Stability of transgene integration and expression in subsequent generations of doubled haploid oilseed rape transformed with chitinase and beta-1,3-glucanase genes in a double-gene construct.

abstract：:In this study, we report the obtaining of carrot plants expressing human interferon alpha-2b via Agrobacterium-mediated transformation using two vector constructs containing the sequence coding for interferon gene fused with Nicotiana plumbagenifolia calreticulin apoplast targeting signal driven by 35S CaMV promoter a...

journal_title：Plant cell reports

authors： Luchakivskaya Y,Kishchenko O,Gerasymenko I,Olevinskaya Z,Simonenko Y,Spivak M,Kuchuk M

更新日期：2011-03-01 00:00:00

abstract：:The in vivo nitrate reductase activity in 8 day old dark-grown sugarcane callus was over three fold that of the light-grown callus. NADH (0.3 mM) in the reaction system, increased the in vivo nitrate reductase activity by more than two fold both in the dark- and the light-grown callus tissues. The NADH dependence of n...

journal_title：Plant cell reports

authors： Khan BM,Dwivedi UN,Rawal SK,Mascarenhas AF

更新日期：1984-08-01 00:00:00

abstract：:Sugarcane cell suspensions were initiated from leaf callus and sub-cultured every 7 to 10 days by alternate transfer to MS based medium with 3.0 and 1.0 mg 1(-1)2,4-D. Suspensions older than 3 months gave the most reproducible yields of protoplasts. Isolated protoplasts required 50 mM Ca(2+) in the washing solution an...

journal_title：Plant cell reports

authors： Chen WH,Davey MR,Power JB,Cocking EC

更新日期：1988-08-01 00:00:00

abstract：:To develop an alternative genetic transformation system that is not dependent on an antibiotic selection strategy, the phosphomannose isomerase gene (pmi) system was evaluated for producing transgenic plants of chickpea (Cicer arietinum L.). A shoot morphogenesis protocol based on the thidiazuron (TDZ)-induced shoot m...

journal_title：Plant cell reports

authors： Patil G,Deokar A,Jain PK,Thengane RJ,Srinivasan R

更新日期：2009-11-01 00:00:00

abstract：KEY MESSAGE:Sugarcane lines transformed with an alanine aminotransferase gene demonstrated an improved nitrogen use efficiency compared with untransformed controls in a pot trial under low nitrogen conditions. ...

journal_title：Plant cell reports

authors： Snyman SJ,Hajari E,Watt MP,Lu Y,Kridl JC

更新日期：2015-05-01 00:00:00

abstract：:Exposure of Arabidopsis thaliana L. to an acclimation temperature (+4°C) results in a rapid increase of frost tolerance from -3°C to -7°C. This increase could be correlated to changes in soluble protein pattern. Analysis of in vitro translation products from isolated mRNA suggests that induction acts at the transcript...

journal_title：Plant cell reports

authors： Kurkela S,Franck M,Heino P,Lång V,Palva ET

更新日期：1988-12-01 00:00:00

abstract：KEY MESSAGE:FLA, the homolog of ubiquitin-specific protease does not have deubiquitination activity, but it is essential for flower and chloroplast development in rice. Ubiquitin-specific proteases (UBPs) are widely distributed and highly conserved proteins and are also members of the most important family of deubiquit...

journal_title：Plant cell reports

authors： Ma X,Zhang J,Han B,Tang J,Cui D,Han L

更新日期：2019-03-01 00:00:00

abstract：:Medicago sativa lines with a high incidence of regeneration were established as suspension cultures and used to select for NaCl tolerant lines. Attempts were then made to regenerate plants from these lines. Regeneration was severely depressed in NaCl tolerant calli and the only plants that were successfully regenerate...

journal_title：Plant cell reports

authors： Smith MK,McComb JA

更新日期：1983-06-01 00:00:00

abstract：:Glyoxalase-I activity in growingDatura callus showed 184% increase with the age of the culture. Spermidine increased the enzyme activity together with DNA and protein synthesis. With the addition of mitotic inhibitors, vinblastine and methylglyoxal in the growth medium, the enzyme activity was inhibited by 92 percent ...

journal_title：Plant cell reports

authors： Ramaswamy O,Pal S,Guha-Mukherjee S,Sopory SK

更新日期：1984-06-01 00:00:00

abstract：:Brassica juncea L. is a Zn/Cd accumulator. To determine the physiological basis of its metal accumulation phenotype, the functional properties and role of the metal efflux transporter BjCET2 were investigated using transgenic technology. Heterologous expression of BjCET2 in the double mutant yeast strain Deltazrc1Delt...

