Genetic transformation of Chrysanthemum using wild type Agrobacterium strains; strain and cultivar specificity.

abstract：:This study aimed at establishing a protocol to increase the number of regenerated shoots and to limit the recovery of "escapes" during the regeneration of transgenic flax plants (cv Barbara). Here, we describe how light, adapted media and selection scheme could stimulate the transformation process, the organogenic pot...

journal_title：Plant cell reports

authors： Caillot S,Rosiau E,Laplace C,Thomasset B

更新日期：2009-03-01 00:00:00

abstract：KEY MESSAGE:Several hypomethylated sites within the Karma region of EgDEF1 and hotspot regions in chromosomes 1, 2, 3, and 5 may be associated with mantling. One of the main challenges faced by the oil palm industry is fruit abnormalities, such as the "mantled" phenotype that can lead to reduced yields. This clonal abn...

journal_title：Plant cell reports

authors： Sarpan N,Taranenko E,Ooi SE,Low EL,Espinoza A,Tatarinova TV,Ong-Abdullah M

更新日期：2020-09-01 00:00:00

abstract：:Quantitative real-time polymerase chain reaction (PCR) assays were designed that enabled the zygosity of transgenes in soybean [Glycine max (L.) Merrill] and peanut (Arachis hypogaea L.) to be determined. The two zygosity assays, based on TaqMan technology that uses a fluorogenic probe which hybridizes to a PCR target...

journal_title：Plant cell reports

authors： Schmidt M,Parrott W

更新日期：2001-07-01 00:00:00

abstract：:A selectable marker gene (SMG), usually conferring resistance to an antibiotic or herbicide, is generally introduced into the plant cells with the gene(s) for the trait of interest to allow only the cells that have integrated and express the foreign sequences to regenerate into a plant. The availability of several SMG...

journal_title：Plant cell reports

authors： Rosellini D,Capomaccio S,Ferradini N,Savo Sardaro ML,Nicolia A,Veronesi F

更新日期：2007-07-01 00:00:00

abstract：:The article 'Instability of endosperm development in amphiploids and their parental species in the genus Avena L.', written by Paulina Tomaszewska and Romuald Kosina, was originally published electronically on the publisher's internet portal on 22 May with an incorrect copyright line. As the article was due to be publ...

journal_title：Plant cell reports

authors： Tomaszewska P,Kosina R

更新日期：2019-01-01 00:00:00

abstract：:The variegated Saintpaulia cultivar Thamires (Saintpaulia sp.), which has pink petals with blue splotches, is generally maintained by leaf cuttings. In contrast, tissue culture-derived progeny of the cultivar showed not only a high percentage of mutants with solid-blue petals but also other solid-color variants, which...

journal_title：Plant cell reports

authors： Sato M,Kawabe T,Hosokawa M,Tatsuzawa F,Doi M

更新日期：2011-05-01 00:00:00

abstract：:Programmed cell death (PCD) is a process that occurs both in animals and in plants and is an essential element in developmental processes. Pollination is a key factor in fruit production and self-incompatibility is one of the main limiting factors of this process. PCD has recently been put forward as a possible cause ...

journal_title：Plant cell reports

authors： Serrano I,Pelliccione S,Olmedilla A

更新日期：2010-06-01 00:00:00

abstract：: An effective method has been developed for the stable transformation and regeneration of Cavendish banana (Musa spp. AAA group) cv 'Grand Nain' by microprojectile bombardment. Embryogenic cell suspensions were initiated using immature male flowers as the explant. Cells were co-bombarded with the neomycin phosphotrans...

journal_title：Plant cell reports

authors： Becker DK,Dugdale B,Smith MK,Harding RM,Dale JL

更新日期：2000-01-01 00:00:00

abstract：:Accurate and timely detection of transgene copy number in sugarcane is currently hampered by the requirement to use Southern blotting, needing relatively large amounts of genomic DNA and, therefore, the continued growth and maintenance of bulky plants in containment glasshouses. In addition, the sugarcane genome is bo...

journal_title：Plant cell reports

authors： Casu RE,Selivanova A,Perroux JM

更新日期：2012-01-01 00:00:00

abstract：:Cell suspension cultures of cowpea (Vigna sp.) were able to produce extracellular peroxidase. Different growth regulator concentrations induced different peroxidase activity in callus. The crude extracellular medium after four weeks of culture showed higher (6 times) specific peroxidase activity and higher thermo stab...

journal_title：Plant cell reports

authors： Moreno OA,Vazquez-Duhalt R,Nolasco H

更新日期：1990-07-01 00:00:00

abstract：KEY MESSAGE:Oral administration of maize-expressed H3N2 nucleoprotein induced antibody responses in mice showing the immunogenicity of plant-derived antigen and its potential to be utilized as a universal flu vaccine. Influenza A viruses cause influenza epidemics that are devastating to humans and livestock. The vaccin...

