Abstract：
:Optimal cell performance depends on cell size and the appropriate relative size, i.e., scaling, of the nucleus. How nuclear scaling is regulated and contributes to cell function is poorly understood, especially in skeletal muscle fibers, which are among the largest cells, containing hundreds of nuclei. Here, we present a Drosophila in vivo system to analyze nuclear scaling in whole multinucleated muscle fibers, genetically manipulate individual components, and assess muscle function. Despite precise global coordination, we find that individual nuclei within a myofiber establish different local scaling relationships by adjusting their size and synthetic activity in correlation with positional or spatial cues. While myonuclei exhibit compensatory potential, even minor changes in global nuclear size scaling correlate with reduced muscle function. Our study provides the first comprehensive approach to unraveling the intrinsic regulation of size in multinucleated muscle fibers. These insights to muscle cell biology will accelerate the development of interventions for muscle diseases.
journal_name
Dev Celljournal_title
Developmental cellauthors
Windner SE,Manhart A,Brown A,Mogilner A,Baylies MKdoi
10.1016/j.devcel.2019.02.020subject
Has Abstractpub_date
2019-04-08 00:00:00pages
48-62.e3issue
1eissn
1534-5807issn
1878-1551pii
S1534-5807(19)30143-1journal_volume
49pub_type
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