Reduced antigenicity of type I collagen and proteoglycans in sclerotic dentin.

Abstract:

:Antigenic alterations to the dentin organic matrix may be detected by an immunohistochemical approach. We hypothesized that alterations in the antigenicity of type I collagen and proteoglycans occur in sclerotic dentin under caries lesions. Transverse sections were prepared from carious teeth in the sclerotic zone and normal hard dentin. A double-immunolabeling technique was performed on these sections, with anti-type I collagen and anti-chondroitin 4/6 sulfate monoclonal primary antibodies. We used gold-conjugated secondary antibodies to visualize the distribution of intact collagen fibrils and proteoglycans by high-resolution SEM. For sclerotic dentin, labeling densities were 19.57 +/- 3.01/microm2 for collagen and 9.84 +/- 2.62/microm2 for proteoglycans. For normal hard dentin, values were 35.20 +/- 2.73/microm2 and 17.03 +/- 1.98/microm2, respectively. Distribution of intact collagen fibrils and proteoglycans in sclerotic dentin was significantly lower than in normal hard dentin. Reductions in antigenicity from the organic matrix of sclerotic dentin under caries lesions raise concern about the potential of intrafibrillar remineralization.

journal_name

J Dent Res

authors

Suppa P,Ruggeri A Jr,Tay FR,Prati C,Biasotto M,Falconi M,Pashley DH,Breschi L

doi

10.1177/154405910608500204

keywords:

subject

Has Abstract

pub_date

2006-02-01 00:00:00

pages

133-7

issue

2

eissn

0022-0345

issn

1544-0591

pii

85/2/133

journal_volume

85

pub_type

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