MicroRNA-138 Inhibits Periodontal Progenitor Differentiation under Inflammatory Conditions.


:Inflammatory conditions as they occur during periodontal disease often result in decreased alveolar bone levels and a loss of connective tissue homeostasis. Here we have focused on the effect of microRNA-138 (miR-138) as a potential regulator of periodontal stem cells as they affect homeostasis during inflammatory conditions. Our data indicate that miR-138 was significantly upregulated in our periodontal disease animal model. Interaction of miR-138 with a predicted targeting site on the osteocalcin (OC) promoter resulted in a 3.7-fold reduction of luciferase activity in promoter assays compared with controls; and miR-138 overexpression in periodontal progenitors significantly inhibited OC (3.4-fold), Runx2 (2.8-fold), and collagen I (2.6-fold). Moreover, treatment with inflammatory modulators such as interleukin (IL)-6 and lipopolysaccharide (LPS) resulted in a significant 2.2-fold (IL-6) or 1.9-fold (LPS) increase in miR-138 expression, while OC and Runx2 expression was significantly decreased as a result of treatment with each inflammatory mediator. Further defining the role of miR-138 in the OC-mediated control of mineralization, we demonstrated that the LPS-induced downregulation of OC expression was partially reversed after miR-138 knockdown. LPS, miR-138 mimic, and OC small interfering RNA inhibited osteoblast differentiation marker alkaline phosphatase activity, while miR-138 inhibitor and OC protein addition enhanced alkaline phosphatase activity. Supporting the role of OC as an essential modulator of osteoblast differentiation, knockdown of miR-138 or addition of OC protein partially rescued alkaline phosphatase activity in periodontal ligament (PDL) cells subjected to LPS treatment. Our data establish miR-138 inhibitor as a potential therapeutic agent for the prevention of the bone loss associated with advanced periodontal disease.


J Dent Res


Zhou X,Luan X,Chen Z,Francis M,Gopinathan G,Li W,Lu X,Li S,Wu C,Diekwisch TG




Has Abstract


2016-02-01 00:00:00














  • Fluoride deposition in human enamel in vivo from professionally applied fluoride prophylaxis paste.

    abstract::Deciduous teeth were collected from children who received professional prophylaxis with an acidulated phosphate-fluoride (APF) or placebo paste and were analyzed to determine fluoride uptake. Enamel fluoride level was increased by the APF paste approximately 300 ppm at a depth of 2.5 micrometers during the first month...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Mellberg JR,Nicholson CR,Ripa LW,Barenie J

    更新日期:1976-11-01 00:00:00

  • The biocompatibility of dental implant materials measured in an animal model.

    abstract::The biocompatibility of two implant materials, vitallium and poly-methyl methacrylate (PMMA), was evaluated in an animal model in which bone formation and resorption are independent of each other. Neither material accelerated resorption, but formation was inhibited 12% by vitallium and 38% by PMMA. When three PMMA pre...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Garcia DA,Sullivan TM,O'Neill DM

    更新日期:1981-01-01 00:00:00

  • The influence of polyacid molecular weight on some properties of glass-ionomer cements.

    abstract::The influence of the molecular weight of the poly(acrylic acid) component on some properties of glass-ionomer cement has been investigated. The results can be explained by treatment of glass-ionomer cements as thermoplastic composites. Many of the concepts of polymer science can be applied successfully in a qualitativ...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Wilson AD,Hill RG,Warrens CP,Lewis BG

    更新日期:1989-02-01 00:00:00

  • Distribution and excretion of TEGDMA in guinea pigs and mice.

    abstract::The monomer triethyleneglycoldimethacrylate (TEGDMA) is used as a diluent in many resin-based dental materials. It was previously shown in vitro that TEGDMA was released into the adjacent biophase from such materials during the first days after placement. In this study, the uptake, distribution, and excretion of 14C-T...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Reichl FX,Durner J,Hickel R,Kunzelmann KH,Jewett A,Wang MY,Spahl W,Kreppel H,Moes GW,Kehe K,Walther U,Forth W,Hume WR

    更新日期:2001-05-01 00:00:00

  • A comparison of S. mutans prevalence in relation to caries experience in Norwegian and immigrant Vietnamese children.

    abstract::Vietnamese immigrant and Norwegian children, matched according to caries experience, age, and sex, were compared with respect to S. mutans in dental plaque and in saliva. Regardless of ethnic group, S. mutans prevalence was highest in plaque over caries lesions and from fissures, and lowest in plaque from smooth surfa...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Scheie AA,Selikowitz HS,Arneberg P

