Abstract：
:Adenoviral vectors effectively transfer genes to rat salivary glands. However, potent immune responses limit their use in vivo. Mice offer more opportunities than rats for the study of these immune processes. We first established conditions for infection of mouse salivary glands, with an adenoviral vector. The effects of time, viral dose, viral diluent buffer volume, and dexamethasone on expression of a transgene, luciferase, were determined by means of the recombinant vector AdCMVluc. Optimal luciferase expression was observed when the vector was suspended in 50 microL of buffer. This volume completely filled the gland parenchyma and slightly distended the capsule. Dexamethasone increased immediate transgene expression and reduced the acute inflammation one day following viral administration, but did not alter subsequent mononuclear inflammation or transgene expression 14 or 28 days later. An adenoviral vector encoding either anti-inflammatory cytokine IL-4 or IL-10 was co-administered with AdCMVluc to increase transgene expression at 14 and 28 days. While this strategy did not extend the duration of luciferase expression, co-administration of AdCMVIL-10 with AdCMVluc almost completely eliminated the chronic inflammatory infiltrate in the glands after 28 days. This study demonstrates that adenoviral-mediated gene transfer to mouse submandibular glands is possible by intraductal cannulation and that reduction of either the acute or chronic inflammatory infiltrates was insufficient to increase long-term transgene expression in this tissue.
journal_name
J Dent Resjournal_title
Journal of dental researchauthors
Wang S,Baum BJ,Yamano S,Mankani MH,Sun D,Jonsson M,Davis C,Graham FL,Gauldie J,Atkinson JCdoi
10.1177/00220345000790020201keywords:
subject
Has Abstractpub_date
2000-02-01 00:00:00pages
701-8issue
2eissn
0022-0345issn
1544-0591journal_volume
79pub_type
杂志文章abstract：:The Kubelka-Munk (K-M) theory provides a reflectance model for translucent materials placed on backings of different colors. We hypothesize that Kubelka-Munk (K-M) theoretical diffuse reflectance spectra of dentin and enamel are in good agreement with observed diffuse reflectance. The aim of this study was to measure ...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345010800020901
更新日期：2001-02-01 00:00:00
abstract：:This study was conducted to test the hypothesis that physiological changes which occur during aging increase the biological impact of fluoride and reduce the threshold of safe fluoride exposure. Four groups of rats were fed a low-fluoride diet (< 1.2 ppm) ad libitum and received 0, 5, 15, or 50 ppm fluoride in their d...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345950740011201
更新日期：1995-01-01 00:00:00
abstract：:Recent advances in molecular biology have facilitated analyses of the oral microbiome ("Who are they?"); however, its functions (e.g., metabolic activities) are poorly understood ("What are they doing?"). This review aims to summarize our current understanding of the metabolism of the oral microbiome. Saccharolytic ba...
journal_title：Journal of dental research
pub_type： 杂志文章,评审
doi：10.1177/0022034515606045
更新日期：2015-12-01 00:00:00
abstract：:Loss of hybrid layer integrity compromises resin-dentin bond stability. Matrix metalloproteinases (MMPs) may be partially responsible for hybrid layer degradation. Since chlorhexidine inhibits MMPs, we hypothesized that chlorhexidine would decelerate the loss of resin-dentin bonds. Class I preparations in extracted th...
journal_title：Journal of dental research
pub_type： 杂志文章,随机对照试验
doi：10.1177/154405910708600115
更新日期：2007-01-01 00:00:00
abstract：:microRNAs could be mechanosensitive and emerge as critical posttranscriptional regulators in the bone-remodeling process. During orthodontic tooth movement (OTM), the application of mechanical force induces alveolar bone remodeling, but whether microRNAs respond to orthodontic force and contribute to OTM is unknown. m...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/0022034516657043
更新日期：2016-11-01 00:00:00
abstract：:Weanling rice rats were fed either standard laboratory chow or Diet 2000. The animals were sacrificed at 4, 8, 12, and 15 weeks. There was an increasing amount of exposed root surface and root surface caries with age in the animals fed Diet 2000. There was exposed root surface but no caries in the animals fed laborato...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345770560091701
更新日期：1977-09-01 00:00:00
abstract：:Estrogen deficiency results in disruption of maxillary alveolar bone microarchitecture. Most of the actions of estrogen in long bones occur via estrogen receptor α (ERα). However, the function of ERα in the maxillary bone has not been defined. We aimed to investigate the role and underlying mechanisms of ERα in the ph...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/0022034516633154
更新日期：2016-06-01 00:00:00
abstract：:Secondary palatogenesis occurs when the bilateral palatal shelves (PS), arising from maxillary prominences, fuse at the midline, forming the hard and soft palate. This embryonic phenomenon involves a complex array of morphogenetic events that require coordinated proliferation, apoptosis, migration, and adhesion in the...
