Abstract：
:Retinoic acid-inducible gene I (RIG-I) is a cytosolic pattern recognition receptor involved in the sensing of RNA viruses and the initiation of antiviral responses. Fusobacterium nucleatum, a Gram-negative anaerobic bacterium associated with periodontal disease, is capable of invading cells. We hypothesized that F. nucleatum's ability to invade cells allows the microorganism to activate the immune response through RIG-I. Bacterial invasion was found to be necessary for F. nucleatum-induced nuclear factor kappa B (NF-κB) activation. Following invasion of the human periodontal ligament fibroblast (PDLF), F. nucleatum was located in the cytosol. F. nucleatum infection led to an 80-fold increase in RIG-I expression. Silencing RIG-I in PDLF by siRNA led to a significant decrease of NF-κB activation and expression of proinflammatory genes. Additionally, F. nucleatum was able to secrete nucleic acids, and introduction of F. nucleatum RNA into PDLF led to a RIG-I-dependent activation of NF-κB. Our findings showed RIG-I to be involved in the recognition of F. nucleatum. The function of RIG-I is likely to be broad and not limited to sensing of viruses only. Hence, this receptor may play an important role in detecting invasive forms of oral pathogens and contribute to inflammation in periodontal tissues.
journal_name
J Dent Resjournal_title
Journal of dental researchauthors
Lee P,Tan KSdoi
10.1177/0022034513516346subject
Has Abstractpub_date
2014-02-01 00:00:00pages
162-8issue
2eissn
0022-0345issn
1544-0591pii
0022034513516346journal_volume
93pub_type
杂志文章abstract：:The study of biofilm structure and function mandates the use of model systems for which a host of environmental variables can be rigorously controlled. We describe a model of supragingival plaque containing Actinomyces naeslundii, Veillonella dispar, Fusobacterium nucleatum, Streptococcus sobrinus, and Streptococcus o...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345010800011201
更新日期：2001-01-01 00:00:00
abstract：:Intra-oral wounds, like wounds in children, demonstrate privileged healing when compared with adult wounds at extra-oral sites. This study investigated whether this preferential healing is related to an increased ability of oral mucosal fibroblasts to reorganize extracellular matrix (ECM) when compared with their derm...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345960750060601
更新日期：1996-06-01 00:00:00
abstract：:Dental caries can be described as a dysbiosis of the oral microbial community, in which acidogenic, aciduric, and acid-adapted bacterial species promote a pathogenic environment, leading to demineralization. Alkali generation by oral microbes, specifically via arginine catabolic pathways, is an essential factor in mai...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/0022034517707512
更新日期：2017-07-01 00:00:00
abstract：:Current knowledge regarding the sensitivity of the teeth to forces is based on psychophysical experiments that measured touch detection thresholds under static jaw conditions. It is not known whether jaw movements alter the perception of forces applied to the teeth, but, based on limb movement studies, it is hypothesi...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/0022034510363101
更新日期：2010-04-01 00:00:00
abstract：:Repulsive magnetic appliances can intrude posterior teeth, but create a lateral shift of the mandible and also decrease in force as the mouth opens. To model their optimal use, the 3-D spatial force/displacement (F/D) and moment/displacement (M/D) diagrams of four magnetic repulsive appliances in diverse overlapping a...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345940730010901
更新日期：1994-01-01 00:00:00
abstract：:This randomized controlled trial aimed to evaluate the duration and outcome quality of orthodontic treatment with a customized fixed appliance system versus a noncustomized system. Patients ( n = 180) were randomized and received orthodontic treatment with the Insignia customized orthodontic system or the Damon Q nonc...