journal_title：Plant cell reports

authors： Xu J,Chai T,Zhang Y,Lang M,Han L

更新日期：2009-08-01 00:00:00

abstract：:Plum hypocotyl slices were transformed with the coat protein (CP) gene of plum pox virus (PPV-CP) following cocultivation with Agrobacterium tumefaciens containing the plasmid pGA482GG/PPVCP-33. This binary vector carries the PPV-CP gene construct, as well as the chimeric neomycin phosphotransferase and β-glucuronidas...

journal_title：Plant cell reports

authors： Scorza R,Ravelonandro M,Callahan AM,Cordts JM,Fuchs M,Dunez J,Gonsalves D

更新日期：1994-11-01 00:00:00

abstract：KEY MESSAGE:A novel Zoysia japonica salt-induced glycine-rich RNA-binding protein gene was cloned in this study and its overexpression caused salt sensitivity in transgenic Arabidopsis. Glycine-rich RNA-binding proteins (GRPs) play crucial roles in diverse plant developmental processes. However, the mechanisms and func...

journal_title：Plant cell reports

authors： Teng K,Tan P,Xiao G,Han L,Chang Z,Chao Y

更新日期：2017-01-01 00:00:00

abstract：:Phenylalanine ammonia-lyase [PAL, EC 4.3.1.24 (formerly EC 4.3.1.5)], functions in the plant phenylpropanoid biosynthetic pathway to deaminate the amino acid L-phenylalanine forming trans-cinnamic acid and ammonia. The human inherited metabolic disorder phenylketonuria (PKU) is characterized by an inability of individ...

journal_title：Plant cell reports

authors： McInnis S,Clemens S,Kermode AR

更新日期：2009-03-01 00:00:00

abstract：:Three O-methyltransferases which catalyze S-adenosyl-L-methionine (SAM)-dependent O-methylation of licodione (LMT), flavone/flavonol (FMT), and caffeic acid (CMT) were separated from the callus culture of Glycyrrhiza echinata, and characteristic differences between their pH optima and Mg(2+) requirement for activity w...

journal_title：Plant cell reports

authors： Ayabe S,Udagawa A,Iida K,Yoshikawa T,Furuya T

更新日期：1987-02-01 00:00:00

abstract：:Cell suspension cultures of cowpea (Vigna sp.) were able to produce extracellular peroxidase. Different growth regulator concentrations induced different peroxidase activity in callus. The crude extracellular medium after four weeks of culture showed higher (6 times) specific peroxidase activity and higher thermo stab...

journal_title：Plant cell reports

authors： Moreno OA,Vazquez-Duhalt R,Nolasco H

更新日期：1990-07-01 00:00:00

abstract：:Rosmarinic acid (RA) is a natural antioxidant produced by cell suspension cultures of sage (Salvia officinalis L.). The growth and production of RA by these cells can be modified by the type of culture medium. Production can be increased 10-fold to attain 6.4 g.1(-1) under optimal conditions. Investigation of kinetics...

journal_title：Plant cell reports

authors： Hippolyte I,Marin B,Baccou JC,Jonard R

更新日期：1992-04-01 00:00:00

abstract：:An efficient micropropagation system for Saussurea involucrata Kar. et Kir., an endangered Chinese medicinal plant, has been developed. Shoot organogenesis occurred from S. involucrata leaf explants inoculated on medium with appropriate supplements of plant growth regulators. 66.0% of shoot regeneration frequency and ...

journal_title：Plant cell reports

authors： Guo B,Gao M,Liu CZ

更新日期：2007-03-01 00:00:00

abstract：KEY MESSAGE:MicroRNAs have higher expression stability than protein-coding genes in B. napus seeds and are therefore good reference genes for miRNA and mRNA RT-qPCR analysis. Reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) has become the "gold standard" to gain insight into function of ...

journal_title：Plant cell reports

authors： Machado RD,Christoff AP,Loss-Morais G,Margis-Pinheiro M,Margis R,Körbes AP

更新日期：2015-07-01 00:00:00

abstract：:Yucca valida is an important potential source of steroidal saponins closely related to Yucca schidigera, the species that is commercially exploited from the wild as a source of steroidal extracts. Neither of the species has been domesticated mainly because of their slow growth and long life span before harvesting. Her...

journal_title：Plant cell reports

authors： Arce-Montoya M,Rodríguez-Alvarez M,Hernández-González JA,Robert ML

更新日期：2006-08-01 00:00:00

abstract：:A cDNA clone encoding a putative RTE1-like protein (Rh-RTH1) was obtained from total RNA isolated from senescing rose (Rosa hybrida cv. Tineke) petals using RT-PCR and RACE techniques. The cDNA (1,061 bp) contained an open reading frame of 684 bp corresponding to 227 amino acids. The amino acid sequence had 60.0, 49.6...