journal_title：Journal of dental research
pub_type： 杂志文章,评审
doi：10.1177/0022034517726284
更新日期：2017-10-01 00:00:00
abstract：:This study describes the formation of a calcium polyacrylate-hydroxyapatite cement. Our hypothesis was that calcium phosphates would rapidly hydrolyze in the presence of polyacrylic acid (PAA) to form a cement. PAA, tetracalcium phosphate (TetCP), and dicalcium phosphate (DCP) were reacted together and formed calcium ...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345940730030501
更新日期：1994-03-01 00:00:00
abstract：:Dental caries is a microbially mediated disease that can result in significant tooth structure degradation. Although the preponderance of lesions is treated by surgical intervention, various strategies have been developed for its noninvasive management. Here, we use a novel approach for noninvasive treatment based on ...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/0022034515619370
更新日期：2016-03-01 00:00:00
abstract：:The aims of this work were (a) to assess the oral bioavailability of fluoride delivered from dentifrices and (b) to test for a possible link between the results and clinical data obtained with the same dentifrices. Oral fluoride concentrations were measured in samples of saliva and plaque taken from seven subjects, af...
journal_title：Journal of dental research
pub_type： 临床试验,杂志文章,评审
doi：10.1177/002203459207100S09
更新日期：1992-04-01 00:00:00
abstract：:Not only are teeth essential for mastication, but also missing teeth are considered a social handicap due to speech and aesthetic problems, with a resulting high impact on emotional well-being. Several treatment procedures are currently available for tooth replacement with mostly inert prosthetic materials and implant...
journal_title：Journal of dental research
pub_type： 杂志文章,评审
doi：10.1177/0022034509355440
更新日期：2010-02-01 00:00:00
abstract：:Phosphatases are involved in bone and tooth mineralization, but their mechanisms of action are not completely understood. Tissue-nonspecific alkaline phosphatase (TNAP, ALPL) regulates inhibitory extracellular pyrophosphate through its pyrophosphatase activity to control mineral propagation in the matrix; mice without...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/0022034513490958
更新日期：2013-08-01 00:00:00
abstract：:The purpose of this investigation was to determine the water sorption and solubility, as well as the filler composition and extent of chemical degradation in water, of eight different posterior composites. With one exception, the materials with the largest quantity of fillers had the least water sorption and solubilit...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345860650110601
更新日期：1986-11-01 00:00:00
abstract：:Dental composites develop residual stresses during polymerization due to shrinkage. These stresses may change with time because of relaxation and water sorption in the oral environment. This phenomenon is likely dependent on the composition of the materials, specifically their hydrophilic characteristics, and could re...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/0022034513513905
更新日期：2014-02-01 00:00:00
abstract：:Dynamic changes of pulpal blood flow (PBF) and gingival blood flow (GBF) induced by intra-venous injection of two kinds of vasoactive drugs were observed in dogs by means of Laser Doppler Velocimetry. Intra-venous injection of norepinephrine caused PBF to increase, corresponding to the blood pressure (BP) increase, wh...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345890680050701
更新日期：1989-05-01 00:00:00
abstract：:The ability of physiological amounts of lysozyme to de-chain two serotype c strains of Streptococcus mutans was determined. Both human and hen lysozymes were equally effective in chain breakage of S. mutans DPR and S. mutans DJR. De-chaining did not affect growth of cultures, but resulted in finely dispersed suspensio...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345850640010901
更新日期：1985-01-01 00:00:00
abstract：:The effects of Escherichia coli endotoxin upon mouse L929 cell proliferation, DNA synthesis, protein synthesis, and proline incorporation were determined. It was found that a level of endotoxin which inhibited cell proliferation prompted a similar inhibition of DNA synthesis and overall cell protein synthesis. In cont...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345790580061301
更新日期：1979-06-01 00:00:00
abstract：:The resin-dentin interdiffusion zone produced by a dentin-adhesive system that removes the smear layer and concurrently decalcifies superficial dentin was morphologically examined by both scanning and transmission electron microscopy. Cross-sectioned resin-bonded dentin discs were etched with an argon-ion beam to make...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345930720020501