journal_title：Journal of dental research
pub_type： 杂志文章,随机对照试验
doi：10.1177/0022034517720913
更新日期：2017-12-01 00:00:00
abstract：:Adenosine has various biological effects on human gingival fibroblasts (HGF) and epithelial cells closely associated with inflammation, such as cytokine production and cell adhesion. However, the mechanism of adenosine formation in periodontal tissues is not yet defined. In this study, we examined the involvement of C...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/154405910308201108
更新日期：2003-11-01 00:00:00
abstract：:Mesenchymal stem cells isolated from different dental tissues have been described to have osteogenic/odontogenic-like differentiation capacity, but little attention has been paid to the biochemical composition of the material that each produces. Here, we used Raman spectroscopy to analyze the mineralized materials pro...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/0022034515599765
更新日期：2015-11-01 00:00:00
abstract：:The flexure moduli of elasticity of solid and multi-stranded orthodontic wires were determined. Results for the solid wires approximated their tensile values, but the differences were statistically significant for the stainless steel, chromium-cobalt-nickel, and nickel-titanium alloys. The flexure moduli of the multi-...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345830620072201
更新日期：1983-07-01 00:00:00
abstract：:Four disclosant dyes were tested for antimicrobial activity against 27 oral reference strains and five non-oral human pathogens. Erythrosine and fluorescein inhibited most gram-positive and gram-negative organisms associated with dental plaque, and were bactericidal to selected strains. Fast green and brilliant blue d...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345830620071601
更新日期：1983-07-01 00:00:00
abstract：:Epstein-Barr virus (EBV), in addition to its transforming properties, contributes to the pathogenesis of several inflammatory diseases. Here, we investigated its involvement in oral lichen planus (OLP), a common autoimmune-like disease of unknown etiopathogenesis that can display a malignant potential. EBV-infected ce...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/0022034518788282
更新日期：2018-12-01 00:00:00
abstract：:Lipopolysaccharide (LPS) from the Gram-negative pathogen Porphyromonas gingivalis (Pg) stimulates cytokine secretion in immune cells, and thereby initiates the inflammation associated with periodontitis. Modulation of pro-inflammatory cytokine activity is a plausible therapeutic target in periodontal disease. Vasoacti...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/154405910508401106
更新日期：2005-11-01 00:00:00
abstract：:The periodontium contains heterogeneous mesenchymal cell populations with various differentiation potentials. The capacity of these cells for tissue formation as well as the origin of their precursors are still not entirely defined. In this study, cells originating from different periodontal tissues were cultured in v...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345950740051201
更新日期：1995-05-01 00:00:00
abstract：:Catecholamines are present in saliva, but their influence on oral epithelium is not understood. Because psychological stress increases salivary catecholamines and impairs oral mucosal wound healing, we sought to determine if epithelial adrenergic signaling could link these two findings. We found that cultured human or...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/0022034510388034
更新日期：2011-02-01 00:00:00
abstract：:In this study, environmental stains were removed from maxillofacial elastomers by solvent extraction. Silastic 44210, an RTV silicone with proven color and physical property stability, was stained with lipstick, disclosing solution, and methylene blue. These stains were then removed by solvent extraction with each of ...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345810600100501
更新日期：1981-10-01 00:00:00
abstract：:Mixed or parotid saliva from caries-active individuals consistently supported better growth of Streptococcus mutans (type c) than that from caries-free individuals. Electrophoretic studies revealed that certain proteins in caries-active salivas were susceptible to microbial attack, but similar proteins in caries-free ...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345790580101501
更新日期：1979-10-01 00:00:00
abstract：:Short-chain carboxylic acids (e.g., lactic acid, propionic acid, butyric acid) are metabolic by-products of bacterial metabolism which can accumulate in the gingival crevice. It is of no small consequence, therefore, that 1- to 5-mM concentrations of these acids exhibit significant biological activity, including the a...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345970760010801
更新日期：1997-01-01 00:00:00
abstract：:To date, the precise etiology of molar-incisor hypomineralization (MIH) is uncertain. Vitamin D plays a key role in hard tissue formation. Therefore, this study aimed to analyze the relationship between serum 25-hydroxy-vitamin D (25(OH)D) status and dental health data obtained from 1,048 children in a 10-year follow-...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/0022034514561657
更新日期：2015-02-01 00:00:00
abstract：:The temporal and spatial patterns in which teeth are initiated in the growing jaws of embryos are constant for a species but different for different species. The sources of the patterns have been explained in two ways. First, they are the outcome of reactions between molecules created at stationary targets and those w...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345980770090901
更新日期：1998-09-01 00:00:00
abstract：:Stable fluoride-resistant mutants of Streptococcus mutans GS-5 were isolated on Todd-Hewitt agar by a step-wise selection procedure. Resistance is defined here as the ability to form colonies in 48 hr. First-step mutants demonstrated six different levels of resistance, ranging from 400 to 1000 micrograms/mL sodium flu...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345870660101701
更新日期：1987-10-01 00:00:00
abstract：:Mandibular prognathism is a common dentofacial phenotype with a substantial genetic component; however, few susceptibility loci have been mapped. Ethnicity is a risk factor for mandibular prognathism, and a relatively high prevalence is observed in Asian populations. The hypothesis of this study suggested that a speci...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/0022034510382546
更新日期：2011-01-01 00:00:00
abstract：:The development of predictive and rapid methods for the assessment of the anticaries activity of topical fluorides has been a long-standing objective of caries researchers. These methods can provide useful benefits in a number of applications, ranging from the identification of novel agents to progress into clinical t...
