Abstract：
:The major phosphoprotein in dentin is the aspartic acid and serine-rich protein called dentin phosphophoryn (DPP). DPP appears to be synthesized as a part of a larger compound protein, dentin sialophosphoprotein (DSPP). DSPP has never been isolated or detected in dentin extracts. It is now evident that DSPP is a chimeric protein composed of 3 parts: dentin sialoprotein (DSP), DPP, and dentin glycoprotein (DGP). Previous reports have suggested that the BMP1 protease is responsible for processing DSPP. However, unequal amounts of these products are present in the dentin matrix. Here, we provide evidence for an internal ribosome entry site in the DSPP gene that directs the synthesis of DPP. This mechanism would account for unequal amounts of intracellular DSP and DPP. The internal ribosomal entry site (IRES) activity varied in different cell types, suggesting the presence of additional regulatory elements during the translational regulation of DPP. Further, we provide evidence that DPP is transported to the extracellular matrix (ECM) through exosomes. Using tissue recombination and lentivirus-mediated gain-of-function approaches, we also demonstrate that DPP is essential for the formation of well-defined tooth structures with mineralized dentin matrix.
journal_name
J Dent Resjournal_title
Journal of dental researchauthors
Zhang Y,Song Y,Ravindran S,Gao Q,Huang CC,Ramachandran A,Kulkarni A,George Adoi
10.1177/0022034513516631subject
Has Abstractpub_date
2014-02-01 00:00:00pages
155-61issue
2eissn
0022-0345issn
1544-0591pii
0022034513516631journal_volume
93pub_type
杂志文章abstract：:Inflammatory conditions as they occur during periodontal disease often result in decreased alveolar bone levels and a loss of connective tissue homeostasis. Here we have focused on the effect of microRNA-138 (miR-138) as a potential regulator of periodontal stem cells as they affect homeostasis during inflammatory con...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/0022034515613043
更新日期：2016-02-01 00:00:00
abstract：:Mixtures containing two bacterial species were analyzed using flow cytometric techniques. Light scattering characteristics of Streptococcus mutans and Actinomyces viscosus show unique profiles for pure cultures. However, the light scatter analysis of a mixture containing these two species demonstrates overlapping near...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345840630080501
更新日期：1984-08-01 00:00:00
abstract：:This study aims at modifying dual-cure composite cements by adding thio-urethane oligomers to improve mechanical properties, especially fracture toughness, and reduce polymerization stress. Thiol-functionalized oligomers were synthesized by combining 1,3-bis(1-isocyanato-1-methylethyl)benzene with trimethylol-tris-3-m...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/0022034514551768
更新日期：2014-12-01 00:00:00
abstract：:Extracellular microcapsules have been demonstrated on cells of most serotypes of Streptococcus mutans by electron microscopy, using bacterial strains of the various serotypes and peroxidase labeled or unlabeled immune serum. A correlation was noted between the amount of capsular substance on the strains of S mutans ex...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345770560021101
更新日期：1977-02-01 00:00:00
abstract：:The Wingless/integrase-1 (Wnt) family of protein ligands and their functional antagonists, secreted frizzled-related proteins (sFRPs), regulate various biological processes ranging from embryonic development to immunity and inflammation. Wnt5a and sFRP5 comprise a typical ligand/antagonist pair, and the former molecul...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/0022034516687248
更新日期：2017-05-01 00:00:00
abstract：:While dental pulp appears to be able to form mineralized matrices that do not always resemble dentin, the precise characteristics of the hard tissue and the mechanism of its induction remain unknown. Therefore, we evaluated hard tissue induced by transplantation of pulp into subcutaneous tissue. Seven days after trans...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/154405910708600515
更新日期：2007-05-01 00:00:00
abstract：:Growing evidence suggests that individual genetic susceptibility may influence the host's response to infections. The aim of this project was to study whether gene polymorphisms of inflammatory markers are associated with the presence of viable periodontopathogenic bacteria. We extracted genomic DNA from 45 young adul...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/154405910708600505
更新日期：2007-05-01 00:00:00
abstract：:Khat-chewing has been associated with oral lesions including oral cancer, but the mechanisms leading to their development are not known. We hypothesized that khat interferes with the physiological processes of the oral mucosa, such as cell proliferation and differentiation, and aimed at investigating the effects of kh...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/0022034509354980
更新日期：2010-03-01 00:00:00
