidoreductase has been studied in different plant species. The presumptive precursors to this enzyme have been characterized by in vitro translation of poly (A) RNA and immunoprecipitation. Two bands of apparent molecular weights of about 42 000 a...

journal_title：Plant cell reports

authors： Meyer G,Bliedung H,Kloppstech K

更新日期：1983-02-01 00:00:00

abstract：:A procedure has been developed for the clonal propagation of Picrorhiza kurroa Royle ex Benth. through shoot tip culture. Murashige and Skoog's medium (1962) supplemented with kinetin (3.0 to 5.0 mg/l) supported rapid proliferation of multiple shoots from the explants. Addition of indole-3-acetic acid (1.0 mg/l) to th...

journal_title：Plant cell reports

authors： Lal N,Ahuja PS,Kukreja AK,Pandey B

更新日期：1988-05-01 00:00:00

abstract：:The tobacco cell line TX1 (Nicotiana tabacum) accumulates up to 10 mg cinnamoyl putrescines/g dry weight. This level was further increased under various growth limiting conditions. The negative effect of accumulated phosphate on the optimal expression of cinnamoyl putrescine biosynthesis was prevented by the fedbatch ...

journal_title：Plant cell reports

authors： Schiel O,Jarchow-Redecker K,Piehl GW,Lehmann J,Berlin J

更新日期：1984-02-01 00:00:00

abstract：:MicroRNAs (miRNAs) are small non-coding RNAs that play vital roles in plant abiotic stress responses via cleavage or translational inhibition of their target mRNAs. Populus euphratica is a typical stress-resistant sessile organism that grows in desert areas. Here, we identified sequences of 12 miRNA precursors from 11...

journal_title：Plant cell reports

authors： Qin Y,Duan Z,Xia X,Yin W

更新日期：2011-10-01 00:00:00

abstract：:Pyrolysis mass spectrometry (PyMS) is a rapid, simple, high-resolution analytical method based on thermal degradation of complex material in a vacuum and has been widely applied to the discrimination of closely related microbial strains. Leaf samples of six species and one variety of higher plants (Rosa multiflora, R....

journal_title：Plant cell reports

authors： Kim SW,Ban SH,Chung HJ,Choi DW,Choi PS,Yoo OJ,Liu JR

更新日期：2004-02-01 00:00:00

abstract：KEY MESSAGE:Transcriptomes of two switchgrass genotypes representing the upland and lowland ecotypes will be key tools in switchgrass genome annotation and biotic and abiotic stress functional genomics. Switchgrass (Panicum virgatum L.) is an important bioenergy feedstock for cellulosic ethanol production. We report ge...

journal_title：Plant cell reports

authors： Ayyappan V,Saha MC,Thimmapuram J,Sripathi VR,Bhide KP,Fiedler E,Hayford RK,Kalavacharla VK

更新日期：2017-01-01 00:00:00

abstract：:The expression and functionality of a resveratrol synthase (RS) gene from peanut (Arachis hypogaea) was studied using an Agrobacterium tumefaciens-mediated transient expression system in Nicotiana benthamiana leaves. Functional analysis of RS was demonstrated by tracking its expression during 96 h. To measure the tran...

journal_title：Plant cell reports

authors： Condori J,Medrano G,Sivakumar G,Nair V,Cramer C,Medina-Bolivar F

更新日期：2009-04-01 00:00:00

abstract：: An Agrobacterium tumefaciens-mediated transformation method has been developed for onions (Allium cepa L.) using immature embryos as the explant source. Transgenic plants were recovered from the open-pollinated onion cultivar Canterbury Longkeeper at a maximum transformation frequency from immature embryos of 2.7%. T...

journal_title：Plant cell reports

authors： Eady CC,Weld RJ,Lister CE

更新日期：2000-03-01 00:00:00

abstract：:Hypocotyl explants of three leguminous forest tree species, Albizia amara, A. lucida and A. richardiana, have differentiated shoot buds on B5 basal medium. Maximum number of shoots per explant developed on basal medium augmented with 2,4-D (0.1 μM) in A. amara (2) and BA (10 μM) for both A. lucida (2) and A. richardia...

journal_title：Plant cell reports

authors： Tomar UK,Gupta SC

更新日期：1988-10-01 00:00:00

abstract：KEY MESSAGE:Transcript profiling during the early induction phase of oil palm tissue culture and RNAi studies in a model somatic embryogenesis system showed that EgENOD93 expression is essential for somatic embryogenesis. Micropropagation of oil palm through tissue culture is vital for the generation of superior and un...

journal_title：Plant cell reports

authors： Chan PL,Rose RJ,Abdul Murad AM,Zainal Z,Ong PW,Ooi LC,Low EL,Ishak Z,Yahya S,Song Y,Singh R

更新日期：2020-11-01 00:00:00

abstract：:Quantitative trait loci (QTLs) associated with androgenic responsiveness in triticale were analyzed using a population of 90 DH lines derived from the F1 cross between inbred line 'Saka 3006' and cv. 'Modus', which was used in a number of earlier studies on molecular mapping in this crop. Using Windows QTL Cartographe...