journal_title：Plant cell reports

authors： Yu Y,Wang J,Wang H,Zhang Z,Liu J

更新日期：2010-08-01 00:00:00

abstract：:To improve turfgrasses using genetic engineering, we have developed a transformation system in turf-type tall fescue, one of the most important turfgrass species. Embryogenic cell cultures were established after callus induction from embryos of mature seed. The agarose-bead method with nurse cells was used to culture ...

journal_title：Plant cell reports

authors： Ha SB,Wu FS,Thorne TK

更新日期：1992-11-01 00:00:00

abstract：:Protoplasts were isolated from embryogenic callus ofAbies alba L. which originated from immature seeds. The protoplasts were immobilized in alginate beads in order to follow the development of single protoplasts.Surrounding culture medium was modified from Kao and Michayluk (1975). After cell wall regeneration subsequ...

journal_title：Plant cell reports

authors： Lang H,Kohlenbach HW

更新日期：1989-02-01 00:00:00

abstract：:Leaves excised from shoot cultures of Prunus avium cvs. F12/1 and Charger and genotype 1908, and from five genotypes of P. serotina and two hybrids of P. avium×P. sargentii developed shoots on Woody Plant medium (WPM) supplemented with either benzyladenine (BA) or thidiazuron (TDZ). Regeneration in both P. avium 1908 ...

journal_title：Plant cell reports

authors： Hammatt N,Grant NJ

更新日期：1998-04-01 00:00:00

abstract：:Hairy root culture of Ginseng (Panax ginseng) was established after roots were induced on callus following infection with Agrobacterium rhizogenes. The transformed cultures of ginseng could be subcultured as an axenic root culture in the absence of phytohormones, and grew with extensive lateral branches more rapidly t...

journal_title：Plant cell reports

authors： Yoshikawa T,Furuya T

更新日期：1987-12-01 00:00:00

abstract：: Cells of a suspension culture of Doritaenopsis cv. New Toyohashi were placed in a mixture of 2 M glycerol and 0.4 M sucrose for 15 min at room temperature and then dehydrated with a vitrification solution (PVS2) for 1-3 h on ice and plunged into liquid nitrogen. The highest viability (64% by 2,3,5-triphenyltetrazoliu...

journal_title：Plant cell reports

authors： Tsukazaki H,Mii M,Tokuhara K,Ishikawa K

更新日期：2000-12-01 00:00:00

abstract：:DNA fingerprints generated by the Jeffreys' probes, 33.6 and 33.15, indicated the presence of minisatellite-like sequences in the red clover genome. The fingerprints generated by probe 33.6 gave less background and fewer but better defined bands than those obtained with probe 33.15. Assay of a regenerative somaclonal ...

journal_title：Plant cell reports

authors： Nelke M,Nowak J,Wright JM,McLean NL

更新日期：1993-12-01 00:00:00

abstract：:We developed an efficient plant regeneration system from protoplasts for poplar (Populus alba L.). Protoplasts were isolated from 4-day-old suspension cultures derived from seed-induced calli with a yield of 6.96× 106 cells/g fresh weight cells and then cultured at a concentration of 2.5×105 cells/ml in NH4NO3-free Mu...

journal_title：Plant cell reports

authors： Qiao J,Kuroda H,Hayashi T,Sakai F

更新日期：1998-01-01 00:00:00

abstract：:Rapid clonal propagation in three stolon bearing species of Nephrolepis was achieved by tissue culture methods. Explants from young stolons were grown on B5 (Gamborg et al. 1968) medium supplemented with indolebutyric acid where they produced several leaf primordia. These leaf primordia were isolated and grown into en...

journal_title：Plant cell reports

authors： Padhya MA,Mehta AR

更新日期：1982-12-01 00:00:00

abstract：:Green fluorescent protein (GFP) has been used widely as a powerful bioluminescent reporter, but its visualization by existing methods in tissues or whole plants and its utilization for high-throughput screening remains challenging in many species. Here, we report a fluorescence image analyzer-based method for GFP dete...

journal_title：Plant cell reports

authors： Hily JM,Liu Z

更新日期：2009-03-01 00:00:00

abstract：:Rhodamine-6G(R6G), a lipophilic dye which degrades mammalian mitochondria, was shown to arrest the division of Nicotiana protoplasts. When albino recipient-protoplasts were treated with R6G and fused with X-irradiated (green) donor- protoplasts, only green cybrid plants were obtained. The mtDNA of the cybrids was anal...

journal_title：Plant cell reports

authors： Aviv D,Chen R,Galun E

更新日期：1986-06-01 00:00:00