Abstract：
:Domain of Unknown Function 966 (DUF966) gene family was found in the protein family database, which consisted of seven genes in rice. The proteins encoded by these genes contained one or two highly conserved DUF966 domains. The available data of public microarray databases implied that these genes might play crucial roles in plant response to abiotic stresses. In this study, a member of the DUF966 gene family, DUF966-stress repressive gene 2 in Oryza sativa (OsDSR2, Loc_Os01g62200), was cloned and its role in rice responding to salt and simulated drought stresses was functionally characterized. OsDSR2 was expressed mainly in nodes of stems and leaf blades from rice. Expression profile analysis of adversity showed that OsDSR2 had different transcriptional responses to salt, drought, cold, heat and oxidative (H2O2) stresses, as well as abscisic acid (ABA), methyl jasmonate, salicylic acid, gibberellin acid and auxin treatments. Transient expression demonstrated that OsDSR2 was localized in the membrane and nucleus. Overexpression of OsDSR2 could increase salt and simulated drought (polyethyleneglycol)-stress sensitivities in rice by downregulating the expression of ABA- and stress-responsive genes including OsNCED4, SNAC1, OsbZIP23, P5CS, Oslea3 and rab16C. Furthermore, OsDSR2-overexpressing plants showed reduced ABA sensitivity during the post-germination stage. These results suggested that OsDSR2 negatively regulated rice response to salt and simulated drought stresses as well as ABA signaling, which provided some useful data for understanding the functional roles of DUF966 family genes in abiotic stress responses in plants.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Luo C,Guo C,Wang W,Wang L,Chen Ldoi
10.1007/s00299-013-1532-0subject
Has Abstractpub_date
2014-02-01 00:00:00pages
323-36issue
2eissn
0721-7714issn
1432-203Xjournal_volume
33pub_type
杂志文章abstract：:A cDNA clone encoding an ADP-ribosylation factor from potato (Solanum tuberosum L.) was isolated. The nucleotide and deduced amino acid sequences show high homology to known ADP-ribosylation factor sequences from Arabidopsis, yeast, cow and man. In northern blot experiments, all tissues analysed showed expression of t...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00233786
更新日期：1994-12-01 00:00:00
abstract：:Euphorbia dulcis endosperm is the site of controlled long lasting endocellular lysis involving segregation and autophagy of portions of the cytoplasm within the endoplasmic reticulum membrane. The lysis products may constitute a food source for the benefit of the early developing embryo. ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00231924
更新日期：1996-05-01 00:00:00
abstract：:A novel strategy for the production and maintenance of morphogenic callus for 1 year from mature leaf explants of apple has been developed using micropropagated primary leaves of cv. Golden Delicious. The technique required second generation adventitious buds produced from cultured primary leaves also produced from es...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990050446
更新日期：1998-04-01 00:00:00
abstract：:The gene coding for the green fluorescent protein (GFP) from jellyfish was introduced into conifer tissues by microprojectile bombardment and its transient expression was detected. Two versions of the GFP gene, wild-type GFP and modified GFP with a cryptic intron removed, were directly compared for their expression in...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF01088278
更新日期：1997-02-01 00:00:00
abstract：:Plant regeneration in Arachis pintoi was obtained via two developmental pathways: organogenesis and somatic embryogenesis. Organogenic callus cultures were initiated from pieces of leaf on MS medium supplemented with NAA or 2,4-D in combination with BA, KIN or 2iP. The most suitable combination for plant regeneration ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990000198
更新日期：2000-09-01 00:00:00
abstract：:Cell proliferation has been induced from the cambial zone of a branch of Acacia senegal, kept on the basal Knop and Ball medium in the presence of auxin. Transferred on the more complete nutrient medium of Schenk and Hildebrandt, the colonies gave rise to several cell lines. One of the friable lines, consisting of agg...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00268603
更新日期：1986-10-01 00:00:00
abstract：KEY MESSAGE:Rice and chickpea GDPD s are transcriptionally influenced by mineral deficiencies; especially, by phosphate starvation and CaGDP1 encodes an active glycerophosphodiester phosphodiesterase enzyme. Glycerophosphodiester phosphodiesterases (GDPDs) are enzymes involved in the degradation of glycerophosphodieste...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-016-1984-0
更新日期：2016-08-01 00:00:00
