Mesenchymal stem/progenitor cell isolation from tooth extraction sockets.

Abstract:

:Bone marrow-derived mesenchymal stem/progenitor cells (BMSCs) are commonly used in regeneration therapy. The current primary source of BMSCs is the iliac crest; however, the procedure is associated with various burdens on the patient, including the risk of pain and infection. Hence, the possibility to collect BMSCs from other, more accessible, sources would be an attractive approach. It is well known that stem cells migrate from surrounding tissues and play important roles in wound healing. We thus hypothesized that stem/progenitor cells could be isolated from granulation tissue in the dental socket, and we subsequently collected granulation tissue from dog dental socket 3 d after tooth extraction. After enzyme digestion of the collected tissue, the cells forming colonies constituted the dental socket-derived stem/progenitor cells (dDSCs). Next, dDSCs were compared with dog BMSCs (dBMSCs) for phenotype characterization. A flow cytometric analysis showed that dDSCs were positive for CD44, CD90, and CD271 but negative for CD34 and CD45, similar to dBMSCs. dDSCs also exhibited osteogenic, adipogenic, and chondrogenic differentiation ability, similar to dBMSCs, with a higher capacity for colony formation, proliferation, and motility than dBMSCs. In addition, an in vivo ectopic bone formation assay showed that dDSCs and dBMSCs both induced hard tissue formation, although only dDSCs formed a fibrous tissue-like structure connected to the newly formed bone. Finally, we tested the ability of dDSCs to regenerate periodontal tissue in a one-wall defect model. The defects in the dDSC-transplanted group (β-TCP/PGA/dDSCs) were regenerated with cementum-like and periodontal ligament-like tissues and alveolar bone, whereas only bony tissue was observed in the control group (β-TCP/PGA). In conclusion, we identified and characterized a population of stem/progenitor cells in granulation tissue obtained from the dental socket that exhibited several characteristics similar to those of BMSCs. Dental sockets could therefore be a novel source for isolating stem/progenitor cells from bone.

journal_name

J Dent Res

authors

Nakajima R,Ono M,Hara ES,Oida Y,Shinkawa S,Pham HT,Akiyama K,Sonoyama W,Maekawa K,Kuboki T

doi

10.1177/0022034514549377

subject

Has Abstract

pub_date

2014-11-01 00:00:00

pages

1133-40

issue

11

eissn

0022-0345

issn

1544-0591

pii

0022034514549377

journal_volume

93

pub_type

杂志文章
  • HEMA enhances IgG1 production by human B-cells in vitro.

    abstract:UNLABELLED:We have previously shown that the resin monomer 2-hydroxyethylmethacrylate (HEMA) affects mouse B-lymphocyte activity, leading to increased IgG1 antibody production in vivo. In the present study, we tested, in vitro, the hypothesis that HEMA also affects human B-lymphocyte activity. The in vitro production o...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/0022034510378522

    authors: Andersson J,Dahlgren U

    更新日期:2010-12-01 00:00:00

  • Posteruptive tooth age in children with Down's syndrome and their sibs.

    abstract::The accumulated posteruptive tooth age was calculated for 212 children with Down's syndrome and 124 of their unaffected sibs. Posteruptive tooth age was shown to vary widely with different morphological permanent tooth types at the same chronological age. Girls in both groups had greater accumulated posteruptive tooth...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345750540033001

    authors: Orner G

    更新日期:1975-05-01 00:00:00

  • The hydration of dental cements.

    abstract::A study was made of the hydration of dental cements, water being classified as "non-evaporable" and "evaporable". The ratio of these two types of water was found to vary greatly among different cement types, being lesser in zinc oxide and ionic polymer cements and greater in ion-leachable glass and phosphoric acid cem...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345790580030701

    authors: Wilson AD,Paddon JM,Crisp S

    更新日期:1979-03-01 00:00:00

  • Alteration of conserved alternative splicing in AMELX causes enamel defects.

    abstract::Tooth enamel is the most highly mineralized tissue in vertebrates. Enamel crystal formation and elongation should be well controlled to achieve an exceptional hardness and a compact microstructure. Enamel matrix calcification occurs with several matrix proteins, such as amelogenin, enamelin, and ameloblastin. Among th...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/0022034514547272

    authors: Cho ES,Kim KJ,Lee KE,Lee EJ,Yun CY,Lee MJ,Shin TJ,Hyun HK,Kim YJ,Lee SH,Jung HS,Lee ZH,Kim JW

