Characterization of dental pulp stem cells of human tooth germs.

Abstract:

:In previous studies, human dental pulp stem cells (hDPSCs) were mainly isolated from adults. In this present study, we characterized hDPSCs isolated from an earlier developmental stage to evaluate the potential usage of these cells for tissue-regenerative therapy. hDPSCs isolated at the crown-completed stage showed a higher proliferation rate than those isolated at a later stage. When the cells from either group were cultured in medium promoting differentiation toward cells of the osteo/odontoblastic lineage, both became alkaline-phosphatase-positive, produced calcified matrix, and were also capable of forming dentin-like matrix on scaffolds in vivo. However, during long-term passage, these cells underwent a change in morphology and lost their differentiation ability. The results of a DNA array experiment showed that the expression of several genes, such as WNT16, was markedly changed with an increasing number of passages, which might have caused the loss of their characteristics as hDPSCs.

journal_name

J Dent Res

authors

Takeda T,Tezuka Y,Horiuchi M,Hosono K,Iida K,Hatakeyama D,Miyaki S,Kunisada T,Shibata T,Tezuka K

doi

10.1177/154405910808700716

subject

Has Abstract

pub_date

2008-07-01 00:00:00

pages

676-81

issue

7

eissn

0022-0345

issn

1544-0591

pii

87/7/676

journal_volume

87

pub_type

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