    更新日期:1984-12-01 00:00:00

  • Stain removal from a silicone maxillofacial elastomer.

    abstract::In this study, environmental stains were removed from maxillofacial elastomers by solvent extraction. Silastic 44210, an RTV silicone with proven color and physical property stability, was stained with lipstick, disclosing solution, and methylene blue. These stains were then removed by solvent extraction with each of ...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Yu R,Koran A,Raptis CN,Craig RG

    更新日期:1981-10-01 00:00:00

  • Trigeminal nociceptors express TLR-4 and CD14: a mechanism for pain due to infection.

    abstract::Although certain bacterial species appear to be risk factors for pain due to odontogenic infections, comparatively little is known about the potential mechanisms mediating this effect. In this study, we tested the hypothesis that trigeminal nociceptive neurons express the TLR4 or CD14 receptors, thus enabling sensory ...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Wadachi R,Hargreaves KM

    更新日期:2006-01-01 00:00:00

  • The hydration of dental cements.

    abstract::A study was made of the hydration of dental cements, water being classified as "non-evaporable" and "evaporable". The ratio of these two types of water was found to vary greatly among different cement types, being lesser in zinc oxide and ionic polymer cements and greater in ion-leachable glass and phosphoric acid cem...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Wilson AD,Paddon JM,Crisp S

    更新日期:1979-03-01 00:00:00

  • Estimation of mesiodistal width of permanent canines and premolars in early mixed dentition.

    abstract::The accuracy for predicting mesiodistal widths of unerupted permanent canines and premolars from x-rays and their estimations based on the already erupted permanent teeth were tested in a group of Israeli children. The observed posteruptive widths related more closely to predicted values obtained from x-ray measuremen...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Zilberman Y,Koyoumdjisky-Kaye E,Vardimon A

    更新日期:1977-08-01 00:00:00

  • Saliva/pathogen biomarker signatures and periodontal disease progression.

    abstract::The purpose of this study was to determine the role of saliva-derived biomarkers and periodontal pathogens during periodontal disease progression (PDP). One hundred human participants were recruited into a 12-month investigation. They were seen bi-monthly for saliva and clinical measures and bi-annually for subtractio...

    journal_title:Journal of dental research

    pub_type: 临床试验,杂志文章


    authors: Kinney JS,Morelli T,Braun T,Ramseier CA,Herr AE,Sugai JV,Shelburne CE,Rayburn LA,Singh AK,Giannobile WV

    更新日期:2011-06-01 00:00:00

  • Particle size distribution of food boluses after mastication of six natural foods.

    abstract::There is a large variability between and among individuals in the physiology of mastication, but it is not known whether this produces a similar variability in the particle sizes of food boluses at the end of the chewing process. Food boluses obtained just before swallowing were analyzed in ten subjects (aged 36.7 +/-...

    journal_title:Journal of dental research

    pub_type: 临床试验,杂志文章


    authors: Peyron MA,Mishellany A,Woda A

    更新日期:2004-07-01 00:00:00

  • Immune dysfunction and dental caries: a preliminary report.

    abstract::This study was undertaken in order to correlate the immune status of persons with various types of immune dysfunctions with the incidence of dental caries. Preliminary data on caries experience and the microbial composition of plaque in these individuals are presented. The findings suggest that persons with immunoglob...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Cole MF,Arnold RR,Rhodes MJ,McGhee JR

    更新日期:1977-03-01 00:00:00

  • Enhancing oral health via sense of coherence: a cluster-randomized trial.

    abstract::Sense of coherence (SOC) has been related to oral health behaviors and oral-health-related quality of life (OHRQoL) in observational studies. This cluster-randomized trial aimed to test the effect of an intervention to enhance SOC on OHRQoL in children. Twelve primary schools were randomly allocated to intervention an...

    journal_title:Journal of dental research

    pub_type: 杂志文章,随机对照试验


    authors: Nammontri O,Robinson PG,Baker SR

    更新日期:2013-01-01 00:00:00

  • A community-based RCT for oral cancer screening with toluidine blue.

    abstract::Early detection of oral premalignant lesions (OPMLs) by visual inspection with toluidine blue has not been addressed. We conducted a community-based randomized controlled trial to assess whether using toluidine blue as an adjunctive tool for visual screening had a higher detection rate of OPMLs and could further reduc...

