Abstract:
:The gastrulating vertebrate embryo develops three germlayers: ectoderm, mesoderm, and endoderm. Zebrafish endoderm differentiation starts with the activation of sox17 by casanova (cas). We report that spg (pou2/Oct4) is essential for endoderm formation. Embryos devoid of maternal and zygotic spg function (MZspg) lack endodermal precursors. Cell transplantations show that spg acts in early endodermal precursors, and cas mRNA-injection into MZspg embryos does not restore endoderm development. spg and cas together are both necessary and sufficient to activate endoderm development, and stimulate expression of a sox17 promoter-luciferase reporter. Endoderm and mesoderm derive from a common origin, mesendoderm. We propose that Spg and Cas commit mesendodermal precursors to an endodermal fate. The joint control of endoderm formation by spg and cas suggests that the endodermal germlayer may be a tissue unit with distinct genetic control, thus adding genetic support to the germlayer concept in metazoan development.
journal_name
Dev Celljournal_title
Developmental cellauthors
Reim G,Mizoguchi T,Stainier DY,Kikuchi Y,Brand Mdoi
10.1016/s1534-5807(03)00396-4keywords:
subject
Has Abstractpub_date
2004-01-01 00:00:00pages
91-101issue
1eissn
1534-5807issn
1878-1551pii
S1534-5807(03)00396-4journal_volume
6pub_type
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