Plant regeneration in pigeonpea [Cajanus cajan (L.) Millsp.] by organogenesis.

Abstract:

:A method for regenerating pigeonpea [Cajanus cajan (L.) Millsp.] plants has been developed using distal cotyledonary segments of mature seeds as explants. A large number of shoot buds were induced directly from explants of genotypes T-15-15 and GAUT-82-90 when cultured on six different basal media fortified with 22.2 μM N6-benzylaminopurine, 2.3 μM kinetin, and 271 μM adenine sulfate. The shoot buds developed into shoots when they were subcultured on the same medium but with one-tenth concentrations of cytokinins and adenine sulfate. The shoots elongated by subculturing first two to three times on Murashige and Skoog (MS) basal medium supplemented with 2.22 μM N6-benzylaminopurine and 0.54 μM α-naphthaleneacetic acid or on half-strength MS medium containing 2.89 μM gibberellic acid, and then once on the same medium without growth regulators. Elongated shoots were rooted with 80-85% efficiency on MS medium with 4.92 μM indole-3-butyric acid and the plantlets were transferred for hardening. Plants survival in pots was 70-75%. This method may be useful for improving the crop through genetic manipulations.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Mohan ML,Krishnamurthy KV

doi

10.1007/s002990050469

subject

Has Abstract

pub_date

1998-06-01 00:00:00

pages

705-710

issue

9

eissn

0721-7714

issn

1432-203X

pii

10.1007/s002990050469

journal_volume

17

pub_type

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