    更新日期:2014-10-01 00:00:00

  • Root surface caries in the molar teeth of rice rats. II. Quantitation of lesions induced by high sucrose diet.

    abstract::Weanling rice rats were fed either standard laboratory chow or Diet 2000. The animals were sacrificed at 4, 8, 12, and 15 weeks. There was an increasing amount of exposed root surface and root surface caries with age in the animals fed Diet 2000. There was exposed root surface but no caries in the animals fed laborato...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345770560091701

    authors: Doff RS,Rosen S,App G

    更新日期:1977-09-01 00:00:00

  • Effects of potassium ions on action potential conduction in A- and C-fibers of rat spinal nerves.

    abstract::Potassium ions in dentifrices for treating 'hypersensitive' dentin are believed to act directly on intradental nerves by raising extracellular potassium ion concentration ([K+]o) sufficiently to prevent action potential generation by axonal accommodation. However, the [K+]o necessary to block nerve conduction is not p...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345950740020301

    authors: Peacock JM,Orchardson R

    更新日期:1995-02-01 00:00:00

  • The extent of the odontoblast process in human dentin.

    abstract::Impacted human third molar teeth were examined, both with the light and electron microscope, to determine the extent of the odontoblast process. The odontoblast process was found to be limited to the inner third of coronal dentin and inner half of mid-root dentin. ...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/002203457905800412011

    authors: Thomas HF

    更新日期:1979-11-01 00:00:00

  • Functional characteristics of gingival and periodontal ligament fibroblasts.

    abstract::In periodontal surgery, healing after guided tissue regeneration (GTR) may be explained by differences in functional activities of gingival and periodontal ligament fibroblasts (GF and PDLF). Several studies in vitro have supported this hypothesis, but much remains to be defined. In the present work, gingival and peri...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345960750030601

    authors: Giannopoulou C,Cimasoni G

    更新日期:1996-03-01 00:00:00

  • A preliminary scanning electron microscope examination of wear striation direction on primate teeth.

    abstract::Wear striations experimentally produced on initially unworn teeth were examined at high magnification using a scanning electron microscope. Certain characteristics of individual wear striations on these teeth indicate the direction of motion that produced them. Other striations on worn teeth of American Indians and th...

    journal_title:Journal of dental research

    pub_type: 历史文章,杂志文章

    doi:10.1177/00220345790580011401

    authors: Ryan AS

    更新日期:1979-01-01 00:00:00

  • H3-HRP analysis of the nerve supply to primate teeth.

    abstract::Sensory, sympathetic and parasympathetic ganglia located in the head and neck of rhesus monkeys were histologically examined after injection of H3-HRP into the right mandibular premolars and molars. The results showed positive labeling of ganglionic cell bodies located in the ipsilateral trigeminal, superior cervical,...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345800590041601

    authors: Chiego DJ Jr,Cox CF,Avery JK

    更新日期:1980-04-01 00:00:00

  • Quantification of pulpal blood flow in developing teeth of dogs.

    abstract::Pulpal blood flow was determined in 32 dogs ranging in age from 6 weeks to 18 months by the isotope fractionation method incorporating diffusible and nondiffusible tracers. Dogs were placed into selected age groups by the developmental status of the canine teeth. For the teeth sampled, the maxillary first, second, and...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345770560102601

    authors: Path MG,Meyer MW

    更新日期:1977-10-01 00:00:00

  • Host response in experimental periodontal disease.

    abstract::Experiments were performed to determine the role of the immune response in rat periodontal disease. Germ-free rats were fed defined antigen-free liquid diets or a diet containing ovalbumin(OVA) as a prototype antigen. The OVA-fed rats demonstrated increased gingival lymphocytes (mainly T at early times), OVA-sensitize...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345840630031801

    authors: Taubman MA,Yoshie H,Ebersole JL,Smith DJ,Olson CL

    更新日期:1984-03-01 00:00:00

  • Tensile properties of mineralized and demineralized human and bovine dentin.

    abstract::The relative contribution of the matrix of dentin to the physical properties of dentin is unknown but thought to be small. The objective of this study was to test the hypothesis that the demineralized matrix of dentin contributes little to the strength of dentin by measuring and comparing the ultimate tensile strength...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345940730061201

    authors: Sano H,Ciucchi B,Matthews WG,Pashley DH

    更新日期:1994-06-01 00:00:00

  • The resistance and adaptation of selected oral bacteria to mercury and its impact on their growth.

