Abstract:
:Temporomandibular joint (TMJ) disorders, including degenerative TMJ disease, occur primarily in women of reproductive age. Previous studies showed elevated estrogen levels in subjects with TMJ disorders relative to controls and the presence of estrogen receptors α and β (ERα and ERβ) in TMJ fibrocartilage. Additionally, estrogen-induced overexpression of specific matrix metalloproteinases (MMPs), including MMP-9 and MMP-13, in TMJ fibrocartilage is accompanied by loss of extracellular matrices. However, the contribution of ERα and ERβ in estrogen-mediated induction of MMP-9 and MMP-13 and the signaling cascade leading to the upregulation of these MMPs have not been elucidated. Here, we show that specific siRNAs and selective ER antagonists effectively block ERα or ERβ expression in primary mouse TMJ fibrochondrocytes, but that only blockage of ERα suppresses MMP-9 and MMP-13 levels induced by 17β-estradiol (E2). Overexpression of ERα but not ERβ enhances E2-induced MMP-9. Using the same loss-of-function and gain-of-function approaches, we demonstrate that E2 stimulates ERK activation through ERα and that inhibition of ERK phosphorylation reduces E2-induced MMP-9. Furthermore, we reveal that E2 promotes NF-κB and ELK-1 activation through ERα/ERK signaling and that knockdown of either one decreases the respective activity of these signaling mediators and MMP-9 expression induced by E2, indicating that both contribute to E2/ERα/ERK-mediated MMP-9 upregulation. This is supported by findings in which mutated binding sites of either NF-κB or ELK-1 in the MMP-9 promoter lead to a significant reduction of E2-stimulated promoter activity. Our findings provide novel molecular mechanisms for the understanding of E2-mediated upregulation of MMPs, having implications to pathophysiologic TMJ cartilage matrix turnover that may yield therapeutic intervention targets for TMJ disorders.
journal_name
J Dent Resjournal_title
Journal of dental researchauthors
Ahmad N,Chen S,Wang W,Kapila Sdoi
10.1177/0022034518767108subject
Has Abstractpub_date
2018-08-01 00:00:00pages
1023-1030issue
9eissn
0022-0345issn
1544-0591journal_volume
97pub_type
杂志文章abstract::Dissolution of mercury from the Ag-Hg matrix phase of dental amalgam is distilled water and synthetic saliva, and the mercury evaporation from the solutions, were studied in vitro. The main objectives of the study were to evaluate the factors that affect the results of the mercury release tests, and to consider the po...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345900690050901
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abstract::A/Jax mice were injected with 50 muCi of 3H cortisol and 10 mg of cortisone acetate on day 12 of gestation. At various intervals after injection, the maternal liver, placenta, yolk sac, and the fetal jaws were removed and analyzed by thin-layer chromatography in conjunction with oxidative combustion and liquid scintil...
journal_title:Journal of dental research
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abstract::Epstein-Barr virus (EBV), in addition to its transforming properties, contributes to the pathogenesis of several inflammatory diseases. Here, we investigated its involvement in oral lichen planus (OLP), a common autoimmune-like disease of unknown etiopathogenesis that can display a malignant potential. EBV-infected ce...
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abstract::Periodontal data typically consist of observations made at multiple sites within each patient. Observations within a patient tend to be positively correlated; hence, standard statistical techniques that assume independence are invalid. Regression techniques for correlated data have been proposed; communicating results...
journal_title:Journal of dental research
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abstract::Previous studies have demonstrated that antibodies against cholinoreceptors of exocrine glands correlate with dry mouth in persons with primary Sjögren syndrome (pSS). The aim of the present investigation was to establish if serum IgG antibodies (pSS IgG) were able to interact with cholinoreceptors in rat submandibula...
journal_title:Journal of dental research
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更新日期:2007-09-01 00:00:00
abstract::4-META and new phosphate-methacrylate resins adhere strongly to dental alloys. However, for strengthening the water durability of the adhesive interface, the oxidation of the alloy surface is indispensable. A new oxidation method using ion-sputtering was developed, and the effectiveness of this surface treatment on tw...
