Abstract：
:In periodontal surgery, healing after guided tissue regeneration (GTR) may be explained by differences in functional activities of gingival and periodontal ligament fibroblasts (GF and PDLF). Several studies in vitro have supported this hypothesis, but much remains to be defined. In the present work, gingival and periodontal ligament fibroblasts derived from five healthy subjects were isolated and compared in vitro. The morphology of the cells was observed under scanning electron microscopy (SEM). Several extracellular matrix components (ECM) were studied to compare the effects on fibroblast attachment, proliferation, and protein synthesis. Several biochemical markers were examined in both cellular extract (CE) and conditioned medium (CM). We also examined the muscle differentiation markers alpha-smooth muscle actin, desmin, and smooth-muscle myosin. Finally, we studied the effects of epithelial cells on the proliferation and protein synthesis of the two types of fibroblasts. GF and PDLF appeared identical under the SEM. All ECM components enhanced attachment; however, while collagen types I and IV promoted the attachment of GF, gelatin, laminin, and vitronectin promoted that of PDLF. Most ECM components increased the proliferation rate of GF and the biosynthetic activity of PDLF. The biochemical markers were similarly distributed between the two cell types, except for alkaline phosphatase, which was detected only in the CE of PDLF. Both GF and PDLF strongly expressed alpha-smooth-muscle actin and were negative for desmin; only PDLF were positive for smooth-muscle myosin. Epithelial cells increased the proliferation of both GF and PDLF but had no effect on their biosynthetic activity. These in vitro results may better explain the in vivo functional differences between GF and PDLF.
journal_name
J Dent Resjournal_title
Journal of dental researchauthors
Giannopoulou C,Cimasoni Gdoi
10.1177/00220345960750030601subject
Has Abstractpub_date
1996-03-01 00:00:00pages
895-902issue
3eissn
0022-0345issn
1544-0591journal_volume
75pub_type
杂志文章abstract：:Recent advances in molecular biology have facilitated analyses of the oral microbiome ("Who are they?"); however, its functions (e.g., metabolic activities) are poorly understood ("What are they doing?"). This review aims to summarize our current understanding of the metabolism of the oral microbiome. Saccharolytic ba...
journal_title：Journal of dental research
pub_type： 杂志文章,评审
doi：10.1177/0022034515606045
更新日期：2015-12-01 00:00:00
abstract：:The objective of this study was to assess the relative effects of age and oral hygiene on the progression of periodontitis by estimating incidence from age-specific prevalence. This study analyzed data from a representative national sample of 14,690 dentate Americans, aged from 15 to 74, seen in the first National Hea...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345870660010201
更新日期：1987-01-01 00:00:00
abstract：:Resin luting of all-ceramic restorations results in increased performance; however, the strengthening mechanism and the role of the mechanical properties of the resin are not fully understood. The hypothesis tested is that ceramic strength enhancement is dependent on the elastic modulus of the resin. Three-point flexu...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/154405910708600606
更新日期：2007-06-01 00:00:00
abstract：:The number of molars selected for prophylactic removal varies widely among general dental practitioners and oral surgeons. To understand the basis for such variations, we investigated two hypotheses: (1) Individual judgment strategies will differ concerning the use of cues (items of information), and (2) few dentists ...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345000790121101
更新日期：2000-12-01 00:00:00
abstract：:Dental amalgam restorations are a significant source of mercury exposure in the human population, but their potential to cause systemic health effects is highly disputed. We examined effects on the immune system by giving genetically mercury-susceptible Brown Norway (BN) rats and mercury-resistant Lewis (LE) rats silv...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345980770060601
更新日期：1998-06-01 00:00:00
abstract：:Temporomandibular joint (TMJ) disorders, including degenerative TMJ disease, occur primarily in women of reproductive age. Previous studies showed elevated estrogen levels in subjects with TMJ disorders relative to controls and the presence of estrogen receptors α and β (ERα and ERβ) in TMJ fibrocartilage. Additionall...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/0022034518767108
更新日期：2018-08-01 00:00:00
abstract：:Stress-induced changes imparted in a 'dentin-bonded-crown' material during sintering, annealing, pre-cementation surface modification, and resin coating have been visualized by profilometry. The hypothesis tested was that operative techniques modify the stressing pattern throughout the material thickness. We polished ...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/0022034509354049
更新日期：2010-01-01 00:00:00
abstract：:Oxygen inhibits polymerization of resin-based materials. We hypothesized that compromised bonding to bleached enamel can be reversed with sodium ascorbate, an anti-oxidant. Sandblasted human enamel specimens were treated with distilled water (control) and 10% carbamide peroxide gel with or without further treatment wi...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/154405910208100709
更新日期：2002-07-01 00:00:00
abstract：:Enamel abraders used in two-body wear tests suffer the disadvantage that standardization is difficult or impossible to obtain. The aim of this study was to test whether steatite (a ceramic material with a Vickers Hardness similar to that of enamel) could be used as a satisfactory human enamel analogue. The friction an...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345940730091001
更新日期：1994-09-01 00:00:00
abstract：:The aim of this study was to test the hypothesis that the chewing of xylitol- or xylitol/sorbitol-containing chewing gum reduces plaque formation and the acidogenic potential of dental plaque. Thirty healthy volunteers aged from 19 to 28 yrs were randomly allocated to one of three test groups, chewing either xylitol-,...
