MicroRNA-138 Inhibits Periodontal Progenitor Differentiation under Inflammatory Conditions.


:Inflammatory conditions as they occur during periodontal disease often result in decreased alveolar bone levels and a loss of connective tissue homeostasis. Here we have focused on the effect of microRNA-138 (miR-138) as a potential regulator of periodontal stem cells as they affect homeostasis during inflammatory conditions. Our data indicate that miR-138 was significantly upregulated in our periodontal disease animal model. Interaction of miR-138 with a predicted targeting site on the osteocalcin (OC) promoter resulted in a 3.7-fold reduction of luciferase activity in promoter assays compared with controls; and miR-138 overexpression in periodontal progenitors significantly inhibited OC (3.4-fold), Runx2 (2.8-fold), and collagen I (2.6-fold). Moreover, treatment with inflammatory modulators such as interleukin (IL)-6 and lipopolysaccharide (LPS) resulted in a significant 2.2-fold (IL-6) or 1.9-fold (LPS) increase in miR-138 expression, while OC and Runx2 expression was significantly decreased as a result of treatment with each inflammatory mediator. Further defining the role of miR-138 in the OC-mediated control of mineralization, we demonstrated that the LPS-induced downregulation of OC expression was partially reversed after miR-138 knockdown. LPS, miR-138 mimic, and OC small interfering RNA inhibited osteoblast differentiation marker alkaline phosphatase activity, while miR-138 inhibitor and OC protein addition enhanced alkaline phosphatase activity. Supporting the role of OC as an essential modulator of osteoblast differentiation, knockdown of miR-138 or addition of OC protein partially rescued alkaline phosphatase activity in periodontal ligament (PDL) cells subjected to LPS treatment. Our data establish miR-138 inhibitor as a potential therapeutic agent for the prevention of the bone loss associated with advanced periodontal disease.


J Dent Res


Zhou X,Luan X,Chen Z,Francis M,Gopinathan G,Li W,Lu X,Li S,Wu C,Diekwisch TG




Has Abstract


2016-02-01 00:00:00














  • Dentin biomodification potential depends on polyphenol source.

    abstract::Although proanthocyanidins (PACs) modify dentin, the effectiveness of different PAC sources and the correlation with their specific chemical composition are still unknown. This study describes the chemical profiling of natural PAC-rich extracts from 7 plants using ultra high pressure/performance liquid chromatography ...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Aguiar TR,Vidal CM,Phansalkar RS,Todorova I,Napolitano JG,McAlpine JB,Chen SN,Pauli GF,Bedran-Russo AK

    更新日期:2014-04-01 00:00:00

  • Site-specific expression of collagen I and XII mRNAs in the rat periodontal ligament at two developmental stages.

    abstract::In mammals, the periodontal ligament (PDL) is a highly specialized tissue which facilitates tooth eruption and lends mechanical support to the tooth once in occlusion. The PDL extracellular matrix fibers play a major role in such functions. During its development, the spatial arrangement of the PDL extracellular matri...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Karimbux NY,Rosenblum ND,Nishimura I

    更新日期:1992-07-01 00:00:00

  • CD73 Controls Extracellular Adenosine Generation in the Trigeminal Nociceptive Nerves.

    abstract::Purinergic signaling is involved in pain generation and modulation in the nociceptive sensory nervous system. Adenosine triphosphate (ATP) induces pain via activation of ionotropic P2X receptors while adenosine mediates analgesia via activation of metabotropic P1 receptors. These purinergic signaling are determined by...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Liu X,Ma L,Zhang S,Ren Y,Dirksen RT

    更新日期:2017-06-01 00:00:00

  • Surface integrity governs the proteome of hypomineralized enamel.

    abstract::Growing interest in the treatment and prevention of Molar/Incisor Hypomineralization (MIH) warrants investigation into the protein composition of hypomineralized enamel. Hypothesizing abnormality akin to amelogenesis imperfecta, we profiled proteins in hypomineralized enamel from human permanent first molars using a b...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Mangum JE,Crombie FA,Kilpatrick N,Manton DJ,Hubbard MJ

    更新日期:2010-10-01 00:00:00

  • X-ray diffraction study of acid-degradable glasses.

    abstract::The composition of the degradable glasses used in commercial dental glass-ionomer cements determines their leaching behavior and hence the properties of the cement. The objective of the present study was to assess if the composition and leaching in acetic acid solutions are reflected in the x-ray diffraction character...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: De Maeyer EA,Verbeeck RM

