Abstract:
:Squamous cell carcinoma (SCC) of the head and neck is the sixth most frequent cancer worldwide. The survival rate is among the lowest of the major cancers and has not improved significantly in the past two decades. Extensive local invasion and regional lymph node metastasis are, in large part, responsible for the poor clinical outcome of these tumors. Keratin intermediate filaments are the most abundant cytoskeletal proteins in SCCs and regulate the migration of normal and transformed epithelial cells. Previous studies have shown that expression of the 40-kDa keratin K19 is dysregulated in SCCs arising from oral epithelium. Immunohistochemical experiments demonstrated that, while normal epithelium and dysplastic lesions expressed abundant K19 protein, invasive SCCs exhibited a patchy or negative staining pattern. We subsequently determined that K19 expression was consistently downregulated in seven SCC lines compared with normal epithelium. We therefore wanted to determine if K19 downregulation affected the invasive phenotype of these cells. We found that SCC lines which do not express K19 are significantly more invasive in vitro than those which retain expression of this gene. Stable expression of the K19 cDNA in K19 negative cell lines altered cell morphology and intercellular adhesiveness, and significantly decreased the number of cells able to migrate through a reconstituted basement membrane. Reduced invasiveness was not due to decreased metalloproteinase activity in the K19-expressing clones. We conclude that K19 overexpression in oral SCCs decreases their invasive potential by diminishing migratory capability.
journal_name
J Dent Resjournal_title
Journal of dental researchauthors
Crowe DL,Milo GE,Shuler CFdoi
10.1177/00220345990780061001keywords:
subject
Has Abstractpub_date
1999-06-01 00:00:00pages
1256-63issue
6eissn
0022-0345issn
1544-0591journal_volume
78pub_type
杂志文章abstract::Clinical evidence is lacking regarding the influence of the amount of residual coronal dentin and of post placement on the failure risk of endodontically compromised teeth. The aim of this prospective clinical trial was to assess whether these factors significantly affect the two-year survival of restored pulpless pre...
journal_title:Journal of dental research
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abstract::Macrophage colony-stimulating factor (CSF-1) is a key regulatory cytokine for amelogenesis, and ameloblasts synthesize CSF-1. We hypothesized that PDGF stimulates DNA synthesis and regulates CSF-1 in these cells. We determined the effect of PDGF on CSF-1 expression using MEOE-3M ameloblasts as a model. By RT-PCR, MEOE...
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doi:10.1177/154405910808700105
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abstract::These studies defined the normal and hormonally altered development of activity for tyrosine hydroxylase (T-OH), the rate-limiting enzyme in catecholamine biosynthesis (Levitt et al., 1965), and choline acetyltransferase activity (CAT) in the male rat superior cervical ganglion (SCG). Additionally, salivary gland weig...
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doi:10.1177/00220345870660030901
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abstract::Growth factors such as epidermal growth factor (EGF) and transforming growth factor-alpha (TGF-alpha) play an important role in cell proliferation during embryogenesis. The purposes of the study were to characterize the expression patterns of EGF and TGF-alpha and their receptor, EGF receptor (EGF-R), and to analyze r...
journal_title:Journal of dental research
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更新日期:1996-08-01 00:00:00
abstract::Mutations in ALPL result in hypophosphatasia (HPP), a disease causing defective skeletal mineralization. ALPL encodes tissue nonspecific alkaline phosphatase (ALP), an enzyme that promotes mineralization by reducing inorganic pyrophosphate, a mineralization inhibitor. In addition to skeletal defects, HPP causes dental...
journal_title:Journal of dental research
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journal_title:Journal of dental research
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journal_title:Journal of dental research
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abstract::Understanding the mechanical properties of human teeth is important to clinical tooth preparation and to the development of "tooth-like" restorative materials. Previous studies have focused on the macroscopic fracture behavior of enamel and dentin. In the present study, we performed indentation studies to understand t...
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abstract::Limited cure depth is a drawback of light-activated composites. We hypothesize that curing light transmission and cure depth are influenced by monomer reactivity and filler/resin refractive index mismatch. Light transmission throughout cure was recorded for composites based on strontium (refractive index 1.51) or bari...
journal_title:Journal of dental research
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abstract::The goal of the present investigation was to study whether periodic use of fluoride-gel treatments resulted in an increased prevalence of dental fluorosis. 269 children, from 14 to 16 years of age, who had received semi-annual or quarterly fluoride-gel treatments were examined for possible fluoride-induced enamel chan...
