Abstract：
:Dendritic cells, such as Langerhans cells (LC), in different ectodermal compartments may have different functional capabilities. The present study was undertaken to compare oral Langerhans cells (LC) with those of the epidermis in terms of their ability to co-stimulate T-cells in vitro. A Mixed Epithelial Cell Lymphocyte Reaction (MELR) and a mitogen-driven (concanavalin A) T-cell proliferation assay were used. In both assays, LC in a crude cell suspension of freshly isolated oral epithelial cells were found to be five times more effective in mediating T-cell proliferation than freshly isolated epidermal LC. Twenty-four-hour cell culture at 37 degrees C enhanced the T-cell response in the MELR compared with cells cultured at 4 degrees C. This applied to both skin and oral epithelial cells. Oral and skin epithelial cell suspensions depleted of LC lost the capacity to stimulate allogeneic T-cells. Incubation of the epithelial cell suspensions with recombinant Granulocyte/Macrophage-Colony Stimulating Factor (rGM-CSF) did not enhance the co-stimulating capacity of the LC. Titration of different numbers of oral and skin LC to T-cells showed that skin LC were never able to reach more than 44% of the maximal stimulatory capacity of oral LC. Data show that oral LC are more efficient than skin LC in providing co-stimulatory signals to T-cells, suggesting a difference in functional capacity between the two cell populations.
journal_name
J Dent Resjournal_title
Journal of dental researchauthors
Hasséus B,Jontell M,Bergenholtz G,Eklund C,Dahlgren UIdoi
10.1177/00220345990780030701keywords:
subject
Has Abstractpub_date
1999-03-01 00:00:00pages
751-8issue
3eissn
0022-0345issn
1544-0591journal_volume
78pub_type
杂志文章abstract：:The purpose of this study was to determine the role of saliva-derived biomarkers and periodontal pathogens during periodontal disease progression (PDP). One hundred human participants were recruited into a 12-month investigation. They were seen bi-monthly for saliva and clinical measures and bi-annually for subtractio...
journal_title：Journal of dental research
pub_type： 临床试验,杂志文章
doi：10.1177/0022034511399908
更新日期：2011-06-01 00:00:00
abstract：:Recent advances in molecular biology have facilitated analyses of the oral microbiome ("Who are they?"); however, its functions (e.g., metabolic activities) are poorly understood ("What are they doing?"). This review aims to summarize our current understanding of the metabolism of the oral microbiome. Saccharolytic ba...
journal_title：Journal of dental research
pub_type： 杂志文章,评审
doi：10.1177/0022034515606045
更新日期：2015-12-01 00:00:00
abstract：:Stereologic studies of trabecular architecture suggest that the pig mandibular condyle is strongest when loaded supero-inferiorly, and that stress is concentrated in the antero-inferior region (Teng and Herring, 1995). To test these hypotheses, we investigated the uni-axial mechanical properties of 22 pig mandibular c...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345960750110501
更新日期：1996-11-01 00:00:00
abstract：:Molar teeth of four groups of Osborne-Mendel rats, kept on a cariogenic diet, were treated once per day for 48 days with either distilled water (solution C), a solution containing 0.05% chlorhexidine gluconate and 0.04% NaF, pH 5.8 (solution CXF), CXF plus 1000 ppm Sr (solution CXFS), or CXFS plus 1000 ppm Zn (solutio...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345840630100401
更新日期：1984-10-01 00:00:00
abstract：:This case-control study evaluated the association of headache and other co-morbid pain with temporomandibular disorder (TMD) pain in adolescents and explored the temporal co-variance of headache and TMD pain. In a population-based sample of 12- to 19-year-olds, 350 patients with self-reported TMD pain and 350 healthy ...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/0022034513496255
更新日期：2013-09-01 00:00:00
abstract：:Dimensional alterations of the facial bone wall following tooth extractions in the esthetic zone have a profound effect on treatment outcomes. This prospective study in 39 patients is the first to investigate three-dimensional (3D) alterations of facial bone in the esthetic zone during the initial 8 wks following flap...
