Abstract:
:Callus cultures from cotyledon explants were established and maintained in culture for more than two years. After several months callus cultures were transferred into liquid medium and cultured as cell suspensions. Protoplasts were isolated from these cell suspension cultures and cultured in a liquid medium. After formation of new cell walls the cells were further cultured in liquid medium and afterwards transferred to an agar-solidified medium to give a vigorously growing callus culture. In the case of the cultivar 'Lukullus' shoots were recovered from callus. All efforts to root these shoots failed and this, in addition to variations in appearence, suggests that the shoots are changed genetically possibly due to the prolonged culture period.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Koblitz H,Koblitz Ddoi
10.1007/BF00269184subject
Has Abstractpub_date
1982-06-01 00:00:00pages
147-50issue
4eissn
0721-7714issn
1432-203Xjournal_volume
1pub_type
杂志文章abstract::Embryonal-suspensor tissue (EST) of Mediterranean cypress (Cupressus sempervirens L.) was tested for microprojectile-DNA delivery (by the PDS-1000/He device) for different subculture periods (9, 15, and 21 days) using the plasmid vectors pRT99GUS [containing the β-glucuronidase (GUS) and neomycin phosphotransferase (N...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050556
更新日期:1998-12-01 00:00:00
abstract:: A high frequency of embryogenesis and transformation from all parts of flowers of two lines of Medicago truncatula R-108-1 and Jemalong J5 were obtained. Using this flower system, we obtained transgenic plants expressing promoter-uidA gene fusions as well as the gfp living cell color reporter gene. Moreover, this met...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002999900168
更新日期:2000-06-01 00:00:00
abstract::The effect of the oxygen concentration in the aeration gas on regeneration from rice cells in bioreactor cultures was investigated. The efficiency of regeneration in cultures aerated with over 40% oxygen was higher than that in a flask culture. In the case of a culture in which the dissolved oxygen(DO) was saturated b...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232217
更新日期:1996-06-01 00:00:00
abstract::Two clones of Populus nigra L. were tested in vivo and in vitro for their susceptibility to three different Agrobacterium tumefaciens wild-type strains evaluating number and size of resulting calluses. Strain C58 proved to be the most virulent.Various parameters affecting Agrobacterium-mediated transformation of P. ni...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00233315
更新日期:1994-02-01 00:00:00
abstract::DNA transfer into zygotic embryos of Eucalyptus globulus by microprojectile bombardment was studied with two devices: a gunpowder apparatus and a compressed-helium system. Using, as a test, the transient expression of a reporter gene, we optimized the physical and biological conditions of bombardment. Six-day-old cult...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232737
更新日期:1995-07-01 00:00:00
abstract::A reliable plant regeneration system is described for the production of adventitious shoots from root explants of spinach. Explants from roots of axenic shoots and roots induced on cultured hypocotyl explants were used for adventitious shoot induction. Explants from apical, middle and basal root regions were incubated...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050359
更新日期:1997-12-01 00:00:00
abstract::The sugarcane (Saccharum officinarum) stress-related MYB transcription factor gene, ScMYBAS1, demonstrated induced response to water deficit and salt stress in our previous study. To elucidate its stress tolerance mechanism at the transcriptional level, we isolated and characterized the promoter (PScMYBAS1, 1,033 bp) ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-011-1183-y
更新日期:2012-04-01 00:00:00
abstract::A novel member of the AP2/ERF transcription factor family, SlERF5, was identified from a tomato mature leaf cDNA library screen. The complete DNA sequence of SlERF5 encodes a putative 244-amino acid DNA-binding protein which most likely acts as a transcriptional regulator and is a member of the ethylene responsive fac...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-011-1170-3
更新日期:2012-02-01 00:00:00