    journal_title:Journal of dental research

    pub_type: 杂志文章,随机对照试验


    authors: Su WW,Yen AM,Chiu SY,Chen TH

    更新日期:2010-09-01 00:00:00

  • Fluoridated Water Modifies the Effect of Breastfeeding on Dental Caries.

    abstract::Breastfeeding is important for health and development. Yet, the interaction between breastfeeding duration and usage of fluoridated water on caries experience has not been investigated. This study examined exposure to fluoridation as an effect modifier of the association between breastfeeding duration and caries. The ...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Ha DH,Spencer AJ,Peres KG,Rugg-Gunn AJ,Scott JA,Do LG

    更新日期:2019-07-01 00:00:00

  • Clinical effectiveness of orthodontic miniscrew implants: a meta-analysis.

    abstract::The aim of this meta-analysis was to examine the clinical effectiveness of miniscrew implants (MI) used for anchorage reinforcement compared with that of conventional orthodontic means, as well as to assess the success rates of MIs and the possible risk factors affecting their clinical effectiveness. Literature search...

    journal_title:Journal of dental research

    pub_type: 杂志文章,meta分析,评审


    authors: Papadopoulos MA,Papageorgiou SN,Zogakis IP

    更新日期:2011-08-01 00:00:00

  • A major gene effect controls resistance to caries.

    abstract::Despite recent advances revealing genetic factors influencing caries susceptibility, questions regarding the model of inheritance involved are yet to be addressed. We conducted a Complex Segregation Analysis on decayed teeth in a sample of homogenous, isolated families recruited from the Brazilian Amazon. A dominant, ...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Werneck RI,Lázaro FP,Cobat A,Grant AV,Xavier MB,Abel L,Alcaïs A,Trevilatto PC,Mira MT

    更新日期:2011-06-01 00:00:00

  • Full- vs. partial-mouth disinfection in the treatment of periodontal infections: short-term clinical and microbiological observations.

    abstract::In a standard periodontal treatment strategy with consecutive root planings (per quadrant at a one- to two-week interval), re-infection of a disinfected area might occur before completion of the treatment. This study examines, both clinically and microbiologically, whether a full-mouth disinfection within 24 hours sig...

    journal_title:Journal of dental research

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Quirynen M,Bollen CM,Vandekerckhove BN,Dekeyser C,Papaioannou W,Eyssen H

    更新日期:1995-08-01 00:00:00

  • Standardization and safety of alveolar bone-derived stem cell isolation.

    abstract::Cell therapies utilizing mesenchymal stem cells (MSCs) could overcome limitations of traditional treatments for reconstructing craniofacial tissues. This large-scale study explored a standardized methodology for the isolation and clinical-scale expansion of alveolar bone marrow-derived MSCs (aBMSCs). We harvested 103 ...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Mason S,Tarle SA,Osibin W,Kinfu Y,Kaigler D

    更新日期:2014-01-01 00:00:00

  • CGRP Modulates Orofacial Pain through Mediating Neuron-Glia Crosstalk.

    abstract::Calcitonin gene-related peptide (CGRP) plays a crucial role in the modulation of orofacial pain, and we hypothesized that CGRP mediated a neuron-glia crosstalk in orofacial pain. The objective of this study was to elucidate the mechanisms whereby CGRP mediated trigeminal neuron-glia crosstalk in modulating orofacial p...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Liang H,Hu H,Shan D,Lyu J,Yan X,Wang Y,Jian F,Li X,Lai W,Long H

    更新日期:2021-01-01 00:00:00

  • Tp63 in oral development, neoplasia, and autoimmunity.

    abstract::The Tp63 gene encodes for multiple isoforms of the p63 transcription factor, a member of the p53 family of proteins. Much like its more famous sibling, the biological role of p63 is quite complex, with wide-ranging effects on development, differentiation, and cellular lineage choices. The crucial function of p63 is ep...

    journal_title:Journal of dental research

    pub_type: 杂志文章,评审


    authors: Romano RA,Solomon LW,Sinha S

    更新日期:2012-02-01 00:00:00

  • Global burden of oral conditions in 1990-2010: a systematic analysis.

    abstract::The Global Burden of Disease (GBD) 2010 Study produced comparable estimates of the burden of 291 diseases and injuries in 1990, 2005, and 2010. This article reports on the global burden of untreated caries, severe periodontitis, and severe tooth loss in 2010 and compares those figures with new estimates for 1990. We u...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Marcenes W,Kassebaum NJ,Bernabé E,Flaxman A,Naghavi M,Lopez A,Murray CJ

