Abstract:
:The expression and functionality of a resveratrol synthase (RS) gene from peanut (Arachis hypogaea) was studied using an Agrobacterium tumefaciens-mediated transient expression system in Nicotiana benthamiana leaves. Functional analysis of RS was demonstrated by tracking its expression during 96 h. To measure the transcripts levels of RS transgene, real-time qRT-PCR was used and revealed that the highest level of transcripts was at 48 h post-transfection. Western blot analyses showed that RS protein was accumulated to the highest levels at 72 h post-transfection. Finally, HPLC and mass spectrometry analyses revealed the production of trans-piceid (resveratrol glucoside) as the major stilbenoid compound confirming the functional activity of the RS enzyme in planta. No activity of RS transgene was detected in negative controls. This strategy showed advantages over conventional systems because it does not require establishment of cell cultures, feeding with appropriate substrates or generation of stable transgenic plants. This transient system proved to be a rapid and direct approach to perform functional analysis of stilbene synthases, such as resveratrol synthase. Furthermore, this approach can be useful to study the metabolic effects of over-expressing or silencing specific genes within a short period of time.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Condori J,Medrano G,Sivakumar G,Nair V,Cramer C,Medina-Bolivar Fdoi
10.1007/s00299-008-0664-0subject
Has Abstractpub_date
2009-04-01 00:00:00pages
589-99issue
4eissn
0721-7714issn
1432-203Xjournal_volume
28pub_type
杂志文章abstract::Medicago sativa lines with a high incidence of regeneration were established as suspension cultures and used to select for NaCl tolerant lines. Attempts were then made to regenerate plants from these lines. Regeneration was severely depressed in NaCl tolerant calli and the only plants that were successfully regenerate...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269335
更新日期:1983-06-01 00:00:00
abstract::A microscopical study was performed in order to localize chromogenic valepotriates in roots (in vivo) and in callus and root organ cultures (in vitro) of valerian plants. These in vitro cultures (producing valepotriates) contained lipid droplets having the same aspect as those containing essential oil described in the...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00270186
更新日期:1983-12-01 00:00:00
abstract::Recent developments in modern biotechnology such as the use of RNA interference (RNAi) have broadened the scope of crop genetic modification. RNAi strategies have led to significant achievements in crop protection against biotic and abiotic stresses, modification of plant traits, and yield improvement. As RNAi-derived...
journal_title:Plant cell reports
pub_type: 杂志文章,评审
doi:10.1007/s00299-019-02446-6
更新日期:2019-12-01 00:00:00
abstract::Phytohormones are essential signal compounds in the regulation of stress-related and defense-related genes. However, there is no clear evidence for any effect of these signal molecules and biotic elicitors on the regulation of the SALT gene in suspension-cultured rice cells. We characterized the expression of a SALT g...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-004-0836-5
更新日期:2004-10-01 00:00:00
abstract::Tree peony (Paeonia suffruticosa Andrews), a woody deciduous shrub, belongs to the section Moutan DC. in the genus of Paeonia of the Paeoniaceae family. To increase the efficiency of breeding, two EST-derived marker systems were developed based on a tree peony expressed sequence tag (EST) database. Using target region...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-011-1164-1
更新日期:2012-02-01 00:00:00
abstract:KEY MESSAGE:MANNANASE7 gene in Brassica napus L. encodes a hemicellulose which located at cell wall or extracellular space and dehiscence-resistance can be manipulated by altering the expression of MANNANASE7. Silique dehiscence is an important physiological process in plant reproductive development, but causes heavy y...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-020-02638-5
更新日期:2021-02-01 00:00:00
abstract::Shoot cultures ofAtractylodes lancea DC. (Compositae) have been established by inoculating the flower bud on Linsmaier-Skoog's medium supplemented with 10(-5) M naphthaleneacetic acid and 10(-5) M 6-benzyl-aminopurine. Shoots were multiplied on a medium containing 10(-6) M 6-benzylaminopurine. Propagated shoots rooted...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF02441005
