Abstract:
:A test system for root regeneration was developed that consists of stem slices of apple shoots (ca. 0.5 mm thick; fresh weight ca. 1 mg). Roots regenerated synchronously without intervening callus formation and without interference with compounds originating from other parts of the plant. Supply of indolebutyric acid (IBA) or indoleacetic acid (IAA) induced maximally an average of 8 or 4.5 roots per slice, respectively. After uptake of IBA, a high degree of conjugation resulted in a recovery of 2.5% as internal free IBA (ca. 2 times the medium concentration). Due to conversion of absorbed IBA into IAA a fraction of 0.4% was recovered as (physiologically active) free IAA. After incubation on medium with IAA, 0.5% of the absorbed hormone was recovered in the free acid form. No conversion of IAA into IBA was observed. Equimolar contents of internal free IAA after incubation on IBA or IAA resulted for IBA in a higher number of roots than for IAA. This means that IBA may also act via internal free IBA or may synergistically modify the action or endogenous synthesis of IAA.
journal_name
Plant Cell Repjournal_title
Plant cell reportsauthors
Van der Krieken WM,Breteler H,Visser MH,Mavridou Ddoi
10.1007/BF00237054subject
Has Abstractpub_date
1993-02-01 00:00:00pages
203-6issue
4eissn
0721-7714issn
1432-203Xjournal_volume
12pub_type
杂志文章abstract::Photosynthetically active callus and cell suspension cultures were established from uninfected Lycopersicon peruvianum plants and from uninfected and potato spindle tuber viroid (PSTVd) infected plants of Lycopersicon esculentum cv. Rutgers. Viroid infection was maintained in photoheterotrophic culture on media contai...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232806
更新日期:1993-09-01 00:00:00
abstract::Microcallus (more than 60 cells) formation was obtained from leaf mesophyll protoplasts of 6 species and varieties in the genus Actinidia Lindl. (kiwifruit). The best results were achieved by using liquid over agarose culture for A. arguta var. arguta, liquid and agarose disc type culture for A. arguta var. purpurea, ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00231583
更新日期:1996-09-01 00:00:00
abstract::Separation of stem proteins by sodium dodecyl sulfate polyacrylamide gel electrophoresis permitted classification of 17 celery varieties (Apium graveolens L.) into 7 groups. Several of these groups could be further subdivided into 11 subgroups with gel electrophoresis patterns of 4 isozyme systems. Classification from...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00276666
更新日期:1987-04-01 00:00:00
abstract:KEY MESSAGE:Tetraploid clementine displays mainly tetrasomic inheritance. Genetic structures of 2n SDR and 2 × gametes from DD clementine are complementary and will guides triploids citrus breeding strategies. Triploid breeding is developed worldwide to create new seedless cultivars. Citrus triploid hybrids can be reco...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-016-1972-4
更新日期:2016-08-01 00:00:00
abstract::The Golgi apparatus in plants is organized as a multitude of individual stacks that are motile in the cytoplasm and in close association with the endoplasmic reticulum (ER) (Boevink et al. in Plant J 15:441-447, 1998). These stacks operate as a sorting centre for cargo molecules, providing modification and redirection...