journal_title：Journal of dental research
pub_type： 杂志文章,评审
doi：10.1177/002203459207100S19
更新日期：1992-04-01 00:00:00
abstract：:Our observations confirm the previously reported observation that multinucleated cells develop within a two-week period in cat bone-marrow cultures. Calcitonin induces cytoplasmic contraction in these multinucleated cells, which indicates that they resemble osteoclasts. After seven days of culture, cat bone-marrow cul...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345860650070201
更新日期：1986-07-01 00:00:00
abstract：:We have investigated the role of the activation of nuclear poly(ADP-ribose) polymerase (PARP) enzyme, a mediator of downstream nitric oxide toxicity, using a combined approach of pharmacological inhibition and genetic disruption in a ligature-induced-periodontitis model in rats and mice. Immunohistochemical analysis r...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/154405910308201210
更新日期：2003-12-01 00:00:00
abstract：:Spontaneously occurring bacteriophage-resistant mutants of Actinomyces viscosus were isolated. The mutants exhibited altered coaggregation patterns with streptococci. From analysis of the data, it appears that a cell surface structure on A. viscosus may function both as a phage receptor and as a binding site for co-ag...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345830620111801
更新日期：1983-11-01 00:00:00
abstract：:Priming with tumor antigens is one of the most important strategies in cancer immunotherapy. To enhance tumor antigenicity, OK-432, a streptococcal preparation, was coupled to squamous cell carcinoma (KLN-205) by means of a 0.2% glutaraldehyde method. The purpose of this study was to investigate whether OK-432-conjuga...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/154405910308200813
更新日期：2003-08-01 00:00:00
abstract：:The number of molars selected for prophylactic removal varies widely among general dental practitioners and oral surgeons. To understand the basis for such variations, we investigated two hypotheses: (1) Individual judgment strategies will differ concerning the use of cues (items of information), and (2) few dentists ...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345000790121101
更新日期：2000-12-01 00:00:00
abstract：:Periostin is a mesenchymal cell marker predominantly expressed in collagen-rich fibrous connective tissues, including heart valves, tendons, perichondrium, periosteum, and periodontal ligament (PDL). Knockdown of periostin expression in mice results in early-onset periodontitis and failure of cardiac healing after acu...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/0022034514543015
更新日期：2014-09-01 00:00:00
abstract：:Cytotoxicity of fine cut amalgam was persistent until 24 hours after trituration in vitro, whereas cytotoxicity of spherical amalgam diminished more quickly. Increasing mercury content in spherical amalgam increased cytotoxicity. Possible reactions for the diminution of cytotoxicity of amalgams with time are discussed...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：
更新日期：1975-03-01 00:00:00
abstract：:The major phosphoprotein in dentin is the aspartic acid and serine-rich protein called dentin phosphophoryn (DPP). DPP appears to be synthesized as a part of a larger compound protein, dentin sialophosphoprotein (DSPP). DSPP has never been isolated or detected in dentin extracts. It is now evident that DSPP is a chime...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/0022034513516631
更新日期：2014-02-01 00:00:00
