Abstract:
:An efficient micropropagation system for Saussurea involucrata Kar. et Kir., an endangered Chinese medicinal plant, has been developed. Shoot organogenesis occurred from S. involucrata leaf explants inoculated on medium with appropriate supplements of plant growth regulators. 66.0% of shoot regeneration frequency and 5.2 shoots per leaf explant were achieved when cultured on a medium containing 10 microM 6-benzylaminopurine (BAP) and 2.5 microM 1-naphthaleneacetic acid (NAA). Shoot organogenesis was improved further when the leaf explants were pre-incubated at low temperature, and 80.6% of shoot regeneration frequency was recorded with 9.3 shoots per leaf explant at 4 degrees C by 5-day pretreatment period. Up to 87.0% of the regenerated shoots formed complete plantlets on a medium containing 2.5 microM indole-3-acetic acid (IAA) within 28 days, and 85.2% of the regenerated plantlets survived and grew vigorously in greenhouse condition. The phytochemical profile of the micropropagated plants was similar to that of wild plants. The regeneration protocol developed in this study provides a basis for germplasm conservation and for further investigation of medicinally active constituents of the elite medicinal plant.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Guo B,Gao M,Liu CZdoi
10.1007/s00299-006-0230-6subject
Has Abstractpub_date
2007-03-01 00:00:00pages
261-5issue
3eissn
0721-7714issn
1432-203Xjournal_volume
26pub_type
杂志文章abstract::Murashige and Skoog's modified medium in 1% Difco Bacto-agar supplemented with sugar alcohols (sorbsitol, mannitol), growth regulators (1-naphthalenacetic acid, 2,4-dichlorophenoxyacetic acid, benzyladenine, kinetin) and sugars (fructose, glucose, sucrose) induced aposporous gametophytes from pinnae of Pteris vittata ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232608
更新日期:1991-10-01 00:00:00
abstract::Transgenic calli of Pharbitis nil which grow in the presence of kanamycin were obtained by introduction of plasmid pBI121, which carries kanamycin resistance and the gene for beta-glucuronidase. The calli were shown to have fragments of vector DNA in their genome and high levels of beta-glucuronidase activity. This is...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00274124
更新日期:1989-05-01 00:00:00
abstract::DNA fingerprints generated by the Jeffreys' probes, 33.6 and 33.15, indicated the presence of minisatellite-like sequences in the red clover genome. The fingerprints generated by probe 33.6 gave less background and fewer but better defined bands than those obtained with probe 33.15. Assay of a regenerative somaclonal ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00235293
更新日期:1993-12-01 00:00:00
abstract::LITHOSPERMUM ERYTHRORHIZON: , which are capable of producing red pigments, have been established. The red pigments were formed on the stems of L. erythrorhizon shoots cultured both on solid and in liquid media without phytohormones at 25 °C in the dark. Thin-layer chromatography, high-performance liquid chromatography...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990000237
更新日期:2000-11-01 00:00:00
abstract::Carotenoid cleavage dioxygenases (CCDs) are involved in the production of diverse apocarotenoids including phytohormones, the visual molecules and the aromatic volatile compounds derived from carotenoids. Here, we examined the spatial expression of four of the CCD genes (AtCcd1, 4, 7 and 8) among the nine members of t...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-010-0999-1
更新日期:2011-06-01 00:00:00
abstract::A method for regenerating pigeonpea [Cajanus cajan (L.) Millsp.] plants has been developed using distal cotyledonary segments of mature seeds as explants. A large number of shoot buds were induced directly from explants of genotypes T-15-15 and GAUT-82-90 when cultured on six different basal media fortified with 22.2 ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050469
更新日期:1998-06-01 00:00:00
abstract::Taro (Colocasia esculenta) is one of the most important crops in the Pacific Islands, however, taro yields have been declining in Hawaii over the past 30 years partly due to diseases caused by oomycete and fungal pathogens. In this study, an efficient Agrobacterium tumefaciens-mediated transformation method for taro i...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-008-0519-8
更新日期:2008-05-01 00:00:00
abstract::Somatic embryogenesis was induced in callus tissues derived from young flower buds ofPanax notoginseng via callus within 18 weeks of culture. The mature somatic embryos were germinated on half-strength Murashige and Skoog's (MS) medium supplemented with gibberellic acid A3(GA) and 6-benzyladenine (BA). The most suitab...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF01092764