更新日期：1993-02-01 00:00:00
abstract：:The hypothesis that periodontal disease is associated with pre-term low birthweight was tested in a prospective follow-up study of rural prima-gravida women (N = 227) who were free of tobacco, alcohol, and drug use. Women with 3rd trimester mean probing pocket depths, plaque, and bleeding scores that were greater than...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/154405910508400313
更新日期：2005-03-01 00:00:00
abstract：:This study investigated the relevance between pulp vitality and autophagy in aged human dental pulp cells (HDPCs) and whether peroxisome proliferator-activated receptor gamma (PPARγ) affects autophagy regulation for homeostasis in the aging progress. In vivo experiments were used in human and Sprague-Dawley rat teeth ...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/0022034515573833
更新日期：2015-05-01 00:00:00
abstract：:Significant inhibition of PMN metabolic activity by F- occurred at 0.1, 0.5 and 0.0 mM F- for O2- generation, 1-C14 CO2 release labeled glucose and NBT-reduction respectively. This inhibition resulted primarily from F- suppression of nonoxidative glucose metabolism. As the concentration of F- in the O2- generating sys...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345790580092101
更新日期：1979-09-01 00:00:00
abstract：:Previous reports have linked the prevalence of tooth abnormalities with high caries experience in the different types of epidermolysis bullosa (EB). However, it is not known to what extent the apparent susceptibility to enamel caries is due to disease-related altered enamel chemistry in these cases. The aim of this st...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345960750090901
更新日期：1996-09-01 00:00:00
abstract：:A radiotracer technique was used to study silver and gold diffusion into dental porcelain under experimental conditions close to the real conditions in prosthetic laboratories for porcelain bakes. It was clearly shown that these non-oxidizable elements were able to diffuse into the ceramic as well as oxidizable ones. ...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345870660120401
更新日期：1987-12-01 00:00:00
abstract：:Conservative techniques for improving the appearance of discolored teeth have become popular in the past decade. These include: in-office bleaching with 30% hydrogen peroxide, which is applied on etched enamel with a gauze pad and then exposed to a bleaching light; home bleaching with a mild form of peroxide, such as ...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345930720011001
更新日期：1993-01-01 00:00:00
abstract：:Extracellular vesicles (EVs), several tens to hundreds of nanometers in size, are vesicles secreted by cells for intercellular communication. EVs released from mesenchymal stem cells (MSC-EVs) have the potential to treat multiple diseases. This study aimed to determine the effects of MSC-EVs on bisphosphonate-related ...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/0022034520906793
更新日期：2020-05-01 00:00:00
abstract：:Clinical studies have shown that metabolic syndrome (MetS) is associated with increased risk of developing periodontitis. However, the underlying mechanisms remain largely unknown. Since it is known that lipopolysaccharide (LPS)-activated toll-like receptor 4 signaling pathways play a crucial role in periodontitis, we...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/0022034514561658
更新日期：2015-02-01 00:00:00
abstract：:Tempering of dental porcelain by forced convective cooling in air has been shown previously to inhibit the sizes of cracks induced in porcelain surfaces. However, the depth of compressive stress that is produced by tempering of feldspathic porcelain has not been determined. It can be assumed that the potential of temp...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345910700020701
更新日期：1991-02-01 00:00:00
abstract：:In an attempt to make a dynamic study of the isoelectric point and the chargeability of human dental enamel, an electrochemical approach was utilized. Zeta potential of three kinds of the enamel was measured by a streaming potential method. As a result, these isoelectric points could be decided. ...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345780570022701
更新日期：1978-02-01 00:00:00
abstract：:While dental pulp appears to be able to form mineralized matrices that do not always resemble dentin, the precise characteristics of the hard tissue and the mechanism of its induction remain unknown. Therefore, we evaluated hard tissue induced by transplantation of pulp into subcutaneous tissue. Seven days after trans...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/154405910708600515
更新日期：2007-05-01 00:00:00
