Abstract：
:During the first five rounds of cell division in the mouse embryo, spindles assemble in the absence of centrioles. Spindle formation initiates around chromosomes, but the microtubule nucleating process remains unclear. Here we demonstrate that Plk4, a protein kinase known as a master regulator of centriole formation, is also essential for spindle assembly in the absence of centrioles. Depletion of maternal Plk4 prevents nucleation and growth of microtubules and results in monopolar spindle formation. This leads to cytokinesis failure and, consequently, developmental arrest. We show that Plk4 function depends on its kinase activity and its partner protein, Cep152. Moreover, tethering Cep152 to cellular membranes sequesters Plk4 and is sufficient to trigger spindle assembly from ectopic membranous sites. Thus, the Plk4-Cep152 complex has an unexpected role in promoting microtubule nucleation in the vicinity of chromosomes to mediate bipolar spindle formation in the absence of centrioles.
journal_name
Dev Celljournal_title
Developmental cellauthors
Coelho PA,Bury L,Sharif B,Riparbelli MG,Fu J,Callaini G,Glover DM,Zernicka-Goetz Mdoi
10.1016/j.devcel.2013.09.029subject
Has Abstractpub_date
2013-12-09 00:00:00pages
586-97issue
5eissn
1534-5807issn
1878-1551pii
S1534-5807(13)00577-7journal_volume
27pub_type
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