journal_title：Journal of dental research
pub_type： 临床试验,杂志文章
doi：10.1177/0022034513506713
更新日期：2013-12-01 00:00:00
abstract：:The range of substrates transported by the sugar-binding protein-dependent msm (multiple sugar metabolism) system of S. mutans was investigated. By determining the ability of unlabeled sugar to compete with radiolabeled melibiose transport, we have demonstrated that the transported sugars included a number of carbohyd...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345930720100701
更新日期：1993-10-01 00:00:00
abstract：:Dissolution of mercury from the Ag-Hg matrix phase of dental amalgam is distilled water and synthetic saliva, and the mercury evaporation from the solutions, were studied in vitro. The main objectives of the study were to evaluate the factors that affect the results of the mercury release tests, and to consider the po...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345900690050901
更新日期：1990-05-01 00:00:00
abstract：:Periostin is a mesenchymal cell marker predominantly expressed in collagen-rich fibrous connective tissues, including heart valves, tendons, perichondrium, periosteum, and periodontal ligament (PDL). Knockdown of periostin expression in mice results in early-onset periodontitis and failure of cardiac healing after acu...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/0022034514543015
更新日期：2014-09-01 00:00:00
abstract：:The kinetics of subsurface demineralization of tooth sections has been studied in real-time by scanning microradiography (SMR). Demineralization was carried out: (1) with a large volume of solution buffered to pH = 4 to maintain a constant composition; and (2) in a small constant volume (approximately 3 mL), buffered ...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345930720051401
更新日期：1993-05-01 00:00:00
abstract：:The filtration and diffusion of tritiated water through dentin disks were measured ina split-chamber diffusion cell. The dentin had been cut with a diamond disk and the surfaces modified with a carbide fissure bur or diamond bur. Disks were given a secondary burnishing treatment with a blank bur or a modified blank bu...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345810600061101
更新日期：1981-06-01 00:00:00
abstract：:Although a secular decline is being seen in coronal caries in children in industrialized countries, there appears to be a relatively high prevalence of root caries, with chronologically older adults exhibiting the highest prevalences. This paper reviews the descriptive and analytical epidemiology of root caries as fou...
journal_title：Journal of dental research
pub_type： 杂志文章,评审
doi：10.1177/00220345900690051901
更新日期：1990-05-01 00:00:00
abstract：:Adhesives cured under constrained conditions develop contraction stresses. We hypothesized that, with dentin as a bonding substrate, the stress would reach a maximum, followed by a continuous decline. Stress development was determined with a tensilometer for two total-etch systems and two systems with self-etching pri...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/154405910608500808
更新日期：2006-08-01 00:00:00
abstract：:Stress analyses are presented for a second mandibular premolar with a class 1 amalgam restoration. The effects of amalgam setting and thermal expansions are examined. An axisymmetric representation of the structure is used and the analyses are carried out by the finite element method. Lined and unlined restorations ar...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345780570051101
更新日期：1978-05-01 00:00:00
abstract：:TWIST1 plays a crucial role in dentinogenesis, and its activity depends on both a dimerization partner selection and phosphorylation. Other factors, like Id proteins, can affect the availability of dimerization partners for TWIST1, subsequently leading to diverse biological outcomes. The purpose of this study was to e...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/0022034514530164
更新日期：2014-06-01 00:00:00
abstract：:The oral microbiome is one of the most stable ecosystems in the body and yet the reasons for this are still unclear. As well as being stable, it is also highly diverse which can be ascribed to the variety of niches available in the mouth. Previous studies have focused on the microflora in disease-either caries or peri...
journal_title：Journal of dental research
pub_type： 杂志文章,评审
doi：10.1177/0022034520915486
更新日期：2020-06-01 00:00:00
abstract：:Dentin sialoprotein (DSP) and dentin phosphoprotein (DPP) are expressed as a single mRNA transcript coding for a large precursor protein termed dentin sialophosphoprotein (DSPP). DSP, DPP, and DSPP have been considered to be tooth-specific. To test for the expression of the dspp gene in bone, we performed Western immu...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/154405910208100607
更新日期：2002-06-01 00:00:00
abstract：:The effect of combined administration of chlorhexidine and fluoride in the drinking water of hamsters on plaque and caries prevention was studied. Plaque indices of the hamsters receiving chlorhexidine either alone or simultaneously with fluoride were significantly lower than those of the control and fluoride only tre...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345770560111401
更新日期：1977-11-01 00:00:00
abstract：:This paper discusses loosely bound fluoride and its role in dental caries and prevention. Loosely bound fluoride (abbr. by Fa) is fluoride adsorbed onto enamel mineral crystallites. Several recent studies indicate that a high total level of fluoride in enamel does not guarantee protection against caries. This leads to...
