5'-Anchored simple-sequence repeat primers are useful for analysing potato somatic hybrids.

Abstract:

:Somatic hybrids between Solanum tuberosum cv 'Brodick' and S. sanctae-rosae were selected for having elevated chromosome numbers, intermediate morphology and potato cyst nematode resistance (derived from S. sanctae-rosae). DNA was extracted from this material and subjected to inter-SSR PCR directed by oligonucleotide primers comprising 5'-anchored di- and tri-nucleotide repeat motifs. PCR products were separated by polyacrylamide gel electrophoresis and visualised by silver staining. An average of 30.31 bands per primer were generated in each parent and, on average, 8.19 of these bands were polymorphic. S. sanctae-rosae-specific bands were observed in the hybrid material as was evidence of deletions, substitutions and rearrangements. The relative merits of this technique for the analysis of somatic hybrid material are discussed.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Matthews D,McNicoll J,Harding K,Millam S

doi

10.1007/s002990050735

subject

Has Abstract

pub_date

1999-12-01 00:00:00

pages

210-212

issue

2

eissn

0721-7714

issn

1432-203X

pii

10.1007/s002990050735

journal_volume

19

pub_type

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