abstract：:The biocompatibility of two implant materials, vitallium and poly-methyl methacrylate (PMMA), was evaluated in an animal model in which bone formation and resorption are independent of each other. Neither material accelerated resorption, but formation was inhibited 12% by vitallium and 38% by PMMA. When three PMMA pre...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345810600010801
更新日期：1981-01-01 00:00:00
abstract：:Resin luting of all-ceramic restorations results in increased performance; however, the strengthening mechanism and the role of the mechanical properties of the resin are not fully understood. The hypothesis tested is that ceramic strength enhancement is dependent on the elastic modulus of the resin. Three-point flexu...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/154405910708600606
更新日期：2007-06-01 00:00:00
abstract：:Genetic approaches are rapidly yielding new information about our sense of taste. This information comes from both molecular studies of genes encoding taste receptors and other taste-signaling components, and from studies of inherited variation in taste abilities. Our understanding of bitter taste has advanced by comb...
journal_title：Journal of dental research
pub_type： 杂志文章,评审
doi：10.1177/154405910408300603
更新日期：2004-06-01 00:00:00
abstract：:The oral microbiota was compared between Romanian adolescents with a high prevalence of caries and no dental care and Swedish caries-active and caries-free adolescents in caries prevention programs and with a low prevalence of caries. Biofilm samples were analyzed by FLX+ pyrosequencing of the V1 to V4 hypervariable r...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/0022034515609554
更新日期：2016-01-01 00:00:00
abstract：:The treatment of periodontitis/peri-implantitis involves the reduction/eradication of periopathogens. After therapy, beneficial and pathogenic species recolonize the subgingival area. The dynamics of recolonization and especially the role of the supragingival environment in this process are still not well-understood. ...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/154405910508400409
更新日期：2005-04-01 00:00:00
abstract：:Non-steroidal anti-inflammatory drugs (NSAIDs) are used to manage pain and inflammatory disorders. We hypothesized that gingival fibroblasts actively accumulate NSAIDs and enhance their levels in gingival connective tissue. Using fluorescence to monitor NSAID transport, we demonstrated that cultured gingival fibroblas...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/154405910608500511
更新日期：2006-05-01 00:00:00
abstract：:The purpose of this study was to determine the surface roughness of enamel-to-enamel contact areas in order to provide a standard for comparison with surface characteristics of commercially available composite restorative materials. In addition, the inherent surface roughness of resin composites was evaluated profilom...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345910700091301
更新日期：1991-09-01 00:00:00
abstract：:Decisions to replace existing restorations are often based on clinical findings of margin discrepancies and other restoration defects. Previous studies have suggested that such findings do not correlate well with the actual presence of secondary caries, and that treatment should be deferred until caries is clinically ...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345930720121301
更新日期：1993-12-01 00:00:00
abstract：:Investigators have used chemotherapeutic agents topically for plaque control without knowing the drug concentration necessary to inhibit the growth of odontopathic microorganisms. S mutans, S sanguis, A viscosus and A naeslundii are important components of the plaque flora. The minimal inhibitory concentrations (MIC) ...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345760550040401
更新日期：1976-07-01 00:00:00
abstract：:The pathomechanics of degenerative joint disease of the temporomandibular joint (TMJ) may involve fatigue produced by mechanical work on the articulating tissues. This study tested the hypotheses that mechanical work in the TMJ (i) varies with the type of mandibular activity, and (ii) is evenly distributed over TMJ su...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/154405910608501106
更新日期：2006-11-01 00:00:00
abstract：:This study demonstrated that human submandibular-sublingual saliva (HSMSL) provided a better substrate than did whole saliva or parotid saliva for the binding of Streptococcus sanguis in a glass adherence assay. Additional evidence indicated that the lower molecular weight salivary mucin in HSMSL was involved in these...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345820610120101
更新日期：1982-12-01 00:00:00
abstract：:The balance between bone resorption and bone formation is vital for maintenance and regeneration of alveolar bone and supporting structures around teeth and dental implants. Tissue regeneration in the oral cavity is regulated by multiple cell types, signaling mechanisms, and matrix interactions. A goal for periodontal...