abstract：:A system to regenerate fertile rice (Oryza sativa L.) plants (both indica and japonica varieties) from protoplasts isolated from anther-derived embryogenic haploid suspension cultures has been established. Green plants were regenerated from protoplast-derived cell clusters five months after suspension culture initiati...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232422
更新日期：1992-12-01 00:00:00
abstract：:To develop an Agrobacterium mediated transformation protocol for chrysanthemum we studied the transformation efficiency of commonly used A.tumefaciens strains on 14 genotypes by comparing tumour size and frequency. One genotype was analyzed in detail using 14 strains of both A.tumefaciens and A.rhizogenes. Only a few ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232106
更新日期：1991-01-01 00:00:00
abstract：:We developed an efficient procedure for transformation and regeneration of L. esculentum cv. Moneymaker from cotyledon explants. The effect of two parameters on the transformation frequency was investigated in detail. The use of feeder layers during cocultivation proved to be critical. In addition, it was found that A...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232816
更新日期：1993-09-01 00:00:00
abstract：:Brassica juncea L. is a Zn/Cd accumulator. To determine the physiological basis of its metal accumulation phenotype, the functional properties and role of the metal efflux transporter BjCET2 were investigated using transgenic technology. Heterologous expression of BjCET2 in the double mutant yeast strain Deltazrc1Delt...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-009-0723-1
更新日期：2009-08-01 00:00:00
abstract：: Embryogenic nucellar callus cultures of different Citrus species and cultivars growing in hormone-free medium were transferred to medium containing either sucrose or glycerol as the only carbohydrate source. Glycerol has been reported to induce further development of Citrus somatic embryos, while in the presence of s...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990000280
更新日期：2001-01-01 00:00:00
abstract：:Arabinogalactan proteins (AGPs) are important proteoglycans regulating somatic embryogenesis in diverse plant species. Embryogenic cells of somatic embryos are covered by special extracellular cell wall layer called extracellular surface matrix network (ECMSN) at their early developmental stages. Here we show that hig...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-007-0429-1
更新日期：2008-02-01 00:00:00
abstract：: Rooted regenerated shoots obtained from leaf and nodal segments of Rosmarinus officinalis were grown on a basal nutrient medium for 9 weeks. The regenerants were shown by means of HPLC and mass spectrometry to contain carnosic acid, a diterpenoid with antioxidant and medicinal properties. Five-week-old nodular green ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990050763
更新日期：2000-04-01 00:00:00
abstract：:Diarrheal diseases caused by Vibrio cholerae and enterotoxigenic Escherichia coli (ETEC) are worldwide health problems that might be prevented with vaccines based on edible plants expressing the B subunit from either the cholera toxin (CTB) or the E. coli heat labile toxin (LTB). In this work we analyzed the immunity ...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-007-0439-z
更新日期：2008-01-01 00:00:00
abstract：:The promotive effect of ethylene inhibitors (Els), i.e. AgNO3 and aminoethoxyvinylglycine (AVG) on de novo shoot regeneration from cultured cotyledonary explants of Brassica campestris ssp. pekinensis cv. Shantung in relation to polyamines (PAs) was investigated. The endogenous levels of free putrescine and spermidine...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00232630
更新日期：1994-03-01 00:00:00
abstract：:Optimum conditions for the isolation and culture of protoplasts from high anthocyanin-producing callus of sweet potato (Ipomoea batatas) were investigated. Growth phase of callus and the ratio of callus-enzyme solution affected the yield of protoplasts. Composition of the medium and protoplast density were examined fo...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00269369
更新日期：1985-10-01 00:00:00
abstract：:Anthocyanin is the primary pigment contributing to red, violet, and blue flower color formation. The solubility of anthocyanins is enhanced by UDP glucose: flavonoid 3-O-glucosyltransferase (UFGT) through transfer of the glucosyl moiety from UDP-glucose to 3-hydroxyl group to produce the first stable pigments. To asse...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-011-1006-1
更新日期：2011-06-01 00:00:00
abstract：:A friable and fast-growing type of callus was isolated from a long term shoot-competent cell culture of wheat. The suspension cultures established from this callus consisted of small, densely cytoplasmic cells which divided more rapidly but with a lower plant regeneration frequency than the original culture. A high yi...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00231799