journal_title：Plant cell reports

authors： Nahampun HN,Bosworth B,Cunnick J,Mogler M,Wang K

更新日期：2015-06-01 00:00:00

abstract：:The non-yellowing gene (NYE1), initially identified from Arabidopsis, is a key regulatory gene responsible for chlorophyll degradation during senescence. Here, FaNYE1, an orthologue of AtNYE1, was further identified from a major type of cool-season turf grass, tall fescue (Festuca arundinacea Schreb.), by RACE-PCR. It...

journal_title：Plant cell reports

authors： Wei Q,Guo Y,Kuai B

更新日期：2011-07-01 00:00:00

abstract：:We have previously developed procedures for the efficient production of sweet orange (Citrus sinensis L. Osbeck) and Carrizo citrange (C. sinensis L. Osbeck×Poncirus trifoliata L. Raf.) transgenic plants using an Agrobacterium tumefaciens-mediated transformation and shoot tip grafting in vitro regeneration system. We ...

journal_title：Plant cell reports

authors： Peña L,Cervera M,Juárez J,Navarro A,Pina JA,Navarro L

更新日期：1997-09-01 00:00:00

abstract：:An improved protocol forAgrobacterium-mediated transformation of the tomato cultivar Moneymaker was developed by examining the effects of six different factors on the efficiency of transformation. Explant size, explant orientation, gelling agent and plate sealant were found to affect transformation efficiency. Two oth...

journal_title：Plant cell reports

authors： Frary A,Earle ED

更新日期：1996-12-01 00:00:00

abstract：:Randomly amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers were used to analyse the genetic diversity of Portuguese Prunus dulcis cultivars and their relationship to important foreign cultivars. Of the primers tested, 6 (out of 60) RAPD and 5 (out of 18) ISSR primers were selected for th...

journal_title：Plant cell reports

authors： Martins M,Tenreiro R,Oliveira MM

更新日期：2003-08-01 00:00:00

abstract：KEY MESSAGE:MicroRNAs have higher expression stability than protein-coding genes in B. napus seeds and are therefore good reference genes for miRNA and mRNA RT-qPCR analysis. Reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) has become the "gold standard" to gain insight into function of ...

journal_title：Plant cell reports

authors： Machado RD,Christoff AP,Loss-Morais G,Margis-Pinheiro M,Margis R,Körbes AP

更新日期：2015-07-01 00:00:00

abstract：:Hypocotyl explants of 1 and 10 mm lengths were excised from 12-day-old in vitro-grown seedlings of Albizia richardiana. The larger pieces, after 40 days of culture, developed shoots along with green calli on B5 + BAP (10(-7)-10(-5)M), while the smaller segments produced only green calli on B5+BAP (10(-7)-10(-4)M) medi...

journal_title：Plant cell reports

authors： Tomar UK,Gupta SC

更新日期：1988-01-01 00:00:00

abstract：KEY MESSAGE:Phytophthora infestans resistant somatic hybrids of S. × michoacanum (+) S. tuberosum and autofused 4 x S. × michoacanum were obtained. Our material is promising to introgress resistance from S. × michoacanum into cultivated potato background. Solanum × michoacanum (Bitter.) Rydb. (mch) is a wild diploid (2...

journal_title：Plant cell reports

authors： Smyda P,Jakuczun H,Dębski K,Sliwka J,Thieme R,Nachtigall M,Wasilewicz-Flis I,Zimnoch-Guzowska E

更新日期：2013-08-01 00:00:00

abstract：:Callus cultures from cotyledon explants were established and maintained in culture for more than two years. After several months callus cultures were transferred into liquid medium and cultured as cell suspensions. Protoplasts were isolated from these cell suspension cultures and cultured in a liquid medium. After for...

journal_title：Plant cell reports

authors： Koblitz H,Koblitz D

更新日期：1982-06-01 00:00:00

abstract：:The direct induction of adventitious shoots from leaf explants obtained from adult plants of Zhanhua winter jujube, an elite variety of Zizyphus jujuba Mill., is reported. The percentage of leaf explants producing shoots and the average number of shoots per explant were significantly improved when 10-day-old leaves we...

journal_title：Plant cell reports

authors： Gu XF,Zhang JR

更新日期：2005-03-01 00:00:00

abstract：KEY MESSAGE:The Arabidopsis AP2/ERF family transcription factor AtERF15 is nuclear-localized and positively regulates ABA and stress responses. Abscisic acid (ABA) is a major plant hormone that controls the expression of hundreds genes involved in various aspects of plant growth and development, such as seed developmen...

