HEMA enhances IgG1 production by human B-cells in vitro.

Abstract:

UNLABELLED:We have previously shown that the resin monomer 2-hydroxyethylmethacrylate (HEMA) affects mouse B-lymphocyte activity, leading to increased IgG1 antibody production in vivo. In the present study, we tested, in vitro, the hypothesis that HEMA also affects human B-lymphocyte activity. The in vitro production of IgG1, IgM, and IgA in supernatants from purified human CD19+ B-lymphocyte cultures, containing different concentrations of HEMA, was assayed with ELISA. Proliferation was measured by [methyl-(3)H] thymidine incorporation. Of the different HEMA concentrations used, the lower concentrations caused a significant increase in IgG1 production, but not in IgM or IgA production, in vitro. The lower HEMA concentrations did not significantly change B-cell proliferation. At the highest concentration, HEMA significantly suppressed IgG1 and IgM production, as well as B-cell proliferation, in vitro. In conclusion, HEMA can, at certain concentrations, selectively enhance human B-lymphocyte IgG1 production. ABBREVIATIONS:2-hydroxyethylmethacrylate (HEMA), Dulbecco's Modified Eagle's Medium supplemented with heat-inactivated fetal bovine serum, gentamycin, penicillin, and streptomycin (D-MEM++++), Enzyme-linked Immuno-sorbent Assay (ELISA), phosphate-buffered saline (PBS), ovalbumin (OVA), pokeweed mitogen (PWM), counts per minute (CPM).

journal_name

J Dent Res

authors

Andersson J,Dahlgren U

doi

10.1177/0022034510378522

subject

Has Abstract

pub_date

2010-12-01 00:00:00

pages

1461-4

issue

12

eissn

0022-0345

issn

1544-0591

pii

0022034510378522

journal_volume

89

pub_type

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