abstract::Protoplasts derived from oat (Avena sativa L.) suspension culture cells (7 days after subculturing) were electroporated with plasmid DNA containing the Escherichia coli uidA gene encoding the ß-glucuronidase reporter enzyme. Consistently high enzyme activity was observed with electroporation conditions of 500 μF and 1...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00234710
更新日期:1993-05-01 00:00:00
abstract:KEY MESSAGE:Overexpression of Withania somnifera SGT gene (WssgtL3.1) in transgenic Arabidopsis improves various agronomic and physiological traits and alters conjugated sterol levels to mitigate the effect of salt stress. Sterols are essential constituents of cell membranes that are involved in several biological func...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-021-02666-9
更新日期:2021-02-01 00:00:00
abstract::The influence of light on the expression of NADPH: protochlorophyllide oxidoreductase has been studied in different plant species. The presumptive precursors to this enzyme have been characterized by in vitro translation of poly (A) RNA and immunoprecipitation. Two bands of apparent molecular weights of about 42 000 a...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269229
更新日期:1983-02-01 00:00:00
abstract::Separation of stem proteins by sodium dodecyl sulfate polyacrylamide gel electrophoresis permitted classification of 17 celery varieties (Apium graveolens L.) into 7 groups. Several of these groups could be further subdivided into 11 subgroups with gel electrophoresis patterns of 4 isozyme systems. Classification from...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00276666
更新日期:1987-04-01 00:00:00
abstract::The sequences of the 3' untranslated region (UTR) of the manganese superoxide dismutase (MnSOD) genes in wheat (Triticum aestivum) were found to be quite variable with different predicted thermostabilities. The degradation rates of the 3' UTR variants and the coding region were measured following exposure to endogenou...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-005-0046-9
更新日期:2006-03-01 00:00:00
abstract:KEY MESSAGE:MsCBL4 expression in tobacco enhanced its salt and saline-alkali stress tolerance by regulating calcium accumulation in roots, indicating the important role of calcium metabolism in plant saline-alkali stress tolerance The calcineurin B-like (CBL) family of proteins play important roles in plant abiotic str...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-020-02543-x
更新日期:2020-08-01 00:00:00
abstract::A T-DNA insertional mutant OsPE of rice gives twin and triplet seedlings in up to 20 % of the seeds. Detailed cytological and histological analysis of OsPE indicated normal male and female gametogenesis in the OsPE mutant. Confocal laser scanning microscopic (CLSM) analysis of the developing seeds of OsPE showed multi...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-012-1291-3
更新日期:2012-10-01 00:00:00
abstract::A novel strategy for the production and maintenance of morphogenic callus for 1 year from mature leaf explants of apple has been developed using micropropagated primary leaves of cv. Golden Delicious. The technique required second generation adventitious buds produced from cultured primary leaves also produced from es...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050446
更新日期:1998-04-01 00:00:00
abstract:KEY MESSAGE:The recovery of non-functional-enhanced green fluorescence protein can be used as indicator to facilitate the identification of mutants generated by CRISPR/Cas9. The CRISPR/Cas9 system is a powerful tool for genome editing and it has been employed to knock out genes of interest in multiple plant species. Id...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-019-02465-3
更新日期:2019-12-01 00:00:00
abstract::The non-yellowing gene (NYE1), initially identified from Arabidopsis, is a key regulatory gene responsible for chlorophyll degradation during senescence. Here, FaNYE1, an orthologue of AtNYE1, was further identified from a major type of cool-season turf grass, tall fescue (Festuca arundinacea Schreb.), by RACE-PCR. It...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-011-1028-8
更新日期:2011-07-01 00:00:00
abstract:KEY MESSAGE:Galactoglucomannan oligosaccharides seem to interact with auxin in xylogenic cell culture, thus influencing mainly metaxylem-like tracheary element differentiation depending on timing with hormones and the process kinetics. Complex mapping of Zinnia mesophyll cell transdifferentiation into tracheary element...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-012-1379-9
更新日期:2013-04-01 00:00:00