    更新日期:2001-08-01 00:00:00

  • Damage maps of veneered zirconia under simulated mastication.

    abstract::Zirconia-based restorations often fracture from chipping and/or delamination of the porcelain veneers. We hypothesized that veneer chipping/delamination is a result of the propagation of near-contact-induced partial cone cracks on the occlusal surface under mastication. Masticatory loading involves the opposing tooth ...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Kim JW,Kim JH,Janal MN,Zhang Y

    更新日期:2008-12-01 00:00:00

  • Orthodontic force stimulates eNOS and iNOS in rat osteocytes.

    abstract::Mechanosensitive osteocytes are essential for bone remodeling. Nitric oxide, an important regulator of bone remodeling, is produced by osteocytes through the activity of constitutive endothelial nitric oxide synthase (eNOS) or inducible nitric oxide synthase (iNOS). We hypothesized that these enzymes regulate the tiss...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Tan SD,Xie R,Klein-Nulend J,van Rheden RE,Bronckers AL,Kuijpers-Jagtman AM,Von den Hoff JW,Maltha JC

    更新日期:2009-03-01 00:00:00

  • Ihh signaling regulates mandibular symphysis development and growth.

    abstract::Symphyseal secondary cartilage is important for mandibular development, but the molecular mechanisms underlying its formation remain largely unknown. Here we asked whether Indian hedgehog (Ihh) regulates symphyseal cartilage development and growth. By embryonic days 16.5 to 18.5, Sox9-expressing chondrocytes formed wi...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Sugito H,Shibukawa Y,Kinumatsu T,Yasuda T,Nagayama M,Yamada S,Minugh-Purvis N,Pacifici M,Koyama E

    更新日期:2011-05-01 00:00:00

  • In vivo bioreactors for mandibular reconstruction.

    abstract::Large mandibular defects are difficult to reconstruct with good functional and aesthetic outcomes because of the complex geometry of craniofacial bone. While the current gold standard is free tissue flap transfer, this treatment is limited in fidelity by the shape of the harvested tissue and can result in significant ...

    journal_title:Journal of dental research

    pub_type: 杂志文章,评审


    authors: Tatara AM,Wong ME,Mikos AG

    更新日期:2014-12-01 00:00:00

  • Adenoviral-mediated gene transfer to mouse salivary glands.

    abstract::Adenoviral vectors effectively transfer genes to rat salivary glands. However, potent immune responses limit their use in vivo. Mice offer more opportunities than rats for the study of these immune processes. We first established conditions for infection of mouse salivary glands, with an adenoviral vector. The effects...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Wang S,Baum BJ,Yamano S,Mankani MH,Sun D,Jonsson M,Davis C,Graham FL,Gauldie J,Atkinson JC

    更新日期:2000-02-01 00:00:00

  • The effect of adsorption on the acid production of caries and noncaries-producing streptococci.

    abstract::Eighteen different Streptococcus strains including S mutans, sanguis, mitis, salivarius, bovis, and 2F2, were assayed for their relative adsorption to synthetic hydroxyapatite and for quantities of lactic acid produced while in the adsorbed state. The results indicated that all S mutans and some S sanguis have an enha...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Berry CW,Henry CA

    更新日期:1977-10-01 00:00:00

  • Oral epithelial overexpression of IL-1alpha causes periodontal disease.

    abstract::Periodontal disease is a bacterial infection that results in inflammatory destruction of tissues that support the teeth, including connective tissue and bone. In this study, we report that transgenic mice that overexpress the 17-kDa form of IL-1alpha in the basal layer of oral mucosal epithelium develop a syndrome tha...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Dayan S,Stashenko P,Niederman R,Kupper TS

    更新日期:2004-10-01 00:00:00

  • Adherence of Streptococcus sanguis to salivary mucin bound to glass.

    abstract::This study demonstrated that human submandibular-sublingual saliva (HSMSL) provided a better substrate than did whole saliva or parotid saliva for the binding of Streptococcus sanguis in a glass adherence assay. Additional evidence indicated that the lower molecular weight salivary mucin in HSMSL was involved in these...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Stinson MW,Levine MJ,Cavese JM,Prakobphol A,Murray PA,Tabak LA,Reddy MS

    更新日期:1982-12-01 00:00:00

  • Impact of Maintenance Therapy for the Prevention of Peri-implant Diseases: A Systematic Review and Meta-analysis.

