Callus formation from protoplasts of a sugarbeet cell suspension culture.

Abstract:

:Protoplasts of sugarbeet (Beta vulgaris L.) were isolated from cell suspension cultures and cultured in modified PGo medium. Conditions required for the efficient division of the protoplasts were investigated.The optimal combination of phytohormones was found to be 1 mg/l NAA, 0.2 mg/l 2,4-D, 0.5 mg/l zeatin. Protoplast division was also considerably stimulated by the addition of 250 mg/l casein hydrolysate, 200 mg/l yeast extract, and 20% v/v conditioned culture medium to the protoplast culture medium. The highest division rate (up to 35% of the protoplasts) was achieved at a density of 4×10(4)- 1×10(5) protoplasts/ml. From the colonies callus and suspension cultures were readily obtained.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Szabados L,Gaggero C

doi

10.1007/BF00269287

subject

Has Abstract

pub_date

1985-08-01 00:00:00

pages

195-8

issue

4

eissn

0721-7714

issn

1432-203X

journal_volume

4

pub_type

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