journal_title:Journal of dental research
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doi:10.1177/00220345880670110301
更新日期:1988-11-01 00:00:00
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journal_title:Journal of dental research
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doi:10.1177/154405910208101010
更新日期:2002-10-01 00:00:00
abstract::Porphyromonas gingivalis dihydroceramides are found in extracts of calculus-contaminated root surfaces, diseased gingival tissue, and atherosclerotic plaques. These ceramides have been shown to promote inflammatory secretory responses in gingival fibroblasts. Little is known about their effects on the vascular system....
journal_title:Journal of dental research
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journal_title:Journal of dental research
pub_type: 临床试验,杂志文章
doi:10.1177/002203457705600301011
更新日期:1977-10-01 00:00:00
abstract::Extracellular vesicles (EVs), several tens to hundreds of nanometers in size, are vesicles secreted by cells for intercellular communication. EVs released from mesenchymal stem cells (MSC-EVs) have the potential to treat multiple diseases. This study aimed to determine the effects of MSC-EVs on bisphosphonate-related ...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/0022034520906793
更新日期:2020-05-01 00:00:00
abstract::The purpose of this study was to investigate the mechanism of surface layer formation in artificial white spots. Two nondestructive nuclear micro-analysis techniques were employed to analyze windows of human dental enamel at progressive stages of an artificial lesion. Fluoride concentrations were measured to a depth o...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345830620091001
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abstract::The creatinine-adjusted urinary concentration of mercury in 73 schoolchildren with a mean age of 12 years was determined. In addition, the number of amalgam restorations and their size, prevalence of allergy, and days absent from school due to illness were recorded for each individual. A significant positive correlati...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345870660061701
更新日期:1987-06-01 00:00:00
abstract::Chemical bonding between dental porcelains and alloys results from interdiffusion of porcelain and metal ions. An interfacial diffusion zone is created which, most likely, has properties different from those of bulk materials. The changed interface might affect experimental measurements of thermo-mechanical strain. To...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345830620051701
更新日期:1983-05-01 00:00:00
abstract::Growing evidence suggests that individual genetic susceptibility may influence the host's response to infections. The aim of this project was to study whether gene polymorphisms of inflammatory markers are associated with the presence of viable periodontopathogenic bacteria. We extracted genomic DNA from 45 young adul...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/154405910708600505
更新日期:2007-05-01 00:00:00
abstract::Current challenges in adhesive dentistry include over-hydrophilic bonding formulations, which facilitate water percolation through the hybrid layer and result in unreliable bonded interfaces. This study introduces nanogel-modified adhesives as a way to control the material's hydrophobic character without changing the ...
journal_title:Journal of dental research
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doi:10.1177/0022034511426573
更新日期:2012-02-01 00:00:00
abstract::As the use of adhesive restorative materials has increased during the last several years, interest in adhesive materials that release fluoride has also grown. The purpose of this study was to measure fluoride release from several adhesive restorative materials and to evaluate its effect on dentin resistance to deminer...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345990780101001
更新日期:1999-10-01 00:00:00
abstract::After cheek pouch carcinomas were induced in hamsters by the application of dimethylbenzanthracene (DMBA) to the right pouch for 13 weeks, the animals were divided into four groups and observed for seven more weeks. The control group received no further treatment, two experimental groups had incisional biopsies perfor...
journal_title:Journal of dental research
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更新日期:1986-06-01 00:00:00
abstract::Periodontal diseases are initiated by bacteria that accumulate in a biofilm on the tooth surface and affect the adjacent periodontal tissue. Systemic diseases such as diabetes, rheumatoid arthritis (RA), and systemic lupus erythematosus (SLE) increase susceptibility to destructive periodontal diseases. In human studie...
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pub_type: 杂志文章,评审
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journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345780570022701
更新日期:1978-02-01 00:00:00
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journal_title:Journal of dental research
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更新日期:1994-10-01 00:00:00
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journal_title:Journal of dental research
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更新日期:1980-02-01 00:00:00
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journal_title:Journal of dental research
pub_type: 临床试验,杂志文章
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更新日期:1995-09-01 00:00:00
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journal_title:Journal of dental research
pub_type: 杂志文章
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journal_title:Journal of dental research
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journal_title:Journal of dental research
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journal_title:Journal of dental research
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journal_title:Journal of dental research
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更新日期:2017-06-01 00:00:00