更新日期:1984-06-01 00:00:00
abstract::The introgression of new desirable characters is very necessary in common bean (Phaseolus vulgaris L.)Interspecific hybridization of P. vulgaris L. with P. angustissimus A. Gray, a wild species with narrow leaves, could be successfully achieved for the first time using embryo culture. Sixteen to twenty-three-day old e...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00268593
更新日期:1986-10-01 00:00:00
abstract:KEY MESSAGE:An RNAseq-based analysis of the cassava plants inoculated with Xam allowed the identification of transcriptional upregulation of genes involved in jasmonate metabolism, phenylpropanoid biosynthesis and putative targets for a TALE. Cassava bacterial blight, a disease caused by the gram-negative bacterium Xan...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-014-1667-7
更新日期:2014-11-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-007-0321-z
更新日期:2007-07-01 00:00:00
abstract::Gastrodia elata Bl. is an achlorophyllous orchid plant feeding on the fungus Armillaria mellea. The plant lives underground during its life cycle except for florescence. Gastrodianins, members of the superfamily of monocot mannose-binding protein (MBP), have been identified from Gastrodia elata, yet their physiologica...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-006-0226-2
更新日期:2007-03-01 00:00:00
abstract::The effects of cytokinins on the different branches of the indole alkaloid pathway were investigated in Catharanthus roseus cell cultures. Addition of zeatin to a 2,4-dichlorophenoxyacetic acid-containing medium decreased tryptamine levels and increased the bioconversion of secologanin to ajmalicine. Zeatin also enhan...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00233425
更新日期:1993-10-01 00:00:00
abstract::A series of RAPD markers generated by a single 10-mer primer were analyzed by hybridization to amplified and genomic DNA and by sequencing in two Brassica species. Primer B18 produced different profiles of nine major bands each in both Brassica nigra (B genome) and B. napus (AC genomes). Cloning and sequencing of five...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232727
更新日期:1995-07-01 00:00:00
abstract::In a study of the 5'-flanking sequence of the Zea mays L. (maize) Glb1 gene in vitro, serial promoter deletions were generated and linked with the β-glucuronidase (GUS) reporter gene. The promoter deletion-GUS fusions were introduced into the maize P3377 cell line by particle bombardment. GUS assays indicated that tre...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050459
更新日期:1998-05-01 00:00:00
abstract::A protocol for Agrobacterium-mediated transformation with mannose selection was developed for cotyledon petiole, hypocotyl and leaf explants of tomato (Lycopersicon esculentum L. Mill). More than 400 transgenic plants from three tomato varieties were selected with 1% mannose in combination with 0.1-0.5% glucose. Avera...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-004-0809-8
更新日期:2004-10-01 00:00:00
abstract::Rice plants (Oryza sativa L., Chinsurah Boro II var. Indica) were regenerated from protoplasts isolated from microspore derived cell suspensions. A simple procedure for the establishment of such cell suspension cultures from embryogenic microcallus derived from cultured isolated microspores of Indica-type rice is desc...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232295
更新日期:1990-09-01 00:00:00
abstract::A simple method using molecular hybridization was devised to quantitatively measure chloroplast DNA synthesis in vivo. Total cellular DNA isolated from Nicotiana tabacum suspension cells, labeled with (3)H-thymidine, was hybridized to nitrocellulose membrane-bound cloned chloroplast DNA (ct DNA) fragments. Colorless, ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269202
更新日期:1985-04-01 00:00:00
abstract:KEY MESSAGE:The dynamic alterations of the physiological and molecular processes in reproductive stage soybean indicated the dramatic impact caused by drought. Drought is a major abiotic stress that limits soybean (Glycine max) production. Most prior studies were focused on either model species or crops that are at the...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-018-2332-3
更新日期:2018-12-01 00:00:00
