Mineralized tissue formation by BMP2-transfected pulp stem cells.

Abstract:

:Previously, in vitro differentiation of odontoblasts was shown for dental pulp stem cells (DPSCs) transfected with bone morphogenetic protein-2 (Bmp2). For this study, we hypothesized that such cells also show potential for mineralized tissue formation in vivo. DPSCs were transfected with Bmp2 and seeded onto a ceramic scaffold. These complexes were cultured in medium without dexamethasone, and thereafter placed subcutaneously in nude mice for 1, 4, and 12 weeks. Samples were evaluated by histology and real-time PCR for osteocalcin, bone sialoprotein, dentin sialophosphoprotein, and dentin matrix protein 1. Results indicated that only the transfected DPSCs showed obvious mineralized tissue generation, and 12 weeks of implantation gave the highest percentage of mineralized tissue formation (33 +/- 7.3% of implant pore area). Real-time PCR confirmed these results. In conclusion, Bmp2-transfected DPSCs effectively show mineralized tissue formation upon ectopic implantation.

journal_name

J Dent Res

authors

Yang X,van der Kraan PM,Bian Z,Fan M,Walboomers XF,Jansen JA

doi

10.1177/0022034509346258

subject

Has Abstract

pub_date

2009-11-01 00:00:00

pages

1020-5

issue

11

eissn

0022-0345

issn

1544-0591

pii

88/11/1020

journal_volume

88

pub_type

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