Abstract:
:Mixtures containing two bacterial species were analyzed using flow cytometric techniques. Light scattering characteristics of Streptococcus mutans and Actinomyces viscosus show unique profiles for pure cultures. However, the light scatter analysis of a mixture containing these two species demonstrates overlapping near the origin. Thus, light scatter analysis was not sufficient to speciate bacteria with different morphologies. Labeling of samples with species-specific immunofluorescent antibodies permitted speciation of mixtures. As the percentage of the bacterium to which the antibody is directed increased in a two-component mixture, fluorescent flow cytometric analysis showed a corresponding increase in the percentage of cells displaying fluorescent labeling. These methods could permit the rapid identification of bacteria from oral sites without culturing.
journal_name
J Dent Resjournal_title
Journal of dental researchauthors
Barnett JM,Cuchens MA,Buchanan Wdoi
10.1177/00220345840630080501subject
Has Abstractpub_date
1984-08-01 00:00:00pages
1040-2issue
8eissn
0022-0345issn
1544-0591journal_volume
63pub_type
杂志文章abstract::The biocompatibility of two implant materials, vitallium and poly-methyl methacrylate (PMMA), was evaluated in an animal model in which bone formation and resorption are independent of each other. Neither material accelerated resorption, but formation was inhibited 12% by vitallium and 38% by PMMA. When three PMMA pre...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345810600010801
更新日期:1981-01-01 00:00:00
abstract::To date, the precise etiology of molar-incisor hypomineralization (MIH) is uncertain. Vitamin D plays a key role in hard tissue formation. Therefore, this study aimed to analyze the relationship between serum 25-hydroxy-vitamin D (25(OH)D) status and dental health data obtained from 1,048 children in a 10-year follow-...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/0022034514561657
更新日期:2015-02-01 00:00:00
abstract::In previous studies, human dental pulp stem cells (hDPSCs) were mainly isolated from adults. In this present study, we characterized hDPSCs isolated from an earlier developmental stage to evaluate the potential usage of these cells for tissue-regenerative therapy. hDPSCs isolated at the crown-completed stage showed a ...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/154405910808700716
更新日期:2008-07-01 00:00:00
abstract::Although a secular decline is being seen in coronal caries in children in industrialized countries, there appears to be a relatively high prevalence of root caries, with chronologically older adults exhibiting the highest prevalences. This paper reviews the descriptive and analytical epidemiology of root caries as fou...
journal_title:Journal of dental research
pub_type: 杂志文章,评审
doi:10.1177/00220345900690051901
更新日期:1990-05-01 00:00:00
abstract::Understanding the mechanical properties of human teeth is important to clinical tooth preparation and to the development of "tooth-like" restorative materials. Previous studies have focused on the macroscopic fracture behavior of enamel and dentin. In the present study, we performed indentation studies to understand t...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345980770030601
更新日期:1998-03-01 00:00:00
abstract::Diet is recognized as one of the most important factors affecting the equilibrium of the oral bacterial flora. Studies related to dental caries development have focused mainly on the effect of carbohydrates. In the present work, using swabbing coupled with an immuno-colony-blot assay, we followed the indigenous oral b...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345900690030801
更新日期:1990-03-01 00:00:00
abstract::A silver-plating technique was developed in an effort to produce good mechanical bonding characteristics between stainless steel pins and amalgam. Metallographic microscope and scanning electron microscope (SEM) studies were made to assess the presence, or otherwise, of such a bond between (a) the silver layer plating...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345800590020301
更新日期:1980-02-01 00:00:00
abstract::The pellicle serves as a multifunctional protective layer, providing, e.g., lubrication and remineralization and also acting as a diffusion barrier. In addition, since the formation of the pellicle precedes the adhesion of micro-organisms, it is also important as a conditioning film. We present a novel approach to stu...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/0022034512456704
更新日期:2012-10-01 00:00:00
abstract::The relationship between the serum IgG antibody titer against seven species of Gram-negative periodontopathic bacteria and clinical parameters (including plaque index, gingival index, periodontal pocket depth, and alveolar bone loss) was studied in 38 subjects. IgG antibody titer against the sonicated antigens was det...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345850640111101
更新日期:1985-11-01 00:00:00
abstract::The benefits of early orthodontic treatment are continuously discussed, but studies are few. We examined whether definite need for orthodontic treatment could be eliminated in public health care by systematically focusing on early intervention. One age cohort living in a rural Finnish municipality (N = 85) was regular...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/154405910808700604
