Comparison of genetic transformation in Morus alba L. via different regeneration systems.

Abstract:

:Three different regeneration systems, viz. direct regeneration of adventitious shoot buds from explant, regeneration through callus cultures and somatic embryos were compared to see their effect on transfer of neomycin phosphotransferase (nptII) and beta-glucuronidase (GUS) reporter gene (gus) to Morus alba clone M5, through Agrobacterium tumefaciens mediated transformation. Pre-conditioning and co-cultivation durations had a marked effect on transformation frequency. The highest transformation frequency of 18.6% was obtained using direct induction of adventitious shoot buds. Expression and presence of transgene were assayed histochemically and through polymerase chain reaction. Southern analysis of GUS and PCR positive transformants confirmed stable integration of transgenes with two to four copy numbers. The selected transformants showed normal phenotype under in vitro and field conditions.

journal_name

Plant Cell Rep

journal_title

Plant cell reports

authors

Agarwal S,Kanwar K

doi

10.1007/s00299-006-0217-3

subject

Has Abstract

pub_date

2007-02-01 00:00:00

pages

177-85

issue

2

eissn

0721-7714

issn

1432-203X

journal_volume

26

pub_type

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