Abstract:
:Molecular analysis of gene expression differences between green and red lettuce leaves was performed using the SSH method. BlastX comparisons of subtractive expressed sequence tags (ESTs) indicated that 7.6% of clones encoded enzymes involved in secondary metabolism. Such clones had a particularly high abundance of flavonoid-metabolism proteins (6.5%). Following SSH, 566 clones were rescreened for differential gene expression using dot-blot hybridization. Of these, 53 were found to overexpressed during red coloration. The up-regulated expression of six genes was confirmed by Northern blot analyses. The expression of chalcone synthase (CHS), flavanone 3-hydroxylase (F3H), and dihydroflavonol 4-reductase (DFR) genes showed a positive correlation with anthocyanin accumulation in UV-B-irradiated lettuce leaves; flavonoid 3',5'-hydroxylase (F3',5'H) and anthocyanidin synthase (ANS) were expressed continuously in both samples. These results indicated that the genes CHS, F3H, and DFR coincided with increases in anthocyanin accumulation during the red coloration of lettuce leaves. This study show a relationship between red coloration and the expression of up-regulated genes in lettuce. The subtractive cDNA library and EST database described in this study represent a valuable resource for further research for secondary metabolism in the vegetable crops.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Park JS,Choung MG,Kim JB,Hahn BS,Kim JB,Bae SC,Roh KH,Kim YH,Cheon CI,Sung MK,Cho KJdoi
10.1007/s00299-006-0255-xsubject
Has Abstractpub_date
2007-04-01 00:00:00pages
507-16issue
4eissn
0721-7714issn
1432-203Xjournal_volume
26pub_type
杂志文章abstract::Conditions favourable to embryogenesis from isolated microspores of Brassica rapa L. ssp. oleifera (canola quality) were identified. A population with enhanced responsiveness for microspore embryogenesis (C200) was synthesized by crossing individual plants showing microspore embryogenic potential. For optimal microspo...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232537
更新日期:1992-05-01 00:00:00
abstract::Cell structures were compared between alkaloid-producing and non-producing cell cultures of Coptis japonica and Thalictrum minus by electron microscopic observation. In alkaloid-producing cells of C. japonica, prior to the onset of alkaloid synthesis, the vacuoles showed a greater volume than in non-producing cells. T...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269721
更新日期:1986-02-01 00:00:00
abstract::A new method of estimating the amount of berberine released from minute cell colonies of Thalictrum minus has been devised to facilitate the selection of high berberine-producing cell lines. In this system, cell aggregates obtained from a cell suspension culture are grown on small pieces of an agar culture medium and ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00268477
更新日期:1987-06-01 00:00:00
abstract::Pyrolysis mass spectrometry (PyMS) is a rapid, simple, high-resolution analytical method based on thermal degradation of complex material in a vacuum and has been widely applied to the discrimination of closely related microbial strains. Leaf samples of six species and one variety of higher plants (Rosa multiflora, R....