journal_title：Plant cell reports

authors： Krzewska M,Czyczyło-Mysza I,Dubas E,Gołębiowska-Pikania G,Golemiec E,Stojałowski S,Chrupek M,Zur I

更新日期：2012-11-01 00:00:00

abstract：:Green fluorescent protein (GFP) has been used widely as a powerful bioluminescent reporter, but its visualization by existing methods in tissues or whole plants and its utilization for high-throughput screening remains challenging in many species. Here, we report a fluorescence image analyzer-based method for GFP dete...

journal_title：Plant cell reports

authors： Hily JM,Liu Z

更新日期：2009-03-01 00:00:00

abstract：:Shoot cultures ofAtractylodes lancea DC. (Compositae) have been established by inoculating the flower bud on Linsmaier-Skoog's medium supplemented with 10(-5) M naphthaleneacetic acid and 10(-5) M 6-benzyl-aminopurine. Shoots were multiplied on a medium containing 10(-6) M 6-benzylaminopurine. Propagated shoots rooted...

journal_title：Plant cell reports

authors： Hiraoka N,Yamada N,Kodama T,Tomita Y

更新日期：1984-06-01 00:00:00

abstract：KEY MESSAGE:A novel Zoysia japonica salt-induced glycine-rich RNA-binding protein gene was cloned in this study and its overexpression caused salt sensitivity in transgenic Arabidopsis. Glycine-rich RNA-binding proteins (GRPs) play crucial roles in diverse plant developmental processes. However, the mechanisms and func...

journal_title：Plant cell reports

authors： Teng K,Tan P,Xiao G,Han L,Chang Z,Chao Y

更新日期：2017-01-01 00:00:00

abstract：KEY MESSAGE:SnYSL3 encodes a plasma-localized transporter delivering various metal-nicotianamine complexes. The expression of SnYSL3 is up-regulated by excess Cd, suggesting an important role for SnYSL3 in response to Cd stress. The Yellow Stripe-Like (YSL) transporters have been proposed to participate in metal uptake...

journal_title：Plant cell reports

authors： Feng S,Tan J,Zhang Y,Liang S,Xiang S,Wang H,Chai T

更新日期：2017-02-01 00:00:00

abstract：:Plant regeneration from immature embryos of peanut (Arachis hypogaea L.) can be accomplished through somatic embryogenesis. The highest frequency of somatic embryo formation occurred on B5 medium plus 0.5-1.0 mg/l picloram. Shoots and plants developed from the somatic embryos only after extended culture on basal mediu...

journal_title：Plant cell reports

authors： Ozias-Akins P

更新日期：1989-04-01 00:00:00

abstract：:Molecular analysis of gene expression differences between green and red lettuce leaves was performed using the SSH method. BlastX comparisons of subtractive expressed sequence tags (ESTs) indicated that 7.6% of clones encoded enzymes involved in secondary metabolism. Such clones had a particularly high abundance of fl...

journal_title：Plant cell reports

authors： Park JS,Choung MG,Kim JB,Hahn BS,Kim JB,Bae SC,Roh KH,Kim YH,Cheon CI,Sung MK,Cho KJ

更新日期：2007-04-01 00:00:00

abstract：KEY MESSAGE:An ERF gene, LchERF , was cloned from L. chinense for the first time. Overexpression of LchERF conferred salt stress tolerance in transgenic tobacco lines during seed germination and vegetative growth. Ethylene-responsive transcription factors (ERFs) play important roles in tolerance to biotic and abiotic s...

journal_title：Plant cell reports

authors： Wu D,Ji J,Wang G,Guan C,Jin C

更新日期：2014-12-01 00:00:00

abstract：:Novel and efficient protocols for plant regeneration and genetic transformation from longitudinally-halved cotyledons ofin vitro raised seedlings in eggplant (Solanum melongena L.) are described. After co-cultivation withAgrobacterium vectors harboring neomycin phosphotransferase (nptll) as selectable marker, transgen...

journal_title：Plant cell reports

authors： Fári M,Nagy I,Csányi M,Mitykó J,Andrásfalvy A

更新日期：1995-01-01 00:00:00

abstract：:A novel member of the AP2/ERF transcription factor family, SlERF5, was identified from a tomato mature leaf cDNA library screen. The complete DNA sequence of SlERF5 encodes a putative 244-amino acid DNA-binding protein which most likely acts as a transcriptional regulator and is a member of the ethylene responsive fac...

journal_title：Plant cell reports

authors： Pan Y,Seymour GB,Lu C,Hu Z,Chen X,Chen G

更新日期：2012-02-01 00:00:00

abstract：:A protocol previously developed for B. napus microspore culture was modified to produce embryos from several lines of Brassica campestris. Bud size, genotype, media constituents, and incubation time and temperature were examined. Donor plants were grown in a growth cabinet at a day/night temperature of 10/5°C. Microsp...

journal_title：Plant cell reports

authors： Baillie AM,Epp DJ,Hutcheson D,Keller WA

更新日期：1992-06-01 00:00:00