更新日期:2008-06-01 00:00:00
abstract::Dental caries can be described as a dysbiosis of the oral microbial community, in which acidogenic, aciduric, and acid-adapted bacterial species promote a pathogenic environment, leading to demineralization. Alkali generation by oral microbes, specifically via arginine catabolic pathways, is an essential factor in mai...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/0022034517707512
更新日期:2017-07-01 00:00:00
abstract::Focusing public insurance programs on interceptive orthodontics (IO) may increase access for low-income children. This report presents outcomes from a randomized clinical trial (RCT) comparing IO with comprehensive orthodontics (CO) in Medicaid patients. One hundred seventy pre-adolescents with Medicaid-eligible maloc...
journal_title:Journal of dental research
pub_type: 杂志文章,随机对照试验
doi:10.1177/0022034512448663
更新日期:2012-07-01 00:00:00
abstract::Bone marrow-derived mesenchymal stem/progenitor cells (BMSCs) are commonly used in regeneration therapy. The current primary source of BMSCs is the iliac crest; however, the procedure is associated with various burdens on the patient, including the risk of pain and infection. Hence, the possibility to collect BMSCs fr...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/0022034514549377
更新日期:2014-11-01 00:00:00
abstract::In a three-year study of 145 adolescent males in a boarding school in Wisconsin, there were no statistically significant differences in dental caries experience between a group consuming phosphates added to pre-sweetened and non-presweetened ready-to-eat cereals, and the control group, or between cereal eaters and non...
journal_title:Journal of dental research
pub_type: 临床试验,杂志文章
doi:10.1177/00220345790580090201
更新日期:1979-09-01 00:00:00
abstract::A double-blind randomized clinical trial was performed in 6- to 7-yr-old schoolchildren to evaluate, in a 30-mo period, whether the caries increment on the distal surface of the second primary molars adjacent to permanent first molars sealed with fluoride release compounds would be lower with respect to those adjacent...
journal_title:Journal of dental research
pub_type: 杂志文章,随机对照试验
doi:10.1177/0022034514535808
更新日期:2014-07-01 00:00:00
abstract::The incisor dentin of hypophosphatemic (Hyp) mice was examined histopathologically to determine whether the multiple occurrences of interglobular dentin would be influenced by the serum phosphate level. Both normal and Hyp mice (12 weeks of age) were divided into two groups. The mice in one group were given a control ...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345920710031101
更新日期:1992-03-01 00:00:00
abstract::Temperature elevations were measured in the pulp chamber of full cast crown preparations during impression taking with modelling compound in copper bands. Uncontrolled flaming, controlled flaming, and water heating of the bands produced temperatures of 53, 44, and 41.5 C, respectively. The time involved in restoring t...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:
更新日期:1975-03-01 00:00:00
abstract::The effects of different doses of p-tyramine injected i.v. and i.p. on salivary flow rates and proteins secreted by the submandibular glands of rats were studied with and without various types of autonomic blockers and two enzyme inhibitors. The salivary flow rates and the amounts of protein secreted progressively inc...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345930720051101
更新日期:1993-05-01 00:00:00
abstract::Locally applied bisphosphonates may improve the fixation of metal implants in bone. However, systemic bisphosphonate treatment is associated with a risk of osteonecrosis of the jaw (ONJ). We hypothesized that local delivery of bisphosphonate from the implant surface improves the fixation of dental implants without com...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/0022034512472335
更新日期:2013-03-01 00:00:00
abstract::Composite resin restorations in cavities are generally associated with the development of a contraction gap during the first minutes after insertion. Enamel-Bond-Concise composite restoration were placed on both approximal sides of extracted pre-molars. The cervical walls of all cavities extended beyond the enamel-cem...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345840630051501
更新日期:1984-05-01 00:00:00
abstract::Four disclosant dyes were tested for antimicrobial activity against 27 oral reference strains and five non-oral human pathogens. Erythrosine and fluorescein inhibited most gram-positive and gram-negative organisms associated with dental plaque, and were bactericidal to selected strains. Fast green and brilliant blue d...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345830620071601
更新日期:1983-07-01 00:00:00
abstract::The main purpose of this paper is to review the various methods used for evaluation of fluoride retention in saliva, plaque, and enamel following application of topical anti-caries treatments such as F dentifrices and F mouthwashes. Such methods monitor delivery of fluoride to the site of action, the mouth, and so can...