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-003-0714-6
更新日期:2004-02-01 00:00:00
abstract:KEY MESSAGE:The cytokinin pathway promotes the initiation of bulbil formation, and iPA may an important type of cytokinin during bulbil formation in Lilium lancifolium. Bulbils are important vegetative reproductive organs in triploid Lilium lancifolium. We previously showed that cytokinins are involved in bulbil format...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-020-02535-x
更新日期:2020-07-01 00:00:00
abstract::Head-space volatiles above embryogenicPicea sitchensis (Bong.) Carr. (Sitka spruce) tissues cultured in glass Petri dishes sealed with Parafilm M or cling-film, were captured on Tenax adsorption traps and analysed by gas chromatography / mass spectrometry. Each sealing system released a single major compound into the ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF01890865
更新日期:1996-12-01 00:00:00
abstract::The full-length cDNAs of eight S ribonucleases (S-RNases) were cloned from stylar RNA of European pear cultivars that could not be characterized by the cleaved amplified polymorphic sequences (CAPS) marker system for genotyping European pear cultivars harboring nine S alleles Sa, Sb, Sd, Se, Sh, Sk, Sl, Sq, and Sr. Co...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-006-0254-y
更新日期:2007-03-01 00:00:00
abstract::Plum hypocotyl slices were transformed with the coat protein (CP) gene of plum pox virus (PPV-CP) following cocultivation with Agrobacterium tumefaciens containing the plasmid pGA482GG/PPVCP-33. This binary vector carries the PPV-CP gene construct, as well as the chimeric neomycin phosphotransferase and β-glucuronidas...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00233291
更新日期:1994-11-01 00:00:00
abstract::Green plants were repeatedly regenerated from suspension-derived protoplasts of Kentucky blue grass (Poa pratensis L.) cv. "Geronimo". One suspension was capable of donating competent protoplasts during long-term culture i. e. 10-16 months after its establishment. The plating efficiency of the protoplasts from three d...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00233055
更新日期:1993-08-01 00:00:00
abstract::The AtGRP5 gene from Arabidopsis thaliana encodes a glycine-rich protein which has a major activity in protoderm-derived cells and is expressed in cells that undergo the first anatomical modifications leading to somatic embryo development. It has been previously demonstrated that its minimum promoter is 316 bp long in...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-010-0835-7
更新日期:2010-05-01 00:00:00
abstract::Fully fertile plants with the expected chromosome number 2n=40 were regenerated from excised leaf sections of Glycine clandestina. Shoots formed directly on the explants through organogenesis. Utilizing the same media and procedures fully fertile plants were also regenerated from cotyledon and hypocotyl tissue of the ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269116
更新日期:1986-06-01 00:00:00
abstract::Plants have their own mechanisms for overcoming various stresses. In cold regions, plants are subject to stress and must enter an inherent dormancy, through several complex mechanisms, if they are to continue to exist. In winter, regulation of tonoplast and plasma membrane aquaporin genes differed in the bud cushions ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-009-0770-7
更新日期:2009-11-01 00:00:00
abstract::Microspore-derived embryos from Brassica napus L. were dried to less than 15% moisture and stored dry for a minimum of 7 days. Successful plant regeneration was observed when embryos at the cotyledonary stage of development were treated with 50 uM ABA for 7 days prior to desiccation. Solid agar or liquid medium gave s...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00193155
更新日期:1991-09-01 00:00:00
abstract:KEY MESSAGE:The Arabidopsis AP2/ERF family transcription factor AtERF15 is nuclear-localized and positively regulates ABA and stress responses. Abscisic acid (ABA) is a major plant hormone that controls the expression of hundreds genes involved in various aspects of plant growth and development, such as seed developmen...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-014-1688-2
更新日期:2015-01-01 00:00:00
abstract::In this study, we present a method for transient expression of the type III effector AvrGf1 from Xanthomonas citri subsp. citri strain A(w) in grapefruit leaves (Citrus paradisi) via Agrobacterium tumefaciens. The coding sequence of avrGf1 was placed under the control of the constitutive CaMV 35S promoter in the binar...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-011-1045-7
更新日期:2011-07-01 00:00:00
abstract::Compact, friable and embryogenic calli were initiated from immature inflorescences and young leaf bases of one week old seedlings of Paspalum scrobiculatum cultured on MS medium supplemented with 2,4-D. A stable, embryogenic suspension culture was initiated from these calli and maintained in a liquid version of the sa...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00274134
更新日期:1989-05-01 00:00:00
abstract::DNA transfer into zygotic embryos of Eucalyptus globulus by microprojectile bombardment was studied with two devices: a gunpowder apparatus and a compressed-helium system. Using, as a test, the transient expression of a reporter gene, we optimized the physical and biological conditions of bombardment. Six-day-old cult...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232737
