Bone morphogenetic protein-2 inhibits differentiation and mineralization of cementoblasts in vitro.

Abstract:

:As an approach for improving the outcome and predictability of periodontal regenerative therapies, we have focused on determining the responses of cells within the local environment to putative regenerative factors. This study examined the effects of bone morphogenetic protein-2 (BMP-2) on murine cementoblasts in vitro. Northern blot analysis indicated that BMP-2 decreased mRNA levels of bone sialoprotein and type I collagen dose-dependently (10-300 ng/mL). At low doses, up to 100 ng/mL, BMP-2 had no effect on transcripts for osteocalcin and osteopontin, whereas at 300 ng/mL, BMP-2 greatly increased expression of these two genes. BMP-2 also inhibited cementoblast-mediated mineral nodule formation in a dose-dependent manner (inhibition was noted at 10 ng/mL). Noggin reversed the effects of BMP-2 on gene expression and on mineralization. These findings reflect the diverse responses of periodontal cells to BMP-2 and highlight the need to consider the complexity of factors involved in designing predictable regenerative therapies.

journal_name

J Dent Res

authors

Zhao M,Berry JE,Somerman MJ

doi

10.1177/154405910308200106

keywords:

subject

Has Abstract

pub_date

2003-01-01 00:00:00

pages

23-7

issue

1

eissn

0022-0345

issn

1544-0591

journal_volume

82

pub_type

杂志文章
  • Activin and Bmp4 Signaling Converge on Wnt Activation during Odontogenesis.

    abstract::Previous studies show that both activin and Bmp4 act as crucial mesenchymal odontogenic signals during early tooth development. Remarkably, mice lacking activin-βA ( Inhba-/-) and mice with neural crest-specific inactivation of Bmp4 ( Bmp4ncko/ncko) both exhibit bud-stage developmental arrest of the mandibular molar t...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/0022034517713710

    authors: Kwon HE,Jia S,Lan Y,Liu H,Jiang R

    更新日期:2017-09-01 00:00:00

  • Urinary catecholamine levels in children with and without dental caries.

    abstract::Urinary catecholamines have been used to measure emotionally stressful states which may affect the development of dental caries. This study investigates the hypothesis that children with and without dental caries do not differ significantly in the mean values of urinary catecholamines such as epinephrine, norepinephri...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345950740100801

    authors: Vanderas AP,Manetas C,Papagiannoulis L

    更新日期:1995-10-01 00:00:00

  • Effects of rare-earth filters on patient exposure and image contrast.

    abstract::Minimizing patient exposure while maintaining a diagnostically acceptable radiograph is a major goal in diagnostic radiography. Rare-earth filters may be the means to achieve this goal due to their "band-pass effect". The purpose of this study was to examine the image contrast effects and exposure reductions for vario...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345870660080701

    authors: Mauriello SM,Washburn DB,Matteson SR

    更新日期:1987-08-01 00:00:00

  • Effects of positive pressure in odontogenic keratocysts.

    abstract::Intracystic fluid pressure is thought to be involved in odontogenic cyst growth. In this study, we investigated the effects of positive pressure on the expression of interleukin-1alpha (IL-1alpha), matrix metalloproteinases (MMPs), and prostaglandin E2 (PGE2) in odontogenic keratocysts to determine whether this pressu...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/154405910508401008

    authors: Oka S,Kubota Y,Yamashiro T,Ogata S,Ninomiya T,Ito S,Shirasuna K

    更新日期:2005-10-01 00:00:00

  • Role of Oxidized Lipids and TRP Channels in Orofacial Pain and Inflammation.

    abstract::Acute or chronic inflammation comprises a highly prevalent type of orofacial pain and is mediated by the generation of endogenous agonists that activate numerous receptors expressed on terminals of trigeminal (TG) nociceptive afferent neurons. One such studied receptor is transient receptor potential vanilloid subtype...

    journal_title:Journal of dental research

    pub_type: 杂志文章,评审

    doi:10.1177/0022034516653751

    authors: Hargreaves KM,Ruparel S

    更新日期:2016-09-01 00:00:00

  • Impact of Carbamide Peroxide Whitening Agent on Dentinal Collagen.