journal_title:Journal of dental research
pub_type: 杂志文章,评审
doi:10.1177/002203459207100S29
更新日期:1992-04-01 00:00:00
abstract::Gingival overgrowth tissues have thickened connective tissue stroma, sometimes accompanied by the increased presence of collagen fibers, thickened epithelia, and elongated rete pegs. We have previously shown that expression of CCN2, also known as connective tissue growth factor (CTGF), correlates positively with the d...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/0022034511404703
更新日期:2011-07-01 00:00:00
abstract::Polynomial and catenary equations were fit by least square error methods to the dentitions of seven children with "normal" occlusion. Mean and mean square error were then used to analyze accuracy of curve fits and asymmetries of arches. A lack of congruency for the "lines of occlusion" common to the maxilla and mandib...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345750540060501
更新日期:1975-11-01 00:00:00
abstract::Occlusal alterations may result in changes in the functional performance of masticatory muscles. In this study, we set up an experimental model in rats to examine whether masticatory muscle abnormalities occur after a malocclusion is induced. Rats underwent unilateral amputation of the molar cusps to simulate an occlu...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345990780111101
更新日期:1999-11-01 00:00:00
abstract::Periodontal disease is a bacterial infection that results in inflammatory destruction of tissues that support the teeth, including connective tissue and bone. In this study, we report that transgenic mice that overexpress the 17-kDa form of IL-1alpha in the basal layer of oral mucosal epithelium develop a syndrome tha...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/154405910408301010
更新日期:2004-10-01 00:00:00
abstract::In the current theme of dental pulp regeneration, biological and synthetic scaffolds are becoming a potential therapy for pulp revitalization. The goal is to provide a suitable environment for cellular infiltration, proliferation, and differentiation. The extracellular matrix (ECM) represents a natural scaffold materi...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/0022034518785124
更新日期:2018-12-01 00:00:00
abstract::Artificial white spot lesions have been prepared on bovine enamel surfaces by the controlled addition of lactate buffer containing methylhydroxydiphosphonate ions. The rates of remineralization have been measured using a constant composition method in solutions of calcium phosphate of low supersaturation. Deposition o...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345840630061001
更新日期:1984-06-01 00:00:00
abstract::Early-onset periodontitis (EOP) is considered to have a genetic basis which has not been clearly defined. Genetic polymorphisms of the vitamin D receptor (VDR-B-b) and the immunoglobulin-Fc-gamma receptor IIIb (FcgammaRIIIb-NA1-NA2) are associated with bone metabolism and infectious diseases, respectively. The purpose...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345010800120501
更新日期:2001-12-01 00:00:00
abstract::Mucous membrane pemphigoid or cicatricial pemphigoid is a mucocutaneous blistering disease characterized by autoantibodies to different molecules in the basement membrane zone. Our objectives were to identify the target antigen recognized by sera from 20 untreated patients with pemphigoid disease limited to the oral c...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345010800080601
更新日期:2001-08-01 00:00:00