The POU domain protein spg (pou2/Oct4) is essential for endoderm formation in cooperation with the HMG domain protein casanova.

Abstract:

:The gastrulating vertebrate embryo develops three germlayers: ectoderm, mesoderm, and endoderm. Zebrafish endoderm differentiation starts with the activation of sox17 by casanova (cas). We report that spg (pou2/Oct4) is essential for endoderm formation. Embryos devoid of maternal and zygotic spg function (MZspg) lack endodermal precursors. Cell transplantations show that spg acts in early endodermal precursors, and cas mRNA-injection into MZspg embryos does not restore endoderm development. spg and cas together are both necessary and sufficient to activate endoderm development, and stimulate expression of a sox17 promoter-luciferase reporter. Endoderm and mesoderm derive from a common origin, mesendoderm. We propose that Spg and Cas commit mesendodermal precursors to an endodermal fate. The joint control of endoderm formation by spg and cas suggests that the endodermal germlayer may be a tissue unit with distinct genetic control, thus adding genetic support to the germlayer concept in metazoan development.

journal_name

Dev Cell

journal_title

Developmental cell

authors

Reim G,Mizoguchi T,Stainier DY,Kikuchi Y,Brand M

doi

10.1016/s1534-5807(03)00396-4

keywords:

subject

Has Abstract

pub_date

2004-01-01 00:00:00

pages

91-101

issue

1

eissn

1534-5807

issn

1878-1551

pii

S1534-5807(03)00396-4

journal_volume

6

pub_type

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