    abstract::Carbamide peroxide (CP) is widely used as a tooth-whitening agent in self-administered tooth-bleaching products. In this study, the effects of 5% and 10% CP on dentinal collagen structure and chemical properties were evaluated in vitro. Thirty-five intact teeth were exposed to 2 whitening protocols (2 or 4 h daily) wi...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/0022034518822826

    authors: Redha O,Strange A,Maeva A,Sambrook R,Mordan N,McDonald A,Bozec L

    更新日期:2019-04-01 00:00:00

  • Limitations in bonding to dentin and experimental strategies to prevent bond degradation.

    abstract::The limited durability of resin-dentin bonds severely compromises the lifetime of tooth-colored restorations. Bond degradation occurs via hydrolysis of suboptimally polymerized hydrophilic resin components and degradation of water-rich, resin-sparse collagen matrices by matrix metalloproteinases (MMPs) and cysteine ca...

    journal_title:Journal of dental research

    pub_type: 杂志文章,评审

    doi:10.1177/0022034510391799

    authors: Liu Y,Tjäderhane L,Breschi L,Mazzoni A,Li N,Mao J,Pashley DH,Tay FR

    更新日期:2011-08-01 00:00:00

  • Mouse cellular cementum is highly dependent on growth hormone status.

    abstract::Cementum is known to be growth-hormone (GH)-responsive, but to what extent is unclear. This study examines the effects of extremes of GH status on cementogenesis in three lines of genetically modified mice; GH excess (giant), GH antagonist excess (dwarf), and GH receptor-deleted (GHR-KO) (dwarf). Age-matched mandibula...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/154405910408300107

    authors: Smid JR,Rowland JE,Young WG,Daley TJ,Coschigano KT,Kopchick JJ,Waters MJ

    更新日期:2004-01-01 00:00:00

  • The extent of the odontoblast process in human dentin.

    abstract::Impacted human third molar teeth were examined, both with the light and electron microscope, to determine the extent of the odontoblast process. The odontoblast process was found to be limited to the inner third of coronal dentin and inner half of mid-root dentin. ...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/002203457905800412011

    authors: Thomas HF

    更新日期:1979-11-01 00:00:00

  • Radiopaque Tagging Masks Caries Lesions following Incomplete Excavation in vitro.

    abstract::One-step incomplete excavation seals caries-affected dentin under a restoration and appears to be advantageous in the treatment of deep lesions. However, it is impossible to discriminate radiographically between intentionally left, arrested lesions and overlooked or active lesions. This diagnostic uncertainty decrease...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/0022034514531291

    authors: Schwendicke F,Meyer-Lueckel H,Schulz M,Dörfer CE,Paris S

    更新日期:2014-06-01 00:00:00

  • Water aging reverses residual stresses in hydrophilic dental composites.

    abstract::Dental composites develop residual stresses during polymerization due to shrinkage. These stresses may change with time because of relaxation and water sorption in the oral environment. This phenomenon is likely dependent on the composition of the materials, specifically their hydrophilic characteristics, and could re...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/0022034513513905

    authors: Park JW,Ferracane JL

    更新日期:2014-02-01 00:00:00

  • Transport of sugars, including sucrose, by the msm transport system of Streptococcus mutans.

    abstract::The range of substrates transported by the sugar-binding protein-dependent msm (multiple sugar metabolism) system of S. mutans was investigated. By determining the ability of unlabeled sugar to compete with radiolabeled melibiose transport, we have demonstrated that the transported sugars included a number of carbohyd...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345930720100701

    authors: Tao L,Sutcliffe IC,Russell RR,Ferretti JJ

    更新日期:1993-10-01 00:00:00

  • Transcriptome Reveals Cathepsin K in Periodontal Ligament Differentiation.

    abstract::Periodontal ligaments (PDLs) play an important role in remodeling the alveolar bond and cementum. Characterization of the periodontal tissue transcriptome remains incomplete, and an improved understanding of PDL features could aid in developing new regenerative therapies. Here, we aimed to generate and analyze a large...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/0022034516645796

    authors: Yamada S,Ozaki N,Tsushima K,Yamaba S,Fujihara C,Awata T,Sakashita H,Kajikawa T,Kitagaki J,Yamashita M,Yanagita M,Murakami S