    更新日期:2013-07-01 00:00:00

  • Stem Cell-Soluble Signals Enhance Multilumen Formation in SMG Cell Clusters.

    abstract::Saliva plays a major role in maintaining oral health. Patients with salivary hypofunction exhibit difficulty in chewing and swallowing foods, tooth decay, periodontal disease, and microbial infections. At this time, treatments for hyposalivation are limited to medications (e.g., muscarinic receptor agonists: pilocarpi...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Maruyama CL,Leigh NJ,Nelson JW,McCall AD,Mellas RE,Lei P,Andreadis ST,Baker OJ

    更新日期:2015-11-01 00:00:00

  • Eph/ephrinB mediate dental pulp stem cell mobilization and function.

    abstract::Damage to the dentin matrix investigates the proliferation and mobilization of dental progenitor cells to the injury site, the mechanisms of which are not defined. EphB receptors and ephrin-B ligands expressed within the perivascular niche of dental pulp have been implicated following tooth injury. We propose that ele...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Arthur A,Koblar S,Shi S,Gronthos S

    更新日期:2009-09-01 00:00:00

  • 17β-estradiol Induces MMP-9 and MMP-13 in TMJ Fibrochondrocytes via Estrogen Receptor α.

    abstract::Temporomandibular joint (TMJ) disorders, including degenerative TMJ disease, occur primarily in women of reproductive age. Previous studies showed elevated estrogen levels in subjects with TMJ disorders relative to controls and the presence of estrogen receptors α and β (ERα and ERβ) in TMJ fibrocartilage. Additionall...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Ahmad N,Chen S,Wang W,Kapila S

    更新日期:2018-08-01 00:00:00

  • The remineralization of fluoride-treated bovine enamel surfaces.

    abstract::Various etching methods are examined with respect to their ability to induce the surface nucleation and growth of fluorapatite from solutions of low, sustained supersaturation with respect to hydroxyapatite and fluorapatite. The constant composition kinetics method enables the rates of remineralization to be precisely...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Amjad Z,Koutsoukos PG,Nancollas GH

    更新日期:1982-09-01 00:00:00

  • Mobilization of intracellular calcium by methacholine and inositol 1,4,5-trisphosphate in rat parotid acinar cells.

    abstract::In the rat parotid acinar cell, methacholine caused an increase in [Ca2+]i as determined by quin-2 fluorescence. The increase in [Ca2+]i was initially independent of, and subsequently dependent on, the presence of extracellular Ca2+, indicating mobilization of intracellular Ca2+, as well as activation of Ca2+ entry. M...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Aub DL,Putney JW Jr

    更新日期:1987-02-01 00:00:00

  • The seeded growth of calcium phosphates. The kinetics of growth of dicalcium phosphate dihydrate on enamel, dentin, and calculus.

    abstract::The kinetics of growth of calcium phosphates on synthetic hydroxyapatite (HAP), human enamel, whole dentin, and human calculus has been studied at 37 C at pH values from 4.97 to 5.10 held constant by the pH-stat addition of base. Following an initial induction period, dicalcium phosphate dihydrate (DCPD) crystallizati...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Barone JP,Nancollas GH

    更新日期:1978-01-01 00:00:00

  • In Vivo Ca2+ Imaging of the Insular Cortex during Experimental Tooth Movement.

    abstract::Pain and discomfort are common problems for patients undergoing orthodontic treatment. We have demonstrated that cortical excitation propagation in the somatosensory and insular cortices (IC) induced by electrical stimulation of the periodontal ligament (PDL) is facilitated 1 d after experimental tooth movement (ETM)....

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Horinuki E,O'Hashi K,Kobayashi M

    更新日期:2020-10-08 00:00:00

  • Residual monomer/additive release and variability in cytotoxicity of light-curing glass-ionomer cements and compomers.

    abstract::In previous studies, light-cured glass-ionomer cements have been shown to evoke cytotoxic reactions. It was the purpose of this investigation (a) to determine the nature of the ingredients released into an aqueous medium from 2 light-cured glass-ionomer cements (GICs) and 3 compomers; (b) to evaluate the cytotoxicity ...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Geurtsen W,Spahl W,Leyhausen G

    更新日期:1998-12-01 00:00:00