更新日期：1991-03-01 00:00:00
abstract：:Hairy root culture of Ginseng (Panax ginseng) was established after roots were induced on callus following infection with Agrobacterium rhizogenes. The transformed cultures of ginseng could be subcultured as an axenic root culture in the absence of phytohormones, and grew with extensive lateral branches more rapidly t...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00272780
更新日期：1987-12-01 00:00:00
abstract：:The parameters for optimal regeneration of seven commercial strawberry cultivars were tested using a range of explants and culture conditions. Efficient levels of regeneration--those needed to carry out transformation experiments--with the cultivars Calypso, Pegasus, Bolero, Tango and Emily were achieved with leaf dis...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-002-0530-4
更新日期：2003-01-01 00:00:00
abstract：:The effects of NaCl, feeder cells and the embedding of protoplasts in calcium alginate have been investigated in an attempt to improve culture conditions of recalcitrant sugar beet (Beta vulgaris L.) mesophyll protoplasts. While the use of NaCl in all instances proved detrimental to protoplast development, the other t...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00237431
更新日期：1993-04-01 00:00:00
abstract：:Embryogenic cell suspension cultures were established from Triticum aestivum X Leymus angustus F1 hybrids, using compact nodular calli derived from inflorescence segments. Calli originating from leaf segments did not give rise to stable cell suspensions. Growth measurements of the cell suspensions revealed that they c...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00235258
更新日期：1992-03-01 00:00:00
abstract：:An efficient method was developed using floating membrane rafts (Liferaft(™)) for the micropropagation of Aconitum napellus (Ranunculaceae), a cut flower crop with a low natural propagation rate. This was achieved by introducing shoot tips into culture on Murashige and Skoog's (1962) solid medium, or liquid medium-sup...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00238594
更新日期：1995-03-01 00:00:00
abstract：:The transit peptide sequence of ibAGP2 (TP2) was found to be capable of targeting protein into the chloroplast in the Arabidopsis protoplasts. TP2 was fused to a beta-glucuronidase (GUS) reporter gene and expressed in Arabidopsis under the control of the ibAGP2 promoter with the aim of dissecting the effect of the tra...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-008-0563-4
更新日期：2008-08-01 00:00:00
abstract：KEY MESSAGE:Marker-free transgenic plants can be generated with high efficiency by using the Cre/ lox P self-excision system controlled by the pollen- and embryo-specific Arabidopsis DLL promoter. In this work, we aimed to study the feasibility of using the pollen- and embryo-specific DLL promoter of the At4g16160 gene...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-014-1726-0
更新日期：2015-03-01 00:00:00
abstract：:A rapid micropropagation system was developed for Mucuna pruriens f. pruriens using explants from 1-week-old aseptically grown seedlings. Multiple shoot regeneration occurred following an initial callus growth on Revised Tobacco (RT) medium supplemented with 2.7 μM NAA and 9.8 μM 2iP. Maximum number of shoot regenerat...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00193734
更新日期：1995-12-01 00:00:00
abstract：:The in vivo nitrate reductase activity in 8 day old dark-grown sugarcane callus was over three fold that of the light-grown callus. NADH (0.3 mM) in the reaction system, increased the in vivo nitrate reductase activity by more than two fold both in the dark- and the light-grown callus tissues. The NADH dependence of n...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/BF00270207
更新日期：1984-08-01 00:00:00
abstract：KEY MESSAGE:A complete set of monosomic alien addition lines of Brassica napus with one of the seven chromosomes of Isatis indigotica and the recombinant mitochondria was developed and characterized. Monosomic alien addition lines (MAALs) are valuable for elucidating the genome structure and transferring the useful gen...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s00299-014-1621-8
更新日期：2014-08-01 00:00:00
abstract：:Leaf, rhizome, and stem explants of Helianthus smithii, a wild relative of the cultivated sunflower, have been tested for their morphogenic potential on media containing a range of hormonal combinations including α-naphthaleneacetic acid and 6-benzylaminopurine (0-2 mg/l). Somatic embryos appeared on the majority of t...
journal_title：Plant cell reports
pub_type： 杂志文章
doi：10.1007/s002990050303
更新日期：1997-07-01 00:00:00
