Streptococcus salivarius promotes mucin putrefaction and malodor production by Porphyromonas gingivalis.

Abstract:

:Although the contribution of the oral microbiota to oral malodor is well-documented, the potential role of Gram-positive micro-organisms is unclear. In the current study, we tested the hypothesis that Gram-positive micro-organisms contribute to malodor production by deglycosylating oral glycoproteins, rendering them susceptible to subsequent proteolysis. To this end, we examined the effect of Streptococcus salivarius on Porphyromonas gingivalis-mediated putrefaction of a model glycoprotein (pig gastric mucin). Malodor was scored by two odor judges, and volatile sulfides were determined with the use of a sulfide monitor. Mucin degradation was followed by electrophoresis on SDS-PAGE. Results showed that the addition of S. salivarius or beta-galactosidase promoted mucin degradation and concomitant malodor production. Addition of glycosidic inhibitors (p-APTG and glucose) inhibited this process. These results suggest that Gram-positive micro-organisms such as S. salivarius contribute to oral malodor production by deglycosylating salivary glycoproteins, thus exposing their protein core to further degradation by Gram-negative micro-organisms.

journal_name

J Dent Res

authors

Sterer N,Rosenberg M

doi

10.1177/154405910608501007

subject

Has Abstract

pub_date

2006-10-01 00:00:00

pages

910-4

issue

10

eissn

0022-0345

issn

1544-0591

pii

85/10/910

journal_volume

85

pub_type

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