abstract::Hypocotyl explants of three leguminous forest tree species, Albizia amara, A. lucida and A. richardiana, have differentiated shoot buds on B5 basal medium. Maximum number of shoots per explant developed on basal medium augmented with 2,4-D (0.1 μM) in A. amara (2) and BA (10 μM) for both A. lucida (2) and A. richardia...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269518
更新日期:1988-10-01 00:00:00
abstract::A collection of 29 pathogenesis-related 10 (PR10) genes of Medicago sativa and Medicago truncatula showed that they were almost all obtained from cDNA libraries of tissues undergoing abiotic or biotic stresses. The predicted proteins could be divided into two subclasses, PR10.1 and PR10.2, but in silico predicted mode...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-010-0909-6
更新日期:2010-11-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232412
更新日期:1992-12-01 00:00:00
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journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-016-1984-0
更新日期:2016-08-01 00:00:00
abstract::We have developed a plant- Escherichia coli pASV shuttle vector from the essential elements of the Ageratum yellow vein virus (AYVV). The geminivirus vector contains the AYVV genome with the coat-protein deletion, the E. coli vector backbone of pUC19, a unique cloning site and gene expression cassettes for plant selec...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-004-0792-0
更新日期:2004-08-01 00:00:00
abstract:: A system for barley transformation via polyethyleneglycol-mediated DNA uptake into protoplasts isolated directly from scutella and the regeneration of transgenic plants is reported. Scutellum protoplasts (cv. Clipper, an Australian malting cultivar) were co-transformed with plasmids Act 1-DGUS, containing the marker ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990000220
更新日期:2000-10-01 00:00:00
abstract::Seeds are heterogeneous storage reserves with wide array of storage compounds that include various soluble carbohydrates, starch polymer, storage proteins and lipids. These stored reserves comprise 70% of the world's caloric intake in the form of food and animal feed produced through sustainable agriculture, which con...
journal_title:Plant cell reports
pub_type: 社论
doi:10.1007/s00299-017-2135-y
更新日期:2017-05-01 00:00:00
abstract::The activity of a predicted promoter, PMC8, from Milk vetch dwarf virus was evaluated by comparing it with the cauliflower mosaic virus 35S RNA promoter (P35S) and PNCR, a promoter from Soybean chlorotic mottle virus. When the GUS fusion gene was introduced into tobacco, PMC8 showed a similar expression profile to P35...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-005-0917-0
更新日期:2005-06-01 00:00:00
abstract::The tobacco cell line TX1 (Nicotiana tabacum) accumulates up to 10 mg cinnamoyl putrescines/g dry weight. This level was further increased under various growth limiting conditions. The negative effect of accumulated phosphate on the optimal expression of cinnamoyl putrescine biosynthesis was prevented by the fedbatch ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00270221
更新日期:1984-02-01 00:00:00
abstract:: Cells of a suspension culture of Doritaenopsis cv. New Toyohashi were placed in a mixture of 2 M glycerol and 0.4 M sucrose for 15 min at room temperature and then dehydrated with a vitrification solution (PVS2) for 1-3 h on ice and plunged into liquid nitrogen. The highest viability (64% by 2,3,5-triphenyltetrazoliu...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990000255
更新日期:2000-12-01 00:00:00
abstract:KEY MESSAGE:Transcriptomes of two switchgrass genotypes representing the upland and lowland ecotypes will be key tools in switchgrass genome annotation and biotic and abiotic stress functional genomics. Switchgrass (Panicum virgatum L.) is an important bioenergy feedstock for cellulosic ethanol production. We report ge...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-016-2065-0
更新日期:2017-01-01 00:00:00
abstract::Plants have developed diverse molecular mechanisms to resist viruses. RNA silencing plays a dominant role in antiviral defense. Recent studies have correlated plant antiviral silencing to epigenetic modification in genomic DNA and protein by remodeling the expression levels of coding genes. The plant host methylation ...
journal_title:Plant cell reports
pub_type: 杂志文章,评审
doi:10.1007/s00299-019-02414-0
更新日期:2019-09-01 00:00:00