journal_title：Journal of dental research
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1177/00220345980770071001
更新日期：1998-07-01 00:00:00
abstract：:The effect of combined administration of chlorhexidine and fluoride in the drinking water of hamsters on plaque and caries prevention was studied. Plaque indices of the hamsters receiving chlorhexidine either alone or simultaneously with fluoride were significantly lower than those of the control and fluoride only tre...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345770560111401
更新日期：1977-11-01 00:00:00
abstract：:The tooth resistance measuring instrument was compared to the traditional method of the explorer examination for the detection of occlusal carious lesions. The findings of the instrument were more sensitive as compared to the traditional method when verified by a histological examination. ...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345780570020801
更新日期：1978-02-01 00:00:00
abstract：:The hypothesis that periodontal disease is associated with pre-term low birthweight was tested in a prospective follow-up study of rural prima-gravida women (N = 227) who were free of tobacco, alcohol, and drug use. Women with 3rd trimester mean probing pocket depths, plaque, and bleeding scores that were greater than...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/154405910508400313
更新日期：2005-03-01 00:00:00
abstract：:A numerical model of the mandible, its articulating surfaces, and the forces exerted by the primary masticatory muscles has been developed for the purpose of investigating loading of the temporomandibular joint. Evidence is presented which shows that the temporomandibular joint. Evidence is presented which shows that ...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345860650080201
更新日期：1986-08-01 00:00:00
abstract：:Although the contribution of the oral microbiota to oral malodor is well-documented, the potential role of Gram-positive micro-organisms is unclear. In the current study, we tested the hypothesis that Gram-positive micro-organisms contribute to malodor production by deglycosylating oral glycoproteins, rendering them s...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/154405910608501007
更新日期：2006-10-01 00:00:00
abstract：:Periodontitis is a chronic inflammatory disease affecting oral tissues. The continuous, high production of cytokines by host cells triggered by periodontopathogens is thought to be responsible for the destruction of tooth-supporting tissues. Macrophages play a critical role in this host inflammatory response to period...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/154405910608500306
更新日期：2006-03-01 00:00:00
abstract：:In this study, the effects of eugenol on human polymorphonuclear (PMN) cell migration and chemiluminescence were examined in vitro. Utilizing zymosan-activated serum or crude Bacteroides sonicate fractions as chemotractants, we found that eugenol inhibits PMN migration at 6.6 X 10(-2) to 6.6 X 10(-5) mol/L (P less tha...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345870660031401
更新日期：1987-03-01 00:00:00
abstract：:Dimensional alterations of the facial bone wall following tooth extractions in the esthetic zone have a profound effect on treatment outcomes. This prospective study in 39 patients is the first to investigate three-dimensional (3D) alterations of facial bone in the esthetic zone during the initial 8 wks following flap...
journal_title：Journal of dental research
pub_type： 临床试验,杂志文章
doi：10.1177/0022034513506713
更新日期：2013-12-01 00:00:00
abstract：:Better understanding of dental caries and other oral conditions has guided new strategies to prevent disease and manage its consequences at individual and public health levels. This article discusses advances in prevention and minimal intervention dentistry over the last century by focusing on some milestones within s...
