Abstract:
:Previously, in vitro differentiation of odontoblasts was shown for dental pulp stem cells (DPSCs) transfected with bone morphogenetic protein-2 (Bmp2). For this study, we hypothesized that such cells also show potential for mineralized tissue formation in vivo. DPSCs were transfected with Bmp2 and seeded onto a ceramic scaffold. These complexes were cultured in medium without dexamethasone, and thereafter placed subcutaneously in nude mice for 1, 4, and 12 weeks. Samples were evaluated by histology and real-time PCR for osteocalcin, bone sialoprotein, dentin sialophosphoprotein, and dentin matrix protein 1. Results indicated that only the transfected DPSCs showed obvious mineralized tissue generation, and 12 weeks of implantation gave the highest percentage of mineralized tissue formation (33 +/- 7.3% of implant pore area). Real-time PCR confirmed these results. In conclusion, Bmp2-transfected DPSCs effectively show mineralized tissue formation upon ectopic implantation.
journal_name
J Dent Resjournal_title
Journal of dental researchauthors
Yang X,van der Kraan PM,Bian Z,Fan M,Walboomers XF,Jansen JAdoi
10.1177/0022034509346258subject
Has Abstractpub_date
2009-11-01 00:00:00pages
1020-5issue
11eissn
0022-0345issn
1544-0591pii
88/11/1020journal_volume
88pub_type
杂志文章abstract::Mucous membrane pemphigoid or cicatricial pemphigoid is a mucocutaneous blistering disease characterized by autoantibodies to different molecules in the basement membrane zone. Our objectives were to identify the target antigen recognized by sera from 20 untreated patients with pemphigoid disease limited to the oral c...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345010800080601
更新日期:2001-08-01 00:00:00
abstract::Current knowledge regarding the sensitivity of the teeth to forces is based on psychophysical experiments that measured touch detection thresholds under static jaw conditions. It is not known whether jaw movements alter the perception of forces applied to the teeth, but, based on limb movement studies, it is hypothesi...
journal_title:Journal of dental research
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doi:10.1177/0022034510363101
更新日期:2010-04-01 00:00:00
abstract::Little is known about the oral health of the frail and home-living elderly. The effects of dentogenic infections on the general condition of the elderly are also unknown. We therefore set out to investigate 191 elderly patients referred to an acute geriatric hospital due to sudden worsening of their general health. Th...
journal_title:Journal of dental research
pub_type: 杂志文章
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journal_title:Journal of dental research
pub_type: 临床试验,杂志文章
doi:10.1177/00220345950740120701
更新日期:1995-12-01 00:00:00
abstract::Disks of dentin taken from extracted human teeth demonstrated the optical property of the magnification/reduction effect. When images were transmitted from a pulpal-to-occlusal direction, the images were magnified. When the disks were inverted, the images were reduced in size. The images were slightly distorted but cl...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345760550041601
更新日期:1976-07-01 00:00:00
abstract::Deciduous teeth were collected from children who received professional prophylaxis with an acidulated phosphate-fluoride (APF) or placebo paste and were analyzed to determine fluoride uptake. Enamel fluoride level was increased by the APF paste approximately 300 ppm at a depth of 2.5 micrometers during the first month...
journal_title:Journal of dental research
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doi:10.1177/00220345760550064901
更新日期:1976-11-01 00:00:00
abstract::The amount of free water in 50 samples of air-dried enamel from permanent and deciduous teeth was measured by the Karl Fischer method. Samples included both contemporary and burial specimens. The mean values obtained showed that free water constituted about 1% of enamel mass. However, the range of individual values va...
journal_title:Journal of dental research
pub_type: 历史文章,杂志文章
doi:10.1177/00220345880670051701
更新日期:1988-05-01 00:00:00
abstract::The treatment of periodontitis/peri-implantitis involves the reduction/eradication of periopathogens. After therapy, beneficial and pathogenic species recolonize the subgingival area. The dynamics of recolonization and especially the role of the supragingival environment in this process are still not well-understood. ...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/154405910508400409
更新日期:2005-04-01 00:00:00
abstract::Fibroblast growth factor (FGF) signaling has been implicated in the regulation of osteogenesis in both intramembranous and endochondral ossifications. In the developing palate, the anterior bony palate forms by direct differentiation of cranial neural crest (CNC)-derived mesenchymal cells, but the signals that regulat...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/0022034517750141
更新日期:2018-05-01 00:00:00
abstract::Eugenol, in the 100 percent concentration in commercial use, was applied to a circumscribed area, 3 mm in diameter, of rat labial mucosa for 1 minute. Reaction periods of 15 minutes, 1, 2, 4 and 6 hours were then permitted. Using routine histological procedures for processing the experimental tissues, it was observed ...
journal_title:Journal of dental research
pub_type: 杂志文章
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更新日期:1978-11-01 00:00:00
abstract::A full understanding of the key regulators controlling periodontal development and homeostasis is necessary for the design of improved periodontal regenerative therapies. Small leucine-rich proteoglycans (SLRPs) are extracellular matrix molecules suggested to regulate collagen organization and cell signaling. Mice wit...
