Photosynthetically active suspension cultures of potato spindle tuber viroid infected tomato cells as tools for studying viroid - host cell interaction.

Abstract:

:Photosynthetically active callus and cell suspension cultures were established from uninfected Lycopersicon peruvianum plants and from uninfected and potato spindle tuber viroid (PSTVd) infected plants of Lycopersicon esculentum cv. Rutgers. Viroid infection was maintained in photoheterotrophic culture on media containing 3% sucrose, but during continuous photo-mixotrophic culture in low sucrose media (1% sucrose), the level of PSTVd accumulation decreased. Photoautotrophic cell suspensions could be established with uninfected, but not with viroid infected tomato cells. As compared to uninfected cells, PSTVd infected cells grew slowly, were morphologically different in size and shape, and formed tight cell aggregates. Electronmicroscopy showed that starch accumulation in chloroplasts, deformation of the chloroplast envelope and irregular plasmalemmasomes at the cell membrane were associated with PSTVd infection.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Stöcker S,Guitton MC,Barth A,Mühlbach HP

doi

10.1007/BF00232806

subject

Has Abstract

pub_date

1993-09-01 00:00:00

pages

597-602

issue

11

eissn

0721-7714

issn

1432-203X

journal_volume

12

pub_type

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