journal_title：Journal of dental research
pub_type： 杂志文章,评审
doi：10.1177/00220345900690S118
更新日期：1990-02-01 00:00:00
abstract：:The surface enamel of fetal bovine teeth was stained with GBHA to indicate the position of bands of smooth-ended and ruffle-ended ameloblasts relative to the developing enamel. The boundaries of the bands were scored, under a dissecting microscope, and the bulk enamel under each band was collected. The enamel samples ...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345880670060901
更新日期：1988-06-01 00:00:00
abstract：:A single logistic curve was fitted to serial data for three cranial base lengths (S-N, Ba-N, and Ba-S) in 33 boys and 26 girls. Elongation during pubescence plus adolescence is greater in boys than girls for each length. The maximum rate of pubescent elongation occurs earliest in Ba-N. There are no consistent sex diff...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345770560071501
更新日期：1977-07-01 00:00:00
abstract：:The biocompatibility of two implant materials, vitallium and poly-methyl methacrylate (PMMA), was evaluated in an animal model in which bone formation and resorption are independent of each other. Neither material accelerated resorption, but formation was inhibited 12% by vitallium and 38% by PMMA. When three PMMA pre...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345810600010801
更新日期：1981-01-01 00:00:00
abstract：:Disks of dentin taken from extracted human teeth demonstrated the optical property of the magnification/reduction effect. When images were transmitted from a pulpal-to-occlusal direction, the images were magnified. When the disks were inverted, the images were reduced in size. The images were slightly distorted but cl...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345760550041601
更新日期：1976-07-01 00:00:00
abstract：:We explored the potential to diagnose Zika virus (ZIKV) infection by analyzing peptides in saliva during a convalescent phase of infection, long after resolution of acute disease. A 25-y-old woman clinically diagnosed with Zika fever in the first trimester was enrolled with her dizygotic twins for a 3-mo postnatal sam...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/0022034517723325
更新日期：2017-09-01 00:00:00
abstract：:Genetic approaches are rapidly yielding new information about our sense of taste. This information comes from both molecular studies of genes encoding taste receptors and other taste-signaling components, and from studies of inherited variation in taste abilities. Our understanding of bitter taste has advanced by comb...
journal_title：Journal of dental research
pub_type： 杂志文章,评审
doi：10.1177/154405910408300603
更新日期：2004-06-01 00:00:00
abstract：:This study demonstrated that human submandibular-sublingual saliva (HSMSL) provided a better substrate than did whole saliva or parotid saliva for the binding of Streptococcus sanguis in a glass adherence assay. Additional evidence indicated that the lower molecular weight salivary mucin in HSMSL was involved in these...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345820610120101
更新日期：1982-12-01 00:00:00
abstract：:Intracystic fluid pressure is thought to be involved in odontogenic cyst growth. In this study, we investigated the effects of positive pressure on the expression of interleukin-1alpha (IL-1alpha), matrix metalloproteinases (MMPs), and prostaglandin E2 (PGE2) in odontogenic keratocysts to determine whether this pressu...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/154405910508401008
更新日期：2005-10-01 00:00:00
abstract：:Previous studies show that both activin and Bmp4 act as crucial mesenchymal odontogenic signals during early tooth development. Remarkably, mice lacking activin-βA ( Inhba-/-) and mice with neural crest-specific inactivation of Bmp4 ( Bmp4ncko/ncko) both exhibit bud-stage developmental arrest of the mandibular molar t...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/0022034517713710
更新日期：2017-09-01 00:00:00
abstract：:The effect of a topical fluoride regimen consisting of a four-minute pre-treatment with a saturated dicalcium phosphate dihydrate (DCPD) solution followed by a four-minute application of APF was compared with that of a four-minute application of APF alone with respect to the deposition of fluoride in enamel in an apat...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345860650030201
更新日期：1986-03-01 00:00:00
abstract：:Minimizing patient exposure while maintaining a diagnostically acceptable radiograph is a major goal in diagnostic radiography. Rare-earth filters may be the means to achieve this goal due to their "band-pass effect". The purpose of this study was to examine the image contrast effects and exposure reductions for vario...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345870660080701
更新日期：1987-08-01 00:00:00