更新日期:1997-04-01 00:00:00
abstract::Tree peony (Paeonia suffruticosa Andrews), a woody deciduous shrub, belongs to the section Moutan DC. in the genus of Paeonia of the Paeoniaceae family. To increase the efficiency of breeding, two EST-derived marker systems were developed based on a tree peony expressed sequence tag (EST) database. Using target region...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-011-1164-1
更新日期:2012-02-01 00:00:00
abstract::Protoplasts of sugarbeet (Beta vulgaris L.) were isolated from cell suspension cultures and cultured in modified PGo medium. Conditions required for the efficient division of the protoplasts were investigated.The optimal combination of phytohormones was found to be 1 mg/l NAA, 0.2 mg/l 2,4-D, 0.5 mg/l zeatin. Protopla...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269287
更新日期:1985-08-01 00:00:00
abstract:KEY MESSAGE:The T.118 and T.406 seedlings showed strong adaptability under Cd concentrations ≤ 50 µM. The mechanisms of photoprotection in T.118 and T.406 differed in high-Cd concentrations. To explore the physiological response characteristics of Taxodium hybrids to cadmium (Cd) stress and provide basis for screening ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-018-2327-0
更新日期:2018-11-01 00:00:00
abstract::Leaf, rhizome, and stem explants of Helianthus smithii, a wild relative of the cultivated sunflower, have been tested for their morphogenic potential on media containing a range of hormonal combinations including α-naphthaleneacetic acid and 6-benzylaminopurine (0-2 mg/l). Somatic embryos appeared on the majority of t...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050303
更新日期:1997-07-01 00:00:00
abstract:KEY MESSAGE:MANNANASE7 gene in Brassica napus L. encodes a hemicellulose which located at cell wall or extracellular space and dehiscence-resistance can be manipulated by altering the expression of MANNANASE7. Silique dehiscence is an important physiological process in plant reproductive development, but causes heavy y...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-020-02638-5
更新日期:2021-02-01 00:00:00
abstract::Twenty five clones from a carrot genomic library were used as probes to detect variations in the 5-methyl-cytosine pattern during somatic embryogenesis. The majority of them evidenced an invariant pattern. With two clones however, differences were found between the adulttype DNA pattern and the embryonic one. The leve...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00272097
更新日期:1990-05-01 00:00:00
abstract::The effect of the oxygen concentration in the aeration gas on regeneration from rice cells in bioreactor cultures was investigated. The efficiency of regeneration in cultures aerated with over 40% oxygen was higher than that in a flask culture. In the case of a culture in which the dissolved oxygen(DO) was saturated b...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232217
更新日期:1996-06-01 00:00:00
abstract:: Somatic embryogenesis and whole plant regeneration were achieved in callus cultures derived from immature zygotic embryos of Acacia mangium. Embryogenic callus was induced on MS medium containing combinations of TDZ (1-2 mg/l), IAA (0.25-2 mg/l) and a mixture of amino acids. Globular embryos developed on embryogenic ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990000288
更新日期:2001-01-01 00:00:00
abstract::To develop an alternative genetic transformation system that is not dependent on an antibiotic selection strategy, the phosphomannose isomerase gene (pmi) system was evaluated for producing transgenic plants of chickpea (Cicer arietinum L.). A shoot morphogenesis protocol based on the thidiazuron (TDZ)-induced shoot m...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-009-0766-3
更新日期:2009-11-01 00:00:00
abstract::Eustoma grandiflorum Shinn requires vernalization for the induction of stem elongation and flowering. To investigate the role of gibberellins (GAs) in vernalization, the expression levels of genes encoding enzymes of GA biosynthesis, copalyl diphosphate synthetase, GA 20-oxidase and GA 3 beta-hydroxylase, were examine...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-003-0672-z
更新日期:2003-09-01 00:00:00
abstract:KEY MESSAGE:Rice and chickpea GDPD s are transcriptionally influenced by mineral deficiencies; especially, by phosphate starvation and CaGDP1 encodes an active glycerophosphodiester phosphodiesterase enzyme. Glycerophosphodiester phosphodiesterases (GDPDs) are enzymes involved in the degradation of glycerophosphodieste...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-016-1984-0