    abstract::At the present time, peri-implantitis has become a global burden that occurs with a frequency from 1% to 47% at implant level. Therefore, we aimed herein at assessing the impact of peri-implant maintenance therapy (PIMT) on the prevention of peri-implant diseases. Electronic and manual literature searches were conduct...

    journal_title:Journal of dental research

    pub_type: 杂志文章,meta分析,评审


    authors: Monje A,Aranda L,Diaz KT,Alarcón MA,Bagramian RA,Wang HL,Catena A

    更新日期:2016-04-01 00:00:00

  • Mechanical significance of the trabecular microstructure of the human mandibular condyle.

    abstract::The human mandibular condyle has a parasagittal plate-like trabecular structure. We tested the hypothesis that this structure reflects the mechanical loading of the condyle. We developed a finite element model of the condyle to analyze the strains occurring during static compressive loading. The principal strains in t...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: van Ruijven LJ,Giesen EB,van Eijden TM

    更新日期:2002-10-01 00:00:00

  • Effect of facial sensory re-training on sensory thresholds.

    abstract::Nearly 100% of patients experience trauma to the trigeminal nerve during orthognathic surgery, impairing sensation and sensory function on the face. In a recent randomized clinical trial, people who performed sensory re-training exercises reported less difficulty related to residual numbness and decreased lip sensitiv...

    journal_title:Journal of dental research

    pub_type: 杂志文章,多中心研究,随机对照试验


    authors: Essick GK,Phillips C,Zuniga J

    更新日期:2007-06-01 00:00:00

  • The effect of dental amalgams on mercury levels in expired air.

    abstract::The expired air of a group of 48 persons, 40 with and eight without dental amalgam restorations, was analyzed for its mercury content before and after chewing. Expired air samples were collected in polyethylene bags, and a known quantity of each was pumped into the mercury detector for measurement. The results showed ...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Svare CW,Peterson LC,Reinhardt JW,Boyer DB,Frank CW,Gay DD,Cox RD

    更新日期:1981-09-01 00:00:00

  • Topical Fluoride to Prevent Root Caries: Systematic Review with Network Meta-analysis.

    abstract::The aim of this systematic review and network meta-analysis was to summarize the direct and indirect clinical evidence on the effectiveness of professionally applied and self-applied topical fluorides in preventing dental root caries. Controlled clinical trials with any follow-up duration were included. MEDLINE, PubMe...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Zhang J,Sardana D,Li KY,Leung KCM,Lo ECM

    更新日期:2020-05-01 00:00:00

  • The effects of xylitol-containing chewing gums on dental plaque and acidogenic potential.

    abstract::The aim of this study was to test the hypothesis that the chewing of xylitol- or xylitol/sorbitol-containing chewing gum reduces plaque formation and the acidogenic potential of dental plaque. Thirty healthy volunteers aged from 19 to 28 yrs were randomly allocated to one of three test groups, chewing either xylitol-,...

    journal_title:Journal of dental research

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Scheie AA,Fejerskov O,Danielsen B

    更新日期:1998-07-01 00:00:00

  • Survival and Complications of Single Dental Implants in the Edentulous Mandible Following Immediate or Delayed Loading: A Randomized Controlled Clinical Trial.

    abstract::It was the aim of this 24-mo randomized controlled clinical trial to investigate whether the survival of a single median implant placed in the edentulous mandible to retain a complete denture is not compromised by immediate loading. Secondary outcomes were differences in prosthetic complications between the loading pr...

    journal_title:Journal of dental research

    pub_type: 杂志文章,多中心研究,随机对照试验


    authors: Kern M,Att W,Fritzer E,Kappel S,Luthardt RG,Mundt T,Reissmann DR,Rädel M,Stiesch M,Wolfart S,Passia N

    更新日期:2018-02-01 00:00:00

  • Graphene Oxide Enables the Reosteogenesis of Previously Contaminated Titanium In Vitro.

    abstract::The main goal of peri-implantitis treatment is to control infection and arrest bone loss, which requires the removal of polymicrobial biofilms on the implant surface and the reduction of tissue invasion. Additionally, prognosis can be improved if reosseointegration occurs on previously contaminated implants. To evalua...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Qin W,Wang C,Jiang C,Sun J,Yu C,Jiao T

    更新日期:2020-07-01 00:00:00

  • Molecular and cellular mechanisms that lead to Candida biofilm formation.

    abstract::Fungal infections in the oral cavity are mainly caused by C. albicans, but other Candida species are also frequently identified. They are increasing in prevalence, especially in denture-wearers and aging people, and may lead to invasive infections, which have a high mortality rate. Attachment to mucosal tissues and to...