journal_title:Journal of dental research
pub_type: 杂志文章
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更新日期:2014-08-01 00:00:00
abstract::The universally accepted concept of delay-loaded dental implants has recently been challenged. This study hypothesizes that early loading (decreased implant healing time) leads to increased bone formation and decreased crestal bone loss. We used 17 minipigs to study implants under a controlled load, with non-loaded im...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/154405910308200803
更新日期:2003-08-01 00:00:00
abstract::A gravity sintering fabrication technique has been developed for producing Co-Cr-Mo alloy dental implants having a porous coating on the root portion. This process allows for control of pore structure enabling ingrowth of either bone or fibrous tissue. The microstructure and mechanical properties of these materials ha...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345770560050501
更新日期:1977-05-01 00:00:00
abstract::Acculturation is a complex phenomenon that can serve as a proxy for cultural norms and behaviors affecting care-seeking, prevention behaviors, and, ultimately, health outcomes. The purpose of this study was to assess the effect of acculturation on the oral health of Haitian immigrants in New York City. We hypothesized...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/154405910408300219
更新日期:2004-02-01 00:00:00
abstract::In this study, we investigated whether selective activation of nociceptive primary afferent fibers by capsaicin would induce modulations on tooth-pulp-evoked sensory or inhibitory masseter reflex responses in healthy human subjects. The contribution of central N-methyl-D-aspartate (NMDA) receptor mechanisms in capsaic...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345970760090801
更新日期:1997-09-01 00:00:00
abstract::The effects of Escherichia coli endotoxin upon mouse L929 cell proliferation, DNA synthesis, protein synthesis, and proline incorporation were determined. It was found that a level of endotoxin which inhibited cell proliferation prompted a similar inhibition of DNA synthesis and overall cell protein synthesis. In cont...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345790580061301
更新日期:1979-06-01 00:00:00
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journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345950740020301
更新日期:1995-02-01 00:00:00
abstract::Khat-chewing has been associated with oral lesions including oral cancer, but the mechanisms leading to their development are not known. We hypothesized that khat interferes with the physiological processes of the oral mucosa, such as cell proliferation and differentiation, and aimed at investigating the effects of kh...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/0022034509354980
更新日期:2010-03-01 00:00:00
abstract::Enamel abraders used in two-body wear tests suffer the disadvantage that standardization is difficult or impossible to obtain. The aim of this study was to test whether steatite (a ceramic material with a Vickers Hardness similar to that of enamel) could be used as a satisfactory human enamel analogue. The friction an...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345940730091001
更新日期:1994-09-01 00:00:00
abstract:UNLABELLED:We have previously shown that the resin monomer 2-hydroxyethylmethacrylate (HEMA) affects mouse B-lymphocyte activity, leading to increased IgG1 antibody production in vivo. In the present study, we tested, in vitro, the hypothesis that HEMA also affects human B-lymphocyte activity. The in vitro production o...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/0022034510378522
更新日期:2010-12-01 00:00:00
abstract::Since edentate subjects have a reduced masticatory function, it can be expected that the morphology of the cancellous bone of their mandibular condyles has changed according to the altered mechanical environment. In the present study, the morphology of cylindrical cancellous bone specimens of the mandibular condyles o...
journal_title:Journal of dental research
pub_type: 杂志文章
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更新日期:2004-03-01 00:00:00
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pub_type: 杂志文章,评审
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abstract::We have previously reported the clinical and radiographic findings of a trial in dogs of a new dental implant system after a functional period of eight months. The present report consists of the corresponding qualitative and quantitative histological data. The implant system was fabricated from Ti-6A1-4V and employed ...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345880670090801
更新日期:1988-09-01 00:00:00
abstract::Enamel crystals in the demineralized zones in early caries lesions of human teeth were observed by high-resolution electron microscopy. The enamel crystals frequently exhibited perforations in their centers and defects of various sizes on their lateral surfaces. There were a number of small electron-lucent spots, sugg...
journal_title:Journal of dental research
pub_type: 杂志文章
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更新日期:1987-11-01 00:00:00
abstract:UNLABELLED:The aim of this observational study was to investigate the features of the chewing activity and the variability of the human chewing pace, as assessed in the natural environment. It was hypothesized that the chewing pace is relatively constant within individuals across different days but is variable across i...
journal_title:Journal of dental research
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更新日期:2011-10-01 00:00:00
abstract::To understand the role of betel quid (BQ) in the pathogenesis of oral submucous fibrosis (OSF) and oral cancer, we used DNA damage, cytotoxicity, and cell proliferation assays to study the pathobiological effects of aqueous extracts of three BQ constituents [betel nut (Areca catechu, BN), inflorescence of Piper betle ...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345940730050501
更新日期:1994-05-01 00:00:00
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journal_title:Journal of dental research
pub_type: 杂志文章
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更新日期:2016-10-01 00:00:00
abstract::In a standard periodontal treatment strategy with consecutive root planings (per quadrant at a one- to two-week interval), re-infection of a disinfected area might occur before completion of the treatment. This study examines, both clinically and microbiologically, whether a full-mouth disinfection within 24 hours sig...
journal_title:Journal of dental research
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1177/00220345950740080501
更新日期:1995-08-01 00:00:00
abstract::1. A method has been developed and described for obtaining accurate thermal diffusivity values for dental cements and other restorative materials. This method employs specimens of a short cylindrical geometry which are readily fabricated from simply-constructed molds. 2. Results obtained for polycarboxylate and glass-...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345810600120801
更新日期:1981-12-01 00:00:00
abstract::Recent functional animal studies have reported that the motor control of masticatory muscle function is bilaterally guided by both hemispheres, which may fundamentally differ from the cortical control of limb muscle function. In this study, we investigated whether unilateral cortical brain infarction induces different...
journal_title:Journal of dental research
pub_type: 杂志文章
doi:10.1177/00220345990780120701
更新日期:1999-12-01 00:00:00