The role of the conversion of IBA into IAA on root regeneration in apple: introduction of a test system.

Abstract:

:A test system for root regeneration was developed that consists of stem slices of apple shoots (ca. 0.5 mm thick; fresh weight ca. 1 mg). Roots regenerated synchronously without intervening callus formation and without interference with compounds originating from other parts of the plant. Supply of indolebutyric acid (IBA) or indoleacetic acid (IAA) induced maximally an average of 8 or 4.5 roots per slice, respectively. After uptake of IBA, a high degree of conjugation resulted in a recovery of 2.5% as internal free IBA (ca. 2 times the medium concentration). Due to conversion of absorbed IBA into IAA a fraction of 0.4% was recovered as (physiologically active) free IAA. After incubation on medium with IAA, 0.5% of the absorbed hormone was recovered in the free acid form. No conversion of IAA into IBA was observed. Equimolar contents of internal free IAA after incubation on IBA or IAA resulted for IBA in a higher number of roots than for IAA. This means that IBA may also act via internal free IBA or may synergistically modify the action or endogenous synthesis of IAA.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Van der Krieken WM,Breteler H,Visser MH,Mavridou D

doi

10.1007/BF00237054

subject

Has Abstract

pub_date

1993-02-01 00:00:00

pages

203-6

issue

4

eissn

0721-7714

issn

1432-203X

journal_volume

12

pub_type

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