journal_title：Journal of dental research
pub_type： 历史文章,杂志文章
doi：10.1177/0022034519837252
更新日期：2019-06-01 00:00:00
abstract：:The efficacy of a new remineralization system was determined in vivo by maintaining a low concentration of approximately 1 ppm fluoride for 48 hrs against a demineralized human tooth. Human subjects were selected who wore removable partial dentures containing two or more of the demineralized teeth with film system. Th...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345800590030501
更新日期：1980-03-01 00:00:00
abstract：:Temperature elevations were measured in the pulp chamber of full cast crown preparations during impression taking with modelling compound in copper bands. Uncontrolled flaming, controlled flaming, and water heating of the bands produced temperatures of 53, 44, and 41.5 C, respectively. The time involved in restoring t...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：
更新日期：1975-03-01 00:00:00
abstract：:We explored the potential to diagnose Zika virus (ZIKV) infection by analyzing peptides in saliva during a convalescent phase of infection, long after resolution of acute disease. A 25-y-old woman clinically diagnosed with Zika fever in the first trimester was enrolled with her dizygotic twins for a 3-mo postnatal sam...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/0022034517723325
更新日期：2017-09-01 00:00:00
abstract：:The fluid in and gingival tissue lining periodontal pockets were collected from male patients undergoing periodontal therapy. The collagenolytic activity of the crevicular fluid, and the ability of the gingiva in culture to degrade (a) an exogenous collagen substrate, and (b) endogenous collagen newly synthesized and ...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345790580111601
更新日期：1979-11-01 00:00:00
abstract：:In this study, we first investigated the expressions of Jagged1, Notch2, the receptor activator of nuclear factor-kappa B ligand (RANKL), and interleukin (IL)-6 in areas of root resorption during experimental tooth movement in rats in vivo. We then assessed the effects of compression force (CF) with or without GSI (an...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/0022034514555364
更新日期：2015-01-01 00:00:00
abstract：:The biogenetic formation of mineral crystals, one aspect of biomineralization, is a multistep process of apatite formation throughout the growth of dentin tissue. An important step is the transformation of the non-mineralized predentin matrix to mineralizing dentin matrix and its biological control. In this study, the...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345980770081401
更新日期：1998-08-01 00:00:00
abstract：:The discovery of the phenomenon "osseointegration," or functional ankylosis, has led to the development of oral implants with high clinical performance. Consequently, the placement of titanium implants has changed the paradigms of restorative dentistry. Implants are used to prevent placing reconstructions anchored on ...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/0022034519853574
更新日期：2019-11-01 00:00:00
abstract：:Overgrowth of oral yeast is a common problem among the elderly. Probiotic bacteria are known to inhibit the growth of pathogenic microbes. We tested the hypothesis that cheese containing probiotic bacteria can reduce the prevalence of oral Candida. During this 16-week, randomized, double-blind, placebo-controlled stud...
journal_title：Journal of dental research
pub_type： 杂志文章,随机对照试验
doi：10.1177/154405910708600204
更新日期：2007-02-01 00:00:00
abstract：:The aim of this study was to investigate the diffusion dynamics of 25% hydrogen peroxide (H2O2) through enamel-dentin layers and to correlate it with dentin's structural alterations. Micro-Raman Spectroscopy (MRS) and Fourier Transform Infrared Photoacoustic Spectroscopy (FTIR-PAS) were used to measure the spectra of ...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/0022034513488893
更新日期：2013-07-01 00:00:00
abstract：:A new type of hydraulic cement has been developed. This cement consists of a mixture of cement powder and setting agent powder and therefore has only to be kneaded with a little amount of water when being used. ...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345790580050601
更新日期：1979-05-01 00:00:00
abstract：:The universally accepted concept of delay-loaded dental implants has recently been challenged. This study hypothesizes that early loading (decreased implant healing time) leads to increased bone formation and decreased crestal bone loss. We used 17 minipigs to study implants under a controlled load, with non-loaded im...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/154405910308200803
更新日期：2003-08-01 00:00:00
