Abstract：
:The development of multicellular organisms requires the precisely coordinated regulation of an evolutionarily conserved group of signaling pathways. Temporal and spatial control of these signaling cascades is achieved through networks of regulatory proteins, segregation of pathway components in specific subcellular compartments, or both. In vertebrates, dysregulation of primary cilia function has been strongly linked to developmental signaling defects, yet it remains unclear whether cilia sequester pathway components to regulate their activation or cilia-associated proteins directly modulate developmental signaling events. To elucidate this question, we conducted an RNAi-based screen in Drosophila non-ciliated cells to test for cilium-independent loss-of-function phenotypes of ciliary proteins in developmental signaling pathways. Our results show no effect on Hedgehog signaling. In contrast, our screen identified several cilia-associated proteins as functioning in canonical Wnt signaling. Further characterization of specific components of Intraflagellar Transport complex A uncovered a cilia-independent function in potentiating Wnt signals by promoting β-catenin/Armadillo activity.
journal_name
Dev Celljournal_title
Developmental cellauthors
Balmer S,Dussert A,Collu GM,Benitez E,Iomini C,Mlodzik Mdoi
10.1016/j.devcel.2015.07.016subject
Has Abstractpub_date
2015-09-28 00:00:00pages
705-18issue
6eissn
1534-5807issn
1878-1551pii
S1534-5807(15)00489-Xjournal_volume
34pub_type
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