更新日期:1995-07-01 00:00:00
abstract::Programmed cell death (PCD) is a process that occurs both in animals and in plants and is an essential element in developmental processes. Pollination is a key factor in fruit production and self-incompatibility is one of the main limiting factors of this process. PCD has recently been put forward as a possible cause ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-010-0845-5
更新日期:2010-06-01 00:00:00
abstract:KEY MESSAGE:We developed a method for detection of mutants in a large number of plants, and found this method to be applicable to detection of a mutant allele at a concentration of 1/1000. Many techniques for SNP analysis have been developed, but most of these techniques are not so sensitive to be used for detection of...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-018-2274-9
更新日期:2018-06-01 00:00:00
abstract::Six sugarbeet (Beta vulgaris L.) lines (GWI-248, SPB-11, MonoHy 55, SMS-1, EL45 and FC607) were tested for regeneration. Shoot cultures were initiated in vitro from naked, sterilized embryos obtained from mature seed. Excised petioles from cultured shoots were plated on Gamborg's B5 medium and four modified Murashige ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00272972
更新日期:1988-01-01 00:00:00
abstract::Cotyledons from aseptically grown seedlings of sainfoin (Onobrychis viciifolia Scop.) were used as explant material to grow callus tissue for periods of 21 and 31 days. The formation of cells containing condensed tannins was induced by adding a range of BAP concentrations to an mB5 culture medium containing 2,4-D. Aft...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269314
更新日期:1988-05-01 00:00:00
abstract:KEY MESSAGE:An inorganic pyrophosphorylase gene, ThPP1 , modulated the accumulations of phosphate and osmolytes by up-regulating the differentially expression genes, thus enhancing the tolerance of the transgenic rice to alkali stress (AS). Inorganic pyrophosphorylase is essential in catalyzing the hydrolysis of pyroph...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-017-2208-y
更新日期:2017-12-01 00:00:00
abstract:: This study was conducted to detect the optimum conditions for DNA transfer into mature embryos of barley via electroporation. Cultured mature embryos of barley were directly electroporated in the presence of the pBI 121 vector carrying both the β-glucuronidase (GUS) and neomycin phosphotransferase II (npt II) genes. ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002999900182
更新日期:2000-07-01 00:00:00
abstract::A basic developmental framework of the Larix leptolepis Gord male gametophyte is presented in detail by squashing technique. The duration of the meiosis stage was more than 6 months, and included a long diffuse stage during winter. This long duration of the diffuse appearance of the diplotene stage makes L. leptolepis...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-008-0579-9
更新日期:2008-11-01 00:00:00
abstract::Most infections of plant roots are initiated in the region of elongation; the mechanism for this tissue-specific localization pattern is unknown. In alfalfa expressing PsUGT1 antisense mRNA under the control of the cauliflower mosaic virus (CaMV) 35S promoter, the cell cycle in roots is completed in 48 h instead of 24...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-004-0787-x
更新日期:2004-07-01 00:00:00
abstract::We developed an efficient plant regeneration system from protoplasts for poplar (Populus alba L.). Protoplasts were isolated from 4-day-old suspension cultures derived from seed-induced calli with a yield of 6.96× 106 cells/g fresh weight cells and then cultured at a concentration of 2.5×105 cells/ml in NH4NO3-free Mu...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050378
更新日期:1998-01-01 00:00:00
abstract::Stable transformation of papaya (Carica papaya L.) has been achieved following DNA delivery via high velocity microprojectiles. Three types of embryogenic tissues, including immature zygotic embryos, freshly explanted hypocotyl sections, and somatic embryos derived from both, were bombarded with tungsten particles car...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232177
更新日期:1990-08-01 00:00:00
abstract::A series of RAPD markers generated by a single 10-mer primer were analyzed by hybridization to amplified and genomic DNA and by sequencing in two Brassica species. Primer B18 produced different profiles of nine major bands each in both Brassica nigra (B genome) and B. napus (AC genomes). Cloning and sequencing of five...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232727
更新日期:1995-07-01 00:00:00
abstract::Autoclaving is a standard procedure for sterilizing nutrient media for plant tissue cultures. Most tissue cultures are grown at pH 5.2 to 5.8 with pH adjustments being made prior to autoclaving. This paper reports that there are significant differences between initial pH levels and pH levels following autoclaving, par...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269825
更新日期:1986-08-01 00:00:00
abstract::Quantitative trait loci (QTLs) associated with androgenic responsiveness in triticale were analyzed using a population of 90 DH lines derived from the F1 cross between inbred line 'Saka 3006' and cv. 'Modus', which was used in a number of earlier studies on molecular mapping in this crop. Using Windows QTL Cartographe...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-012-1320-2
更新日期:2012-11-01 00:00:00