    journal_title:Journal of dental research

    pub_type: 杂志文章,评审


    authors: ten Cate JM,Klis FM,Pereira-Cenci T,Crielaard W,de Groot PW

    更新日期:2009-02-01 00:00:00

  • The effect of cavity preparation on the metabolism of protein in dentin.

    abstract::Tritiated proline was administered to domestic cats during the development of their permanent dentition. The amount of residual radioactivity was determined autoradiographically at various times after cavity preparation in mature molars and premolars. Two wk after cavity preparation, the amount of radiolabel beneath t...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Hoppenbrouwers PM,Borggreven JM,Thé GS,Maltha JC

    更新日期:1982-09-01 00:00:00

  • Role of Oxidized Lipids and TRP Channels in Orofacial Pain and Inflammation.

    abstract::Acute or chronic inflammation comprises a highly prevalent type of orofacial pain and is mediated by the generation of endogenous agonists that activate numerous receptors expressed on terminals of trigeminal (TG) nociceptive afferent neurons. One such studied receptor is transient receptor potential vanilloid subtype...

    journal_title:Journal of dental research

    pub_type: 杂志文章,评审


    authors: Hargreaves KM,Ruparel S

    更新日期:2016-09-01 00:00:00

  • Purification, characterization, and cloning of enamel matrix serine proteinase 1.

    abstract::The maturation of dental enamel succeeds the degradation of organic matrix. Inhibition studies have shown that this degradation is accomplished by a serine-type proteinase. To isolate and characterize cDNA clones encoding this proteinase, we used two degenerate primer approaches to amplify part of the coding region us...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Simmer JP,Fukae M,Tanabe T,Yamakoshi Y,Uchida T,Xue J,Margolis HC,Shimizu M,DeHart BC,Hu CC,Bartlett JD

    更新日期:1998-02-01 00:00:00

  • Morphologic and biochemical changes of the masseter muscles induced by occlusal wear: studies in a rat model.

    abstract::Occlusal alterations may result in changes in the functional performance of masticatory muscles. In this study, we set up an experimental model in rats to examine whether masticatory muscle abnormalities occur after a malocclusion is induced. Rats underwent unilateral amputation of the molar cusps to simulate an occlu...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Bani D,Bani T,Bergamini M

    更新日期:1999-11-01 00:00:00

  • Solvent effects on bonding organo-silane to silica surfaces.

    abstract::Interfacial bonding and stability of gamma-methacryloxypropyltrimethoxysilane with silica surfaces have been studied by means of infrared spectroscopy. The addition of n-propylamine enhances silanization of gamma-methacryloxypropyltrimethoxysilane to silica surfaces in normal aliphatic hydrocarbons, and cyclohexane yi...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Chen TM,Brauer GM

    更新日期:1982-12-01 00:00:00

  • Kinin generation in the gingival inflammatory response to topically applied bacterial lipopolysaccharides.

    abstract::A water-soluble lipopolysaccharide from Salmonella enteritidis and a phenol-soluble lipopolysaccharide from Leptotrichia buccalis were applied topically to the healthy marginal gingivae of beagle dogs. Saline was applied to contralateral areas as an internal control. Increases in vascular permeability were monitored b...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Montgomery EH,White RR

    更新日期:1986-02-01 00:00:00

  • The formation of hydroxyapatite-ionomer cements at 38 degrees C.

    abstract::This study describes the formation of a calcium polyacrylate-hydroxyapatite cement. Our hypothesis was that calcium phosphates would rapidly hydrolyze in the presence of polyacrylic acid (PAA) to form a cement. PAA, tetracalcium phosphate (TetCP), and dicalcium phosphate (DCP) were reacted together and formed calcium ...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: TenHuisen KS,Brown PW

    更新日期:1994-03-01 00:00:00

  • Experimental observation of silver and gold penetration into dental ceramic by means of a radiotracer technique.

    abstract::A radiotracer technique was used to study silver and gold diffusion into dental porcelain under experimental conditions close to the real conditions in prosthetic laboratories for porcelain bakes. It was clearly shown that these non-oxidizable elements were able to diffuse into the ceramic as well as oxidizable ones. ...

    journal_title:Journal of dental research

    pub_type: 杂志文章


    authors: Moya F,Payan J,Bernardini J,Moya EG

    更新日期:1987-12-01 00:00:00