Development of multiple embryos in polyembryonic insertional mutant OsPE of rice.

Abstract:

:A T-DNA insertional mutant OsPE of rice gives twin and triplet seedlings in up to 20 % of the seeds. Detailed cytological and histological analysis of OsPE indicated normal male and female gametogenesis in the OsPE mutant. Confocal laser scanning microscopic (CLSM) analysis of the developing seeds of OsPE showed multiple embryo development in up to 60 % of the ovules. The multiple embryos, mostly twins and triplets, and rarely quadruplets, developed through sequential cleavage from a single zygotic embryo in each ovule. The reduced number of multiple seedlings compared with multiple embryos observed in CLSM study may be attributed to their inability to develop further due to competition in a single embryo sac. Key message Multiple seedlings in the OsPE mutant are due to sequential proliferation and cleavage of the zygotic embryos. The nucellar tissue was not involved in multiple embryo development.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Paul P,Awasthi A,Kumar S,Verma SK,Prasad R,Dhaliwal HS

doi

10.1007/s00299-012-1291-3

subject

Has Abstract

pub_date

2012-10-01 00:00:00

pages

1779-87

issue

10

eissn

0721-7714

issn

1432-203X

journal_volume

31

pub_type

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