Hard tissue formation in subcutaneously transplanted rat dental pulp.

Abstract:

:While dental pulp appears to be able to form mineralized matrices that do not always resemble dentin, the precise characteristics of the hard tissue and the mechanism of its induction remain unknown. Therefore, we evaluated hard tissue induced by transplantation of pulp into subcutaneous tissue. Seven days after transplantation, initial hard tissue was formed at the inner periphery of the pulp. After 14 days, this hard tissue expanded inwardly. Mineralized matrix was immunopositive for osteocalcin, osteopontin, and bone sialoprotein, but negative for dentin sialoprotein. Transplantation of GFP-labeled pulp into wild-type rats showed these formative cells to have been derived from the transplant. TEM observation revealed apatite crystals within necrotic cells and matrix vesicles at the initial stage of calcification. These results indicate that pulp cells possess the ability to form a bone- or cementum-like matrix. Calcification of the matrix may occur in necrotic cells and matrix vesicles, followed by collagenous calcification.

journal_name

J Dent Res

authors

Hosoya A,Nakamura H,Ninomiya T,Hoshi K,Yoshiba K,Yoshiba N,Takahashi M,Okabe T,Sahara N,Yamada H,Kasahara E,Ozawa H

doi

10.1177/154405910708600515

subject

Has Abstract

pub_date

2007-05-01 00:00:00

pages

469-74

issue

5

eissn

0022-0345

issn

1544-0591

pii

86/5/469

journal_volume

86

pub_type

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