journal_title:Journal of dental research
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abstract::Cellular hypoxia may be a useful indication of tissue distress in the dental pulp that could be used to investigate the early stages of pulpal responses. Tritiated misonidazole (3H-MISO) is a marker which preferentially labels cells with decreased oxygen tension (hypoxia). The experiments reported here were carried ou...
journal_title:Journal of dental research
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更新日期:1996-10-01 00:00:00
abstract::The purpose of this study was to determine the surface roughness of enamel-to-enamel contact areas in order to provide a standard for comparison with surface characteristics of commercially available composite restorative materials. In addition, the inherent surface roughness of resin composites was evaluated profilom...
journal_title:Journal of dental research
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更新日期:1991-09-01 00:00:00
abstract::Eighteen different Streptococcus strains including S mutans, sanguis, mitis, salivarius, bovis, and 2F2, were assayed for their relative adsorption to synthetic hydroxyapatite and for quantities of lactic acid produced while in the adsorbed state. The results indicated that all S mutans and some S sanguis have an enha...
journal_title:Journal of dental research
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doi:10.1177/00220345770560101401
更新日期:1977-10-01 00:00:00
abstract::The discovery of the phenomenon "osseointegration," or functional ankylosis, has led to the development of oral implants with high clinical performance. Consequently, the placement of titanium implants has changed the paradigms of restorative dentistry. Implants are used to prevent placing reconstructions anchored on ...
journal_title:Journal of dental research
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abstract::Stable fluoride-resistant mutants of Streptococcus mutans GS-5 were isolated on Todd-Hewitt agar by a step-wise selection procedure. Resistance is defined here as the ability to form colonies in 48 hr. First-step mutants demonstrated six different levels of resistance, ranging from 400 to 1000 micrograms/mL sodium flu...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345870660101701
更新日期:1987-10-01 00:00:00
abstract::Periodontitis is a chronic inflammatory disease affecting oral tissues. The continuous, high production of cytokines by host cells triggered by periodontopathogens is thought to be responsible for the destruction of tooth-supporting tissues. Macrophages play a critical role in this host inflammatory response to period...
journal_title:Journal of dental research
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journal_title:Journal of dental research
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更新日期:2014-02-01 00:00:00
abstract::Greater fluoride concentrations were obtained in enamel treated with phosphate/fluoride solutions than in that treated with TiF4 at similar pH's (1.0) and F concentrations (0.6M F and 1.6M F). However, fluoride solution without added phosphate at pH 1.0 and 1.6M F concentration produced lower fluoride concentrations i...
journal_title:Journal of dental research
pub_type: 杂志文章
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更新日期:1979-02-01 00:00:00
abstract::As the use of adhesive restorative materials has increased during the last several years, interest in adhesive materials that release fluoride has also grown. The purpose of this study was to measure fluoride release from several adhesive restorative materials and to evaluate its effect on dentin resistance to deminer...
journal_title:Journal of dental research
pub_type: 杂志文章
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更新日期:1999-10-01 00:00:00
abstract::Several studies indicated that serotype/biotype c strains are the most common on a global basis, but that regional differences may occur relative to other serotype/biotypes. Of particular interest is the observation that individuals residing in the Middle East have higher incidences of serotype e and d strains relativ...
journal_title:Journal of dental research
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更新日期:1984-01-01 00:00:00
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journal_title:Journal of dental research
pub_type: 杂志文章
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更新日期:1987-06-01 00:00:00
abstract::Biological mediators have been used to enhance periodontal regeneration. The aim of this prospective randomized controlled study was to evaluate the safety and effectiveness of 3 doses of fibroblast growth factor 2 (FGF-2) when combined with a β-tricalcium phosphate (β-TCP) scaffold carrier placed in vertical infrabon...
journal_title:Journal of dental research
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abstract::Contact angle measurements on layers of bacteria were used to determine the bacterial surface free energy (gamma b) of a variety of oral streptococcal strains, both without and after being coated with human whole saliva. At least four isolates of each species, either freshly isolated or laboratory strains, were used. ...
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abstract::Stem cells from exfoliated deciduous teeth (SHED) possess multipotent differentiation and immunomodulatory properties. They have been used for orofacial bone regeneration and autoimmune disease treatment. In this study, we show that acetylsalicylic acid (ASA) treatment is able to significantly improve SHED-mediated os...
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abstract::The temporomandibular joint (TMJ) undergoes degenerative changes among patients who suffer from arthritis, and yet the pathogenesis of TMJ osteoarthritis and rheumatoid arthritis is poorly understood. We hypothesized that sustained inflammation in the TMJ induces structural abnormalities, and accordingly characterized...
journal_title:Journal of dental research
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更新日期:2012-05-01 00:00:00