    更新日期:2016-08-01 00:00:00

  • A comparison of antimicrobial activity of four disclosant dyes.

    abstract::Four disclosant dyes were tested for antimicrobial activity against 27 oral reference strains and five non-oral human pathogens. Erythrosine and fluorescein inhibited most gram-positive and gram-negative organisms associated with dental plaque, and were bactericidal to selected strains. Fast green and brilliant blue d...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345830620071601

    authors: Baab DA,Broadwell AH,Williams BL

    更新日期:1983-07-01 00:00:00

  • Effects of various resin composite (co)monomers and extracts on two caries-associated micro-organisms in vitro.

    abstract::Previous studies have shown that extracts of various filling materials, e.g., resin composites, may influence the growth of cariogenic micro-organisms. The purpose of this study was to examine the effects of important resin composite (co)monomers (Bis-GMA, UDMA, TEGDMA, EGDMA) and extracts of two commercial dental com...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345980770010601

    authors: Hansel C,Leyhausen G,Mai UE,Geurtsen W

    更新日期:1998-01-01 00:00:00

  • Temperature ofthe pulp chamber during impression taking of full crown preparations with modelling compound.

    abstract::Temperature elevations were measured in the pulp chamber of full cast crown preparations during impression taking with modelling compound in copper bands. Uncontrolled flaming, controlled flaming, and water heating of the bands produced temperatures of 53, 44, and 41.5 C, respectively. The time involved in restoring t...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:

    authors: Grajower R,Kaufman E,Stern N

    更新日期:1975-03-01 00:00:00

  • Analysis of tempering stresses in metal-ceramic disks.

    abstract::Previous studies showed that residual compressive stresses induced by thermal tempering retarded the growth of surface cracks in bilayered porcelain disks. The objectives of the present study were: (1) to determine whether thermal tempering by air blasting reduces the length of cracks induced by microhardness indentat...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345960750020301

    authors: DeHoff PH,Anusavice KJ,Vontivillu SB

    更新日期:1996-02-01 00:00:00

  • Impact of endodontic treatments on the rigidity of the root.

    abstract::The destabilizing effect of endodontic treatment upon teeth is still controversial. The purpose of this study was to investigate the effects of different steps of endodontic treatments upon the rigidity of teeth. Extracted untreated central maxillary anterior teeth were loaded (3.75 N), and deformations of the root we...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/154405910608500416

    authors: Lang H,Korkmaz Y,Schneider K,Raab WH

    更新日期:2006-04-01 00:00:00

  • A longitudinal evaluation of fissure sealants applied in dental practices.

    abstract::Sealants are highly effective in preventing dental caries in the pits and fissures of teeth when applied by trained operators in clinical trials and public health programs. The effectiveness of fissure sealants when applied in dental practices is still not known. The purpose of this longitudinal study was to evaluate ...

    journal_title:Journal of dental research

    pub_type: 临床试验,杂志文章

    doi:10.1177/00220345950740091301

    authors: Ismail AI,Gagnon P

    更新日期:1995-09-01 00:00:00

  • A new type of hydraulic dental cement.

    abstract::A new type of hydraulic cement has been developed. This cement consists of a mixture of cement powder and setting agent powder and therefore has only to be kneaded with a little amount of water when being used. ...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345790580050601

    authors: Kohmura TT,Ida K

    更新日期:1979-05-01 00:00:00

  • Morphologic and biochemical changes of the masseter muscles induced by occlusal wear: studies in a rat model.

    abstract::Occlusal alterations may result in changes in the functional performance of masticatory muscles. In this study, we set up an experimental model in rats to examine whether masticatory muscle abnormalities occur after a malocclusion is induced. Rats underwent unilateral amputation of the molar cusps to simulate an occlu...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345990780111101

    authors: Bani D,Bani T,Bergamini M

    更新日期:1999-11-01 00:00:00

  • Ready-to-eat cereals and dental caries in children: a three-year study.

    abstract::In a three-year study of 145 adolescent males in a boarding school in Wisconsin, there were no statistically significant differences in dental caries experience between a group consuming phosphates added to pre-sweetened and non-presweetened ready-to-eat cereals, and the control group, or between cereal eaters and non...

