Automated immunofluorescent speciation of oral bacteria using flow cytometry.

Abstract:

:Mixtures containing two bacterial species were analyzed using flow cytometric techniques. Light scattering characteristics of Streptococcus mutans and Actinomyces viscosus show unique profiles for pure cultures. However, the light scatter analysis of a mixture containing these two species demonstrates overlapping near the origin. Thus, light scatter analysis was not sufficient to speciate bacteria with different morphologies. Labeling of samples with species-specific immunofluorescent antibodies permitted speciation of mixtures. As the percentage of the bacterium to which the antibody is directed increased in a two-component mixture, fluorescent flow cytometric analysis showed a corresponding increase in the percentage of cells displaying fluorescent labeling. These methods could permit the rapid identification of bacteria from oral sites without culturing.

journal_name

J Dent Res

authors

Barnett JM,Cuchens MA,Buchanan W

doi

10.1177/00220345840630080501

subject

Has Abstract

pub_date

1984-08-01 00:00:00

pages

1040-2

issue

8

eissn

0022-0345

issn

1544-0591

journal_volume

63

pub_type

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