abstract:KEY MESSAGE:Our data present H 2 S in a new role, serving as a multi-faceted transducer to different response mechanisms during NO-induced acquisition of tolerance to flooding-induced hypoxia in maize seedling roots. Nitric oxide (NO), serving as a secondary messenger, modulates physiological processes in plants. Recen...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-016-2037-4
更新日期:2016-11-01 00:00:00
abstract::The full-length cDNAs of eight S ribonucleases (S-RNases) were cloned from stylar RNA of European pear cultivars that could not be characterized by the cleaved amplified polymorphic sequences (CAPS) marker system for genotyping European pear cultivars harboring nine S alleles Sa, Sb, Sd, Se, Sh, Sk, Sl, Sq, and Sr. Co...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-006-0254-y
更新日期:2007-03-01 00:00:00
abstract::Phytohormones are essential signal compounds in the regulation of stress-related and defense-related genes. However, there is no clear evidence for any effect of these signal molecules and biotic elicitors on the regulation of the SALT gene in suspension-cultured rice cells. We characterized the expression of a SALT g...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-004-0836-5
更新日期:2004-10-01 00:00:00
abstract::The tobacco cell line TX1 (Nicotiana tabacum) accumulates up to 10 mg cinnamoyl putrescines/g dry weight. This level was further increased under various growth limiting conditions. The negative effect of accumulated phosphate on the optimal expression of cinnamoyl putrescine biosynthesis was prevented by the fedbatch ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00270221
更新日期:1984-02-01 00:00:00
abstract::Fully fertile plants with the expected chromosome number 2n=40 were regenerated from excised leaf sections of Glycine clandestina. Shoots formed directly on the explants through organogenesis. Utilizing the same media and procedures fully fertile plants were also regenerated from cotyledon and hypocotyl tissue of the ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269116
更新日期:1986-06-01 00:00:00
abstract::The effect of various inhibitors on differentiation (shoot morphogenesis) in callus cultures of Brassica, Datura and Nicotiana has been investigated. Hormone medium without any inhibitor (control), resulted in 6% shoot formation. Addition of inhibitors such as actinomycin D, cordycepin, abscisic acid, trigonelline and...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00270062
更新日期:1990-03-01 00:00:00
abstract::Treatment of Allium cepa L. cellsuspension cultures with a biotic elicitor derived from the fungus Botrytis cinerea, resulted in phytoalexin synthesis. Two phytoalexins, 5-octylcyclopenta-1,3-dione and 5-hexyl-cyclopenta-1,3-dione, were accumulated in cultured onion cells. Removal of extracellular Ca(2+) by the calciu...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00231593
更新日期:1996-09-01 00:00:00
abstract::An Arabidopsis mutant line T90, exhibiting a stem-specific and wound-responsive GUS expression was identified from a population of Arabidopsis thaliana tagged with a promoterless β-glucuronidase (GUS) in the T-DNA. Sequence flanking the insertion from the right border was amplified by TAIL PCR and cloned. The insertio...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-011-1205-9
更新日期:2012-05-01 00:00:00
abstract::Agrobacterium-mediated transformation frequency is very low with cells from some species such as soybeans. Studies were conducted to investigate the Agrobacterium-mediated transformation frequency in near-isogenic lipoxygenase mutant lines of soybeans, since the nigh level of lipoxygenase activity in soybean embryos m...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00231809
更新日期:1991-03-01 00:00:00
abstract::Rice plants (Oryza sativa L., Chinsurah Boro II var. Indica) were regenerated from protoplasts isolated from microspore derived cell suspensions. A simple procedure for the establishment of such cell suspension cultures from embryogenic microcallus derived from cultured isolated microspores of Indica-type rice is desc...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232295
更新日期:1990-09-01 00:00:00
abstract:KEY MESSAGE:An antigenic protein targeting two epitopes from the Zaire ebolavirus GP1 protein was expressed in plant cells rendering an antigen capable of inducing humoral responses in mouse when administered subcutaneously or orally. The 2014 Ebola outbreak made clear that new treatments and prophylactic strategies to...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-016-2088-6
更新日期:2017-02-01 00:00:00