更新日期:2016-08-01 00:00:00
abstract::Six sugarbeet (Beta vulgaris L.) lines (GWI-248, SPB-11, MonoHy 55, SMS-1, EL45 and FC607) were tested for regeneration. Shoot cultures were initiated in vitro from naked, sterilized embryos obtained from mature seed. Excised petioles from cultured shoots were plated on Gamborg's B5 medium and four modified Murashige ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00272972
更新日期:1988-01-01 00:00:00
abstract:: A high frequency of embryogenesis and transformation from all parts of flowers of two lines of Medicago truncatula R-108-1 and Jemalong J5 were obtained. Using this flower system, we obtained transgenic plants expressing promoter-uidA gene fusions as well as the gfp living cell color reporter gene. Moreover, this met...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002999900168
更新日期:2000-06-01 00:00:00
abstract::In this study, we report the obtaining of carrot plants expressing human interferon alpha-2b via Agrobacterium-mediated transformation using two vector constructs containing the sequence coding for interferon gene fused with Nicotiana plumbagenifolia calreticulin apoplast targeting signal driven by 35S CaMV promoter a...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-010-0942-5
更新日期:2011-03-01 00:00:00
abstract::An in vitro propagation technique based on axillary bud proliferation has been developed for matureSapium sebiferum trees. Nodal segments cultured on Murashige and Skoog (MS) medium supplemented with benzyl adenine (1-10 μM and α-naphthaleneacetic acid (0-0.5 μM showed axillary bud proliferation. Shoots proliferated i...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF01275506
更新日期:1997-06-01 00:00:00
abstract::Seeds are heterogeneous storage reserves with wide array of storage compounds that include various soluble carbohydrates, starch polymer, storage proteins and lipids. These stored reserves comprise 70% of the world's caloric intake in the form of food and animal feed produced through sustainable agriculture, which con...
journal_title:Plant cell reports
pub_type: 社论
doi:10.1007/s00299-017-2135-y
更新日期:2017-05-01 00:00:00
abstract:KEY MESSAGE:The oil palm EgPAL1 gene promoter and its regulatory region were functional as a promoter in the heterologous system of Arabidopsis according to the cis-acting elements present in that region. The promoter was developmentally regulated, vascular tissue specific and responsive to water stress agents. Phenyla...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-017-2228-7
更新日期:2018-02-01 00:00:00
abstract::Scanning electron microscopy has been used to investigate the morphological changes occurring during the development of alfalfa somatic embryos. Embryos were initiated from callus, transferred to suspension culture and matured on solid agar medium. This developmental pattern was compared to that of zygotic embryos dev...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00235082
更新日期:1992-06-01 00:00:00
abstract::Tillering is an important trait of cereal crops that optimizes plant architecture for maximum yield. Teosinte Branched 1 (TB1) is a negative regulator of lateral branching and an inducer of female inflorescence formation in Zea mays (maize). Recent studies indicate that TB1 homologs in Oryza sativa (rice), Sorghum bic...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-011-1139-2
更新日期:2012-01-01 00:00:00
abstract::The effect of various inhibitors on differentiation (shoot morphogenesis) in callus cultures of Brassica, Datura and Nicotiana has been investigated. Hormone medium without any inhibitor (control), resulted in 6% shoot formation. Addition of inhibitors such as actinomycin D, cordycepin, abscisic acid, trigonelline and...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00270062
更新日期:1990-03-01 00:00:00
abstract::Diuraphis noxia (Russian wheat aphid, RWA) is a major pest on wheat in South Africa and most other wheat growing countries. Being a probing-sucking insect, RWAs insert their stylets into the phloem sieve elements and feed on the phloem sap. This feeding causes necrotic lesions in resistant varieties, or decoloration o...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-005-0001-9
更新日期:2006-02-01 00:00:00
abstract::The ample supply of O2 proved to be of great importance for berberine production in cell suspension culture ofThalictrum minus, as the specific O2 consumption rate of berberine-producing cells was twice as high as that of non-producing cells. Furthermore, berberine yield increased with increases in the volumetric O2 t...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00778546
更新日期:1989-04-01 00:00:00