journal_title：Plant cell reports

authors： Lee SB,Lee SJ,Kim SY

更新日期：2015-01-01 00:00:00

abstract：:A genetic test was performed on seeds from 283 transgenic tobacco plants obtained by T-DNA transformation. Seeds from self-fertilized transgenic plants were germinated on kanamycin-containing medium, and the percentage of seeds which germinated, as well as the ratio of kanamycin-resistant to kanamycin-sensitive seedli...

journal_title：Plant cell reports

authors： Heberle-Bors E,Charvat B,Thompson D,Schernthaner JP,Barta A,Matzke AJ,Matzke MA

更新日期：1988-12-01 00:00:00

abstract：:Shoot and leaf segments of a non-regenerable Medicago sativa L. genotype were cocultivated with the "shooty" mutant of Agrobacterium tumefaciens carrying the pGV 2206 plasmid. Transformed callus lines were selected and regenerated on the hormone free B5 medium. Southern blot analysis demonstrated integration of T-DNA ...

journal_title：Plant cell reports

authors： Kuchuk N,Komarnitski I,Shakhovsky A,Gleba Y

更新日期：1990-04-01 00:00:00

abstract：:Site-specific recombination has been developed into a genetic engineering tool for higher eukaryotes. The manipulation of newly introduced DNA is now possible in the course of genetic transformation procedures, thus making the process more predictable and reliable. Also, a wide variety of chromosomal rearrangements us...

journal_title：Plant cell reports

authors： Lyznik LA,Gordon-Kamm WJ,Tao Y

更新日期：2003-06-01 00:00:00

abstract：:Expiants of five plant species (Allium cepa, Antirrhinum majus, Brassica campestris. Glycine max, and Nicotiana tabacum) were co-cultivated with three Agrobacterium tumefaciens strains under different conditions to assess the effects of acetosyringone and medium pH on strain virulence. Tumours were incited on all dico...

journal_title：Plant cell reports

authors： Godwin I,Todd G,Ford-Lloyd B,Newbury HJ

更新日期：1991-04-01 00:00:00

abstract：:Mimosa tenuiflora (Willd.) Poiret (Leguminosae) was micropropagated throughin vitro culture of axillary buds on Murashige and Skoog (MS) medium. Shoot formation was achieved when the media were supplemented with 0.1 mg.L(-1) IAA + 3 mg.L(-1) KN.In vitro rooting of regenerated shoots was achieved when 0.1 mg.L(-1) KN w...

journal_title：Plant cell reports

authors： Villarreal ML,Rojas G

更新日期：1996-11-01 00:00:00

abstract：KEY MESSAGE:This study established an efficient method of regenerating plants of Ficus lyrata and producing purple-leaved F. lyrata plants through genetic transformation using a VvMybA1 gene of grapevine. ABSTRACT:Ficus lyrata, a species with unique violin- or guitar-shaped leaves, was regenerated from leaf-derived ca...

journal_title：Plant cell reports

authors： Zhao J,Li ZT,Chen J,Henny RJ,Gray DJ,Chen J

更新日期：2013-11-01 00:00:00

abstract：:The influence of light on the expression of NADPH: protochlorophyllide oxidoreductase has been studied in different plant species. The presumptive precursors to this enzyme have been characterized by in vitro translation of poly (A) RNA and immunoprecipitation. Two bands of apparent molecular weights of about 42 000 a...

journal_title：Plant cell reports

authors： Meyer G,Bliedung H,Kloppstech K

更新日期：1983-02-01 00:00:00

abstract：:Four diverse strains of Agrobacterium tumefaciens (C58, Ach5, GV3111, and A281) were capable of inducing tumors at a high frequency on inoculated stems of lentil (Lens culinaris Medik. cultivar Laird) in vivo, and on excised shoot apices in vitro. GV3111 and Ach5 produced the largest and heaviest tumors in vivo, while...

journal_title：Plant cell reports

authors： Warkentin TD,McHughen A

更新日期：1991-12-01 00:00:00

abstract：:Diarrheal diseases caused by Vibrio cholerae and enterotoxigenic Escherichia coli (ETEC) are worldwide health problems that might be prevented with vaccines based on edible plants expressing the B subunit from either the cholera toxin (CTB) or the E. coli heat labile toxin (LTB). In this work we analyzed the immunity ...

journal_title：Plant cell reports

authors： Rosales-Mendoza S,Soria-Guerra RE,López-Revilla R,Moreno-Fierros L,Alpuche-Solís AG

更新日期：2008-01-01 00:00:00