Abstract:
:Tooth enamel is the most highly mineralized tissue in vertebrates. Enamel crystal formation and elongation should be well controlled to achieve an exceptional hardness and a compact microstructure. Enamel matrix calcification occurs with several matrix proteins, such as amelogenin, enamelin, and ameloblastin. Among them, amelogenin is the most abundant enamel matrix protein, and multiple isoforms resulting from extensive but well-conserved alternative splicing and postsecretional processing have been identified. In this report, we recruited a family with a unique enamel defect and identified a silent mutation in exon 4 of the AMELX gene. We show that the mutation caused the inclusion of exon 4, which is almost always skipped, in the mRNA transcript. We further show, by generating and characterizing a transgenic animal model, that the alteration of the ratio and quantity of the developmentally conserved alternative splicing repertoire of AMELX caused defects in enamel matrix mineralization.
journal_name
J Dent Resjournal_title
Journal of dental researchauthors
Cho ES,Kim KJ,Lee KE,Lee EJ,Yun CY,Lee MJ,Shin TJ,Hyun HK,Kim YJ,Lee SH,Jung HS,Lee ZH,Kim JWdoi
10.1177/0022034514547272subject
Has Abstractpub_date
2014-10-01 00:00:00pages
980-7issue
10eissn
0022-0345issn
1544-0591pii
0022034514547272journal_volume
93pub_type
杂志文章abstract::Two copper-free amalgams expanded excessively after storage in air at 60 degrees C for about five yr. One expanded significantly at 37 degrees C and slightly at 23 degrees C. High silver content, absence of copper, and presence of small amounts of zinc, or all three, seemed to promote very high expansions at 60 degree...
journal_title:Journal of dental research
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journal_title:Journal of dental research
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journal_title:Journal of dental research
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abstract::Fibroblast growth factor receptor 2 (FGFR2) in craniofacial bones mediates osteoprogenitor proliferation, differentiation, and apoptosis. The distortion of proper craniofacial bone growth may cause class II and class III skeletal malocclusion and result in compromised function and aesthetics. Here, we investigated the...
journal_title:Journal of dental research
pub_type: 杂志文章
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abstract::Amelogenin gene organization varies from 6 exons (1,2,3,5,6,7) in amphibians and sauropsids to 10 in rodents. The additional exons are exons 4, 8, 9, and "4b", the latter being as yet unidentified in AMELX transcripts. To learn more about the evolutionary origin of these exons, we used an in silico approach to find th...
journal_title:Journal of dental research
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abstract::Breastfeeding is a powerful health-promoting behavior. A 2016 Lancet global collaboration to review the health implications of breastfeeding was among the first to consider oral health outcomes. While a role was suggested for breastfeeding in preventing malocclusion, caries was the only included disease condition unfa...
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journal_title:Journal of dental research
pub_type: 杂志文章
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journal_title:Journal of dental research
pub_type: 杂志文章
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journal_title:Journal of dental research
pub_type: 杂志文章
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abstract::Strength and durability of the dentin-acrylic resin joint cemented with 2-cyanoacrylate esters were studied. Maximum adhesion is obtained with isobutyl 2-cyanoacrylate after 1% acid pretreatment of the dentin. Hydrolytic stability is somewhat improved by addition of polymer to the adhesive or coating around the joint....
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345790580090901
更新日期:1979-09-01 00:00:00
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journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/154405910608501017
更新日期:2006-10-01 00:00:00
abstract::In this study, we first investigated the expressions of Jagged1, Notch2, the receptor activator of nuclear factor-kappa B ligand (RANKL), and interleukin (IL)-6 in areas of root resorption during experimental tooth movement in rats in vivo. We then assessed the effects of compression force (CF) with or without GSI (an...
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pub_type: 杂志文章
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abstract::Heads of Swiss-Webster mouse fetuses of four ages spanning days 12-13 of gestation, were partially dissected by removing the brain (B), tongue (T) and mandible (M) alone or in combination (BT, BM, BTM). Preparations were suspended in a gassed, circulating culture system such that palatal closure must take place agains...
journal_title:Journal of dental research
pub_type: 杂志文章
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journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345780570012001
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abstract::In a three-year study of 145 adolescent males in a boarding school in Wisconsin, there were no statistically significant differences in dental caries experience between a group consuming phosphates added to pre-sweetened and non-presweetened ready-to-eat cereals, and the control group, or between cereal eaters and non...
journal_title:Journal of dental research
pub_type: 临床试验,杂志文章
doi:10.1177/00220345790580090201
更新日期:1979-09-01 00:00:00
abstract::Proteolytic hydrolysis of casein and porcine or bovine glycoproteins by S mutans was demonstrated in the present study. Caseinolytic activity was found in both the soluble contents of the cells and the cellular debris after rupture of the cells. However, caseinolytic activity could not be demonstrated after growth in ...
journal_title:Journal of dental research
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journal_title:Journal of dental research
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journal_title:Journal of dental research
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pub_type: 杂志文章,随机对照试验
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更新日期:2008-05-01 00:00:00
abstract::Smoking has been established as an important risk factor for periodontal disease and tooth loss. The purpose of this study was a prospective evaluation of the effects of smoking on dental care utilization and its costs, based on data from 5712 males aged 20-59 yrs. Age, dental health behavior, and history of diabetes ...
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pub_type: 杂志文章
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journal_title:Journal of dental research
pub_type: 杂志文章,多中心研究,随机对照试验
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更新日期:2018-02-01 00:00:00
abstract::The Kubelka-Munk (K-M) theory provides a reflectance model for translucent materials placed on backings of different colors. We hypothesize that Kubelka-Munk (K-M) theoretical diffuse reflectance spectra of dentin and enamel are in good agreement with observed diffuse reflectance. The aim of this study was to measure ...
journal_title:Journal of dental research
pub_type: 杂志文章
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abstract::The discovery of the phenomenon "osseointegration," or functional ankylosis, has led to the development of oral implants with high clinical performance. Consequently, the placement of titanium implants has changed the paradigms of restorative dentistry. Implants are used to prevent placing reconstructions anchored on ...
journal_title:Journal of dental research
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abstract::The effects of oral rinses on enamel fluoride uptake, caries, and plaque pH in rats were investigated by use of (1) an acidic calcium phosphate solution (CPS) saturated with dicalcium phosphate dihydrate (DCPD) and (2) a 1% fluoride solution. Osborne-Mendel rats, 19 days of age, were randomly assigned to one of four m...
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abstract::Information on the time-dependent release of fluoride from filled resins containing fluoride particles as a function of particle content and solution pH is limited. This study characterized the fluoride ion release from filled resins containing CaF2 particles as a function of filler content and pH. Urethane dimethacry...
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pub_type: 杂志文章
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