journal_title:Plant cell reports
pub_type: 杂志文章,评审
doi:10.1007/s00299-007-0390-z
更新日期:2007-09-01 00:00:00
abstract::Transgenic papaya (Carica papaya L.) plants were regenerated from embryogenic cultures that were cocultivated with a disarmed C58 strain of Agrobacterium tumefaciens containing one of the following binary cosmid vectors: pGA482GG or pGA482GG/cpPRV-4. The T-DNA region of both binary vectors includes the chimeric genes ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00237128
更新日期:1993-03-01 00:00:00
abstract::Five varieties of durum wheat: Appulo, Ofanto, Latino, Creso, and Castello (Triticum durum Desf.) adapted to the semi-arid mediterranean environment have been tested for their in vitro response. Compact, embryogenic, highly regenerable calli originated from primary callus derived through proliferation of the scutellum...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00193145
更新日期:1991-09-01 00:00:00
abstract::Companion cells are metabolically active and functionally specialized cells that behave as terminals for the transport of materials between phloem and the surrounding tissue. Although previous research has clarified the distinct function of companion cells, it is still largely unknown how plants establish and maintain...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-011-1080-4
更新日期:2011-09-01 00:00:00
abstract::In vitro clonal propagation of plants should generate identical copies of the selected genotype. However, associated stress might result in a breakdown of control mechanisms and consequent instability of the genome. We have used several molecular methods to assess the genetic stability of long-term propagated (24 year...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-007-0413-9
更新日期:2007-11-01 00:00:00
abstract::Plant meiotic prophase I is a complicated process involving the late zygotene and pachytene stages, both crucial for completing synapsis and recombination. Using David Lily (Lilium davidii var. Willmottiae) as our research material, we performed suppression subtractive hybridization to construct EST library of anthers...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-011-1106-y
更新日期:2011-10-01 00:00:00
abstract::While searching for genes expressed in acid lemon but not in acidless lime pulp, we isolated clone Cl111 which showed the following expression phenotypes: (1) while it was expressed in the ovaries in both varieties, its mRNA was detected only in the pulp of the acid fruit, (2) no or very low expression of the gene was...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-011-1073-3
更新日期:2011-09-01 00:00:00
abstract::Autoclaving is a standard procedure for sterilizing nutrient media for plant tissue cultures. Most tissue cultures are grown at pH 5.2 to 5.8 with pH adjustments being made prior to autoclaving. This paper reports that there are significant differences between initial pH levels and pH levels following autoclaving, par...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269825
更新日期:1986-08-01 00:00:00
abstract:KEY MESSAGE:We describe a steroid-inducible BABY BOOM system that improves plant regeneration in Arabidopsis leaf cultures and yields fertile plants. Regeneration of Arabidopsis thaliana plants for extended periods of time in tissue culture may result in sterile plants. We report here a novel approach for A. thaliana r...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-015-1832-7
更新日期:2015-10-01 00:00:00
abstract::Polyclonal antibodies against a part of pea (Pisum sativum L.) LOXG protein have been raised to study the pattern of distribution of related lipoxygenases in pea carpels. The antiserum recognized three lipoxygenase polypeptides in carpels. One of them became undetectable 24 hours after fruit development induction, sug...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232465
更新日期:1996-04-01 00:00:00
abstract::Kidney vetch seedlings were induced to form hairy roots by inoculating their mesocotyls with the wild-type strain 15834 of Agrobacterium rhizogenes or with the A. tumefaciens strain C58C1 containing a binary vector system (the pRiA4b as a helper and the vector pCB1346 bearing a pTiC58-derived isopentenyl transferase g...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00233367
更新日期:1992-07-01 00:00:00
abstract::Plants have developed diverse molecular mechanisms to resist viruses. RNA silencing plays a dominant role in antiviral defense. Recent studies have correlated plant antiviral silencing to epigenetic modification in genomic DNA and protein by remodeling the expression levels of coding genes. The plant host methylation ...