    abstract::Selected strains of oral Streptococcus and Actinomyces have been tested for their ability to grow in the presence of mercury. Strains were tested for growth on a semi-defined medium with low mercury-binding characteristics. Sensitivities were initially measured on agar plates, and subsequently, selected strains were g...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345930720091201

    authors: Lyttle HA,Bowden GH

    更新日期:1993-09-01 00:00:00

  • Relationships between masticatory muscle cross-section and skull shape.

    abstract::Cross-sectional areas of the masseter, temporalis and medial, and lateral pterygoid muscles were determined in 16 subjects by means of computer tomography. In each subject three scans were made, intersecting the thickest part of the muscles at right angles to the fiber direction. The masseter and medial pterygoid musc...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345840630091201

    authors: Weijs WA,Hillen B

    更新日期:1984-09-01 00:00:00

  • Analysis of Asgar-Mahler reaction zone in Dispersalloy amalgam by electron diffraction.

    abstract::Selected area reflection electron diffraction has been used to determine the phases present in the reaction zones surrounding the Ag-Cu eutectic dispersant in Dispersalloy amalgam. The experiments were carried out on two kinds of specimens, (1) Dispersalloy amalgam and (2) Dispersalloy amalgam using 30, 50, 75, and 10...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345770560090101

    authors: Okabe T,Mitchell R,Butts MB,Bosley JR,Fairhurst CW

    更新日期:1977-09-01 00:00:00

  • Hard tissue formation in subcutaneously transplanted rat dental pulp.

    abstract::While dental pulp appears to be able to form mineralized matrices that do not always resemble dentin, the precise characteristics of the hard tissue and the mechanism of its induction remain unknown. Therefore, we evaluated hard tissue induced by transplantation of pulp into subcutaneous tissue. Seven days after trans...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/154405910708600515

    authors: Hosoya A,Nakamura H,Ninomiya T,Hoshi K,Yoshiba K,Yoshiba N,Takahashi M,Okabe T,Sahara N,Yamada H,Kasahara E,Ozawa H

    更新日期:2007-05-01 00:00:00

  • Tp63 in oral development, neoplasia, and autoimmunity.

    abstract::The Tp63 gene encodes for multiple isoforms of the p63 transcription factor, a member of the p53 family of proteins. Much like its more famous sibling, the biological role of p63 is quite complex, with wide-ranging effects on development, differentiation, and cellular lineage choices. The crucial function of p63 is ep...

    journal_title:Journal of dental research

    pub_type: 杂志文章,评审

    doi:10.1177/0022034511411302

    authors: Romano RA,Solomon LW,Sinha S

    更新日期:2012-02-01 00:00:00

  • RhoA-JNK Regulates the E-Cadherin Junctions of Human Gingival Epithelial Cells.

    abstract::The junctional epithelium (JE) is unique with regard to its wide intercellular spaces and sparsely developed intercellular junctions. Thus, knowledge of the molecular mechanisms that regulate the formation of the intercellular junctions of the junctional epithelium may be essential to understand the pathophysiology of...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/0022034515619375

    authors: Lee G,Kim HJ,Kim HM

    更新日期:2016-03-01 00:00:00

  • 17β-estradiol Induces MMP-9 and MMP-13 in TMJ Fibrochondrocytes via Estrogen Receptor α.

    abstract::Temporomandibular joint (TMJ) disorders, including degenerative TMJ disease, occur primarily in women of reproductive age. Previous studies showed elevated estrogen levels in subjects with TMJ disorders relative to controls and the presence of estrogen receptors α and β (ERα and ERβ) in TMJ fibrocartilage. Additionall...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/0022034518767108

    authors: Ahmad N,Chen S,Wang W,Kapila S

    更新日期:2018-08-01 00:00:00

  • Impact of hydrodynamics on oral biofilm strength.

    abstract::Mechanical removal of oral biofilms is ubiquitously accepted as the best way to prevent caries and periodontal diseases. Removal effectiveness strongly depends on biofilm strength. To investigate the influence of hydrodynamics on oral biofilm strength, we grew single- and multi-species biofilms of Streptococcus oralis...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/0022034509344569

    authors: Paramonova E,Kalmykowa OJ,van der Mei HC,Busscher HJ,Sharma PK

    更新日期:2009-10-01 00:00:00

  • Enhancing oral health via sense of coherence: a cluster-randomized trial.

    abstract::Sense of coherence (SOC) has been related to oral health behaviors and oral-health-related quality of life (OHRQoL) in observational studies. This cluster-randomized trial aimed to test the effect of an intervention to enhance SOC on OHRQoL in children. Twelve primary schools were randomly allocated to intervention an...