journal_title：Journal of dental research
pub_type： 杂志文章,评审
doi：10.1177/0022034515618887
更新日期：2016-03-01 00:00:00
abstract：:During orofacial tissue development, the anterior and posterior regions of the Meckel's cartilage undergo mineralization, while the middle region undergoes degeneration. Despite the interesting and particular phenomena, the mechanisms that regulate the different fates of Meckel's cartilage, including the effects of bi...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/0022034520960118
更新日期：2021-02-01 00:00:00
abstract：:Overgrowth of oral yeast is a common problem among the elderly. Probiotic bacteria are known to inhibit the growth of pathogenic microbes. We tested the hypothesis that cheese containing probiotic bacteria can reduce the prevalence of oral Candida. During this 16-week, randomized, double-blind, placebo-controlled stud...
journal_title：Journal of dental research
pub_type： 杂志文章,随机对照试验
doi：10.1177/154405910708600204
更新日期：2007-02-01 00:00:00
abstract：:Oxygen inhibits free radical polymerization and yields polymers with uncured surfaces. This is a concern when thin layers of resin are being polymerized, or in circumstances where conventional means of eliminating inhibition are inappropriate. In this study, we tested the hypothesis that viscosity, filler content, and...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/154405910508400808
更新日期：2005-08-01 00:00:00
abstract：:Six subjects wore intra-oral devices carrying eight bovine enamel blocks which were covered with a layer of S. mutans. They rinsed their mouths for five sec, one min, or multiples of one min with a 5 or 10% glucose (G) solution. Demineralization was measured after 45 min by determining the change in iodide permeabilit...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345860650021001
更新日期：1986-02-01 00:00:00
abstract：:The tissue-implant interfaces of functional and non-functional endosseous dental implants were compared histologically for up to one year post-operatively. Nonmineralized connective tissue zones (a "fibrous capsule") existed in all functional interfaces. Direct, or nearly direct, bone apposition to implants occurred i...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345790580100201
更新日期：1979-10-01 00:00:00
abstract：:Diet is recognized as one of the most important factors affecting the equilibrium of the oral bacterial flora. Studies related to dental caries development have focused mainly on the effect of carbohydrates. In the present work, using swabbing coupled with an immuno-colony-blot assay, we followed the indigenous oral b...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345900690030801
更新日期：1990-03-01 00:00:00
abstract：:Eighteen different Streptococcus strains including S mutans, sanguis, mitis, salivarius, bovis, and 2F2, were assayed for their relative adsorption to synthetic hydroxyapatite and for quantities of lactic acid produced while in the adsorbed state. The results indicated that all S mutans and some S sanguis have an enha...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345770560101401
更新日期：1977-10-01 00:00:00
abstract：:During the polymerization of multifunctional monomers for dental restorations, typical final double-bond conversions range from 55 to 75%. The low conversion results in a large amount of extractable monomer, reduced adhesion to the filler, and the potential for increased swelling. In this work, the ability to increase...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345960750081301
更新日期：1996-08-01 00:00:00
abstract：:In order for the role of parotid amylase in starch digestion in the gastro-intestinal tracts of diabetic rats to be clarified, this study investigated the effect of parotid-duct ligation on both amylase secretion from the parotid glands and pancreas into the gastro-intestinal tract and on starch digestion in the gastr...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345890680091501
更新日期：1989-09-01 00:00:00
abstract：:Epithelial-mesenchymal interactions are required for tooth formation. Bone morphogenetic protein 4 (Bmp4) is a crucial signaling molecule during this process. For better understanding of the role of the Bmp4 gene during tooth development, we studied the mechanisms that control its temporal and spatial expression durin...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/002203450208100103
更新日期：2002-01-01 00:00:00