journal_title:Plant cell reports
pub_type: 杂志文章,评审
doi:10.1007/s00299-019-02414-0
更新日期:2019-09-01 00:00:00
abstract::Conditions necessary for the isolation and culture of protoplasts from suspension cultures of sugar, fodder and garden beets were investigated. Good yields of protoplasts were obtained by treating cells with a mixture of cellulase, Macerozyme and Driselase enzymes. Nutritional requirements of beet protoplasts were fou...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00269896
更新日期:1985-12-01 00:00:00
abstract::A transformed root clone of Datura candida×D. aurea was established following infection with Agrobacterium rhizogenes strain A4. This clone was examined for its growth and hyoscyamine and scopolamine content under various culture conditions. Among the three basal culture media tested, half-strength Gamborg's B5 medium...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990050415
更新日期:1998-03-01 00:00:00
abstract:KEY MESSAGE:We developed a method for detection of mutants in a large number of plants, and found this method to be applicable to detection of a mutant allele at a concentration of 1/1000. Many techniques for SNP analysis have been developed, but most of these techniques are not so sensitive to be used for detection of...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-018-2274-9
更新日期:2018-06-01 00:00:00
abstract::An efficient micropropagation system for Saussurea involucrata Kar. et Kir., an endangered Chinese medicinal plant, has been developed. Shoot organogenesis occurred from S. involucrata leaf explants inoculated on medium with appropriate supplements of plant growth regulators. 66.0% of shoot regeneration frequency and ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-006-0230-6
更新日期:2007-03-01 00:00:00
abstract::Programmed cell death (PCD) is a genetically encoded, active process that results in the death of individual cells, tissues, or whole organs, which plays an important role in the life cycles of plants and animals. Previous studies show that methyl salicylate (MeSA) is a defense signal molecular associated with systemi...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-011-1031-0
更新日期:2011-07-01 00:00:00
abstract::Novel and efficient protocols for plant regeneration and genetic transformation from longitudinally-halved cotyledons ofin vitro raised seedlings in eggplant (Solanum melongena L.) are described. After co-cultivation withAgrobacterium vectors harboring neomycin phosphotransferase (nptll) as selectable marker, transgen...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF01690259
更新日期:1995-01-01 00:00:00
abstract::The interconversion between 5 to 8-membered cycloalkanones and the corresponding cycloalkanols by a cell suspension culture of Nicotiana tabacum was studied. It was found that the interconversion is at equilibrium and the balance in the equilibrium depends on the number of carbon atoms in the carbocyclic ring of the s...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00270167
更新日期:1983-04-01 00:00:00
abstract::Mimosa tenuiflora (Willd.) Poiret (Leguminosae) was micropropagated throughin vitro culture of axillary buds on Murashige and Skoog (MS) medium. Shoot formation was achieved when the media were supplemented with 0.1 mg.L(-1) IAA + 3 mg.L(-1) KN.In vitro rooting of regenerated shoots was achieved when 0.1 mg.L(-1) KN w...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF01275455
更新日期:1996-11-01 00:00:00
abstract::High-frequency bud break and multiple shoots were induced in apical shoot buds and nodal explants ofMorus cathayana, M. lhou andM. serrata on Murashige and Skoog (MS) medium containing 0.5-1.0 mg/l 6-benzylaminopurine (BAP). Addition of gibberellic acid (0.4 mg/l) along with BAP induced faster bud break both in apical...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF01092774
更新日期:1997-04-01 00:00:00
abstract::The conserved domains of reverse transcriptase (RT) genes of Ty1-copia and Ty3-gypsy groups of long terminal repeat (LTR) retrotransposons were amplified from mungbean (Vigna radiata) genome using degenerate primers, cloned and sequenced. Among these 34% and 65% of respective clones of copia and gypsy RT sequences pos...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-005-0008-2
更新日期:2006-03-01 00:00:00
abstract::KEY MESSAGE : Rooting of Artemisia annua increases trichome size on leaves and helps drive the final steps of the biosynthesis of the sesquiterpene antimalarial drug, artemisinin. ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-012-1355-4
更新日期:2013-02-01 00:00:00
abstract::High yields of protoplasts were obtained from leaves of aseptically grown plants and calli originated from different explants, in several cultivars of Cajanus cajan L. The protoplasts divided to form cell clusters in modified KM 8p medium and developed to protocolonies after dilution with liquid Caboche's medium withi...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/BF00232691
更新日期:1992-08-01 00:00:00
abstract::The article 'Instability of endosperm development in amphiploids and their parental species in the genus Avena L.', written by Paulina Tomaszewska and Romuald Kosina, was originally published electronically on the publisher's internet portal on 22 May with an incorrect copyright line. As the article was due to be publ...
journal_title:Plant cell reports
pub_type: 杂志文章,已发布勘误
doi:10.1007/s00299-018-2337-y
更新日期:2019-01-01 00:00:00
abstract::MicroRNAs (miRNAs) are small non-coding RNAs that play vital roles in plant abiotic stress responses via cleavage or translational inhibition of their target mRNAs. Populus euphratica is a typical stress-resistant sessile organism that grows in desert areas. Here, we identified sequences of 12 miRNA precursors from 11...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-011-1096-9
更新日期:2011-10-01 00:00:00