    journal_title:Journal of dental research

    pub_type: 临床试验,杂志文章

    doi:10.1177/00220345790580090201

    authors: Wilson CJ

    更新日期:1979-09-01 00:00:00

  • Masticatory muscle silent periods in patients with MPD syndrome before and after treatment.

    abstract::Masticatory muscle silent periods were compared in normal subjects and in patients with MPD syndrome before and after a variety of psychological, physiological, and pharmacological forms of therapy. All patients initially showed prolonged silent periods in one or more of the muscles. There was a direct relationship be...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345810600030501

    authors: Skiba TJ,Laskin DM

    更新日期:1981-03-01 00:00:00

  • Randomized Clinical Trial on Sodium Fluoride with Tricalcium Phosphate.

    abstract::This 24-mo randomized controlled trial was based on a double-blind parallel design, and it compared the effectiveness of 2 fluoride application protocols in arresting dentine caries in primary teeth. Three-year-old children with active dentine caries were recruited and randomly allocated to 2 treatment groups. Childre...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/0022034520952031

    authors: Chen KJ,Gao SS,Duangthip D,Lo ECM,Chu CH

    更新日期:2021-01-01 00:00:00

  • Thermal stimulation of the oral mucosa and nasal airway resistance.

    abstract::A procedure for measuring nasal airway resistance using posterior rhinorheometry is described. Baseline nasal airway resistances +/- 1 S.D. at an airflow of 0.5 L/sec were found to be 1.077 +/- 0.683 cm H2O for inhalation and 1.359 +/- 0.792 cm H2O for exhalation. Application of either cold water (4 to 6 C) or warm wa...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345770560110301

    authors: Martin JS,Tansy MF

    更新日期:1977-11-01 00:00:00

  • Storage of human periodontal ligament cells in a combination of different media.

    abstract::Different types of storage media have been studied for use in the replantation of exarticulated teeth. It was found that human periodontal ligament cells survived well in milk. Fifty percent of the cells were viable after 12 hours' storage in milk, while no cells were viable after three hours' storage in saliva. Brief...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345810600111301

    authors: Blomlöf L

    更新日期:1981-11-01 00:00:00

  • Fluoride deposition in human enamel in vivo from professionally applied fluoride prophylaxis paste.

    abstract::Deciduous teeth were collected from children who received professional prophylaxis with an acidulated phosphate-fluoride (APF) or placebo paste and were analyzed to determine fluoride uptake. Enamel fluoride level was increased by the APF paste approximately 300 ppm at a depth of 2.5 micrometers during the first month...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345760550064901

    authors: Mellberg JR,Nicholson CR,Ripa LW,Barenie J

    更新日期:1976-11-01 00:00:00

  • Comparative anatomic study of mandibular growth in rats after bilateral resections of superficial masseter, posterior temporal, and anterior digastric muscles.

    abstract::Bilateral resections of the superficial masseter, posterior temporal, and anterior digastric muscles of rats were done to determine their effects on mandibular growth. The macroscopic findings support the functional matrix theory of mandibular growth. The analysis of body weight and the statistical two-way analysis of...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/00220345760550052301

    authors: Lifshitz J

    更新日期:1976-09-01 00:00:00

  • Viral gene transfer to developing mouse salivary glands.

    abstract::Branching morphogenesis is essential for the formation of salivary glands, kidneys, lungs, and many other organs during development, but the mechanisms underlying this process are not adequately understood. Microarray and other gene expression methods have been powerful approaches for identifying candidate genes that ...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/0022034511429346

    authors: Hsu JC,Di Pasquale G,Harunaga JS,Onodera T,Hoffman MP,Chiorini JA,Yamada KM

    更新日期:2012-02-01 00:00:00

  • Impact of Age on Disease Progression and Microenvironment in Oral Cancer.

    abstract::Despite the recognized link between aging and cancer, most preclinical studies in experimental tumor models are conducted with 6- to 8-wk-old rodents. The goal of the present study was to examine the impact of age on tumor incidence, growth, and microenvironmental characteristics in mouse models of head and neck squam...

    journal_title:Journal of dental research

    pub_type: 杂志文章

    doi:10.1177/0022034518775736

    authors: Vincent-Chong VK,DeJong H,Rich LJ,Patti A,Merzianu M,Hershberger PA,Seshadri M

    更新日期:2018-10-01 00:00:00