Abstract:
:Catecholamines are present in saliva, but their influence on oral epithelium is not understood. Because psychological stress increases salivary catecholamines and impairs oral mucosal wound healing, we sought to determine if epithelial adrenergic signaling could link these two findings. We found that cultured human oral keratinocytes (HOK) express the α(2B)- and β(2)-adrenergic receptors (ARs). Exposure of HOK to either epinephrine or the β-AR agonist, isoproterenol, reduced migratory speed and decreased in vitro scratch wound healing. Incubation with the β-AR antagonist timolol reversed the catecholamine-induced effects, indicating that the observed response is mediated by β-AR. Epinephrine treatment decreased phosphorylation of the mitogen-activated protein kinases (MAPK) ERK1/2 and p38; these decreases were also reversed with timolol. Cultured HOK express enzymes of the epinephrine synthetic pathway, and generate epinephrine. These findings demonstrate that stress-induced elevations of salivary catecholamines signal through MAPK pathways, and result in impaired oral keratinocyte migration required for healing.
journal_name
J Dent Resjournal_title
Journal of dental researchauthors
Steenhuis P,Huntley RE,Gurenko Z,Yin L,Dale BA,Fazel N,Isseroff RRdoi
10.1177/0022034510388034subject
Has Abstractpub_date
2011-02-01 00:00:00pages
186-92issue
2eissn
0022-0345issn
1544-0591pii
0022034510388034journal_volume
90pub_type
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