MicroRNA-138 Inhibits Periodontal Progenitor Differentiation under Inflammatory Conditions.


:Inflammatory conditions as they occur during periodontal disease often result in decreased alveolar bone levels and a loss of connective tissue homeostasis. Here we have focused on the effect of microRNA-138 (miR-138) as a potential regulator of periodontal stem cells as they affect homeostasis during inflammatory conditions. Our data indicate that miR-138 was significantly upregulated in our periodontal disease animal model. Interaction of miR-138 with a predicted targeting site on the osteocalcin (OC) promoter resulted in a 3.7-fold reduction of luciferase activity in promoter assays compared with controls; and miR-138 overexpression in periodontal progenitors significantly inhibited OC (3.4-fold), Runx2 (2.8-fold), and collagen I (2.6-fold). Moreover, treatment with inflammatory modulators such as interleukin (IL)-6 and lipopolysaccharide (LPS) resulted in a significant 2.2-fold (IL-6) or 1.9-fold (LPS) increase in miR-138 expression, while OC and Runx2 expression was significantly decreased as a result of treatment with each inflammatory mediator. Further defining the role of miR-138 in the OC-mediated control of mineralization, we demonstrated that the LPS-induced downregulation of OC expression was partially reversed after miR-138 knockdown. LPS, miR-138 mimic, and OC small interfering RNA inhibited osteoblast differentiation marker alkaline phosphatase activity, while miR-138 inhibitor and OC protein addition enhanced alkaline phosphatase activity. Supporting the role of OC as an essential modulator of osteoblast differentiation, knockdown of miR-138 or addition of OC protein partially rescued alkaline phosphatase activity in periodontal ligament (PDL) cells subjected to LPS treatment. Our data establish miR-138 inhibitor as a potential therapeutic agent for the prevention of the bone loss associated with advanced periodontal disease.


J Dent Res


Zhou X,Luan X,Chen Z,Francis M,Gopinathan G,Li W,Lu X,Li S,Wu C,Diekwisch TG




Has Abstract


2016-02-01 00:00:00














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    journal_title:Journal of dental research

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    authors: Urata K,Shinoda M,Honda K,Lee J,Maruno M,Ito R,Gionhaku N,Iwata K

    更新日期:2015-03-01 00:00:00

  • Novel PAX9 mutations cause non-syndromic tooth agenesis.

    abstract::PAX9 is a transcription factor expressed in the tooth mesenchyme during tooth morphogenesis. In Pax9-null mice, tooth development is arrested at the bud stage. In humans, heterozygous mutations in PAX9 have been associated with non-syndromic tooth agenesis, predominantly in the molars. Here, we report 2 novel mutation...

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    authors: Mitsui SN,Yasue A,Masuda K,Watanabe K,Horiuchi S,Imoto I,Tanaka E

    更新日期:2014-03-01 00:00:00

  • Sleep apnea symptoms and risk of temporomandibular disorder: OPPERA cohort.

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    journal_title:Journal of dental research

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    authors: Sanders AE,Essick GK,Fillingim R,Knott C,Ohrbach R,Greenspan JD,Diatchenko L,Maixner W,Dubner R,Bair E,Miller VE,Slade GD

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  • Microbial aspects of preventive regimes in patients with overdentures.

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    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Keltjens HM,Schaeken MJ,Van der Hoeven JS

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  • SHED differentiate into functional odontoblasts and endothelium.

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    journal_title:Journal of dental research

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    journal_title:Journal of dental research

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    authors: Sakagami J,Ono T,Hasegawa Y,Hori K,Zhang M,Maeda Y

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  • Anatomic and directional variation in the mechanical properties of the mandibular condyle in pigs.

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    authors: Teng S,Herring SW

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  • The resistance and adaptation of selected oral bacteria to mercury and its impact on their growth.

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    journal_title:Journal of dental research

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    authors: Lyttle HA,Bowden GH

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    journal_title:Journal of dental research

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    authors: Peters OA,Laib A,Rüegsegger P,Barbakow F

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  • Comparison of in vitro and in vivo wear of composites.

    abstract::In vitro wear data were determined for nine experimental composites and compared with two-year clinical data. The in vitro tests included single-pass wear and two-body abrasion. The in vivo data were obtained between 1978 and 1980 for 54 class 2 posterior composite restorations in 25 patients. Correlations between the...

    journal_title:Journal of dental research

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    authors: Momoi Y,Iwase H,Nakano Y,Kohno A,Asanuma A,Yanagisawa K

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    pub_type: 杂志文章


    authors: Stinson MW,Levine MJ,Cavese JM,Prakobphol A,Murray PA,Tabak LA,Reddy MS

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    更新日期:2014-03-01 00:00:00