Abstract:
:The regulated release of polypeptides has a central role in physiology, behavior, and development, but the mechanisms responsible for production of the large dense core vesicles (LDCVs) capable of regulated release have remained poorly understood. Recent work has implicated cytosolic adaptor protein AP-3 in the recruitment of LDCV membrane proteins that confer regulated release. However, AP-3 in mammals has been considered to function in the endolysosomal pathway and in the biosynthetic pathway only in yeast. We now find that the mammalian homolog of yeast VPS41, a member of the homotypic fusion and vacuole protein sorting (HOPS) complex that delivers biosynthetic cargo to the endocytic pathway in yeast, promotes LDCV formation through a common mechanism with AP-3, indicating a conserved role for these proteins in the biosynthetic pathway. VPS41 also self-assembles into a lattice, suggesting that it acts as a coat protein for AP-3 in formation of the regulated secretory pathway.
journal_name
Dev Celljournal_title
Developmental cellauthors
Asensio CS,Sirkis DW,Maas JW Jr,Egami K,To TL,Brodsky FM,Shu X,Cheng Y,Edwards RHdoi
10.1016/j.devcel.2013.10.007subject
Has Abstractpub_date
2013-11-25 00:00:00pages
425-37issue
4eissn
1534-5807issn
1878-1551pii
S1534-5807(13)00604-7journal_volume
27pub_type
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