    journal_title:Journal of dental research

    pub_type: 杂志文章,随机对照试验

    doi:10.1177/0022034512459757

    authors: Nammontri O,Robinson PG,Baker SR

    更新日期:2013-01-01 00:00:00

  • Late elongation of the cranial base.

    abstract::A single logistic curve was fitted to serial data for three cranial base lengths (S-N, Ba-N, and Ba-S) in 33 boys and 26 girls. Elongation during pubescence plus adolescence is greater in boys than girls for each length. The maximum rate of pubescent elongation occurs earliest in Ba-N. There are no consistent sex diff...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345770560071501

    authors: Roche AF,Lewis AB,Wainer H,McCartin R

    更新日期:1977-07-01 00:00:00

  • Autoantibodies to human alpha6 integrin in patients with oral pemphigoid.

    abstract::Mucous membrane pemphigoid or cicatricial pemphigoid is a mucocutaneous blistering disease characterized by autoantibodies to different molecules in the basement membrane zone. Our objectives were to identify the target antigen recognized by sera from 20 untreated patients with pemphigoid disease limited to the oral c...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345010800080601

    authors: Bhol KC,Goss L,Kumari S,Colon JE,Ahmed AR

    更新日期:2001-08-01 00:00:00

  • Model practice as a valid research tool for studying actual private practice.

    abstract::Data from the actual private practices of two dentists were compared with respective data from the participation of the same two dentists in a laboratory model of private practice conducted in the University of North Carolina Dental Research Center. The findings show that a laboratory model can be established which ac...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345750540032501

    authors: Chadwick DG

    更新日期:1975-05-01 00:00:00

  • Initial subgingival colonization of 'pristine' pockets.

    abstract::The treatment of periodontitis/peri-implantitis involves the reduction/eradication of periopathogens. After therapy, beneficial and pathogenic species recolonize the subgingival area. The dynamics of recolonization and especially the role of the supragingival environment in this process are still not well-understood. ...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/154405910508400409

    authors: Quirynen M,Vogels R,Pauwels M,Haffajee AD,Socransky SS,Uzel NG,van Steenberghe D

    更新日期:2005-04-01 00:00:00

  • Fusobacterium nucleatum activates the immune response through retinoic acid-inducible gene I.

    abstract::Retinoic acid-inducible gene I (RIG-I) is a cytosolic pattern recognition receptor involved in the sensing of RNA viruses and the initiation of antiviral responses. Fusobacterium nucleatum, a Gram-negative anaerobic bacterium associated with periodontal disease, is capable of invading cells. We hypothesized that F. nu...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/0022034513516346

    authors: Lee P,Tan KS

    更新日期:2014-02-01 00:00:00

  • The relationship between clinical tooth status and receipt of sealants among child Medicaid recipients.

    abstract::This study investigated the association between caries status and sealant need at a prior survey and subsequent sealant use in a Medicaid program. Clinical data from a 1986-87 statewide epidemiological survey (N = 8026) representative of North Carolina (NC) schoolchildren (grades K-12) were linked with all NC Medicaid...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345970760120901

    authors: Robison VA,Rozier RG,Weintraub JA,Koch GG

    更新日期:1997-12-01 00:00:00

  • PDGF up-regulates CSF-1 gene transcription in ameloblast-like cells.

    abstract::Macrophage colony-stimulating factor (CSF-1) is a key regulatory cytokine for amelogenesis, and ameloblasts synthesize CSF-1. We hypothesized that PDGF stimulates DNA synthesis and regulates CSF-1 in these cells. We determined the effect of PDGF on CSF-1 expression using MEOE-3M ameloblasts as a model. By RT-PCR, MEOE...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/154405910808700105

    authors: Wittrant Y,Bhandari BS,Abboud H,Benson N,Woodruff K,MacDougall M,Abboud-Werner S

    更新日期:2008-01-01 00:00:00

  • Estimation of mesiodistal width of permanent canines and premolars in early mixed dentition.

    abstract::The accuracy for predicting mesiodistal widths of unerupted permanent canines and premolars from x-rays and their estimations based on the already erupted permanent teeth were tested in a group of Israeli children. The observed posteruptive widths related more closely to predicted values obtained from x-ray measuremen...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345770560081301

    authors: Zilberman Y,Koyoumdjisky-Kaye E,Vardimon A

    更新日期:1977-08-01 00:00:00