Abstract：
:DNA methylation on N6-adenine (6mA) has recently been found to be a potentially epigenetic mark in several unicellular and multicellular eukaryotes. However, its distribution patterns and potential functions in land plants, which are primary producers for most ecosystems, remain largely unknown. Here we report global profiling of 6mA sites at single-nucleotide resolution in the genome of Arabidopsis thaliana at different developmental stages using single-molecule real-time sequencing. 6mA sites are widely distributed across the Arabidopsis genome and enriched over the pericentromeric heterochromatin regions. 6mA occurs more frequently in gene bodies than intergenic regions. Analysis of 6mA methylomes and RNA sequencing data demonstrates that 6mA frequency positively correlates with the gene expression level and the transition from vegetative to reproductive growth in Arabidopsis. Our results uncover 6mA as a DNA mark associated with actively expressed genes in Arabidopsis, suggesting that 6mA serves as a hitherto unknown epigenetic mark in land plants.
journal_name
Dev Celljournal_title
Developmental cellauthors
Liang Z,Shen L,Cui X,Bao S,Geng Y,Yu G,Liang F,Xie S,Lu T,Gu X,Yu Hdoi
10.1016/j.devcel.2018.03.012subject
Has Abstractpub_date
2018-05-07 00:00:00pages
406-416.e3issue
3eissn
1534-5807issn
1878-1551pii
S1534-5807(18)30232-6journal_volume
45pub_type
杂志文章abstract：:Oriented cell division plays a key role in controlling organogenesis. The mechanisms for regulating division orientation at the whole-organ level are only starting to become understood. By combining 3D time-lapse imaging, mouse genetics, and mathematical modeling, we find that global orientation of cell division is th...
journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/j.devcel.2017.12.013
更新日期：2018-02-05 00:00:00
abstract：:The appearance of Oskar protein occurs coincident with localization of oskar mRNA to the posterior pole of the Drosophila oocyte, and earlier accumulation of the protein is prevented by translational repression. We find that the nascent polypeptide-associated complex (NAC) is required for correct localization of oskar...
journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/j.devcel.2004.06.009
更新日期：2004-07-01 00:00:00
abstract：:The kinase Plk1 plays multiple roles in regulating mitotic progression, including stabilization of spindle poles, but its substrates are largely unknown. A new study by Yamamoto and coworkers has identified a centrosomal protein, Kizuna (Kiz), as a mitotic substrate of Plk1 (Oshimori et al., 2006). Phosphorylation of ...
journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/j.devcel.2006.09.012
更新日期：2006-10-01 00:00:00
abstract：:The NAD+-dependent deacetylase Sirtuin 1 (SIRT1) is down-regulated in triple-negative breast cancer. To determine the mechanistic basis by which reduced SIRT1 expression influences processes related to certain aggressive cancers, we examined the consequences of depleting breast cancer cells of SIRT1. We discovered tha...
journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/j.devcel.2019.03.011
更新日期：2019-05-06 00:00:00
abstract：:MicroRNAs are abundant in animal genomes, yet little is known about their functions in vivo. Here, we report the production of 80 new Drosophila miRNA mutants by targeted homologous recombination. These mutants remove 104 miRNAs. Together with 15 previously reported mutants, this collection includes 95 mutants deletin...
journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/j.devcel.2014.11.029
更新日期：2014-12-22 00:00:00
abstract：:Piwi family protein Aubergine (Aub) maintains genome integrity in late germ cells of the Drosophila ovary through Piwi-associated RNA-mediated repression of transposon activities. Although it is highly expressed in germline stem cells (GSCs) and early progeny, it remains unclear whether it plays any roles in early GSC...
journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/j.devcel.2017.03.023
更新日期：2017-04-24 00:00:00
abstract：:Pancreatic beta cells have been shown to be heterogeneous at multiple levels. However, spatially interrogating transcriptional heterogeneity in the intact tissue has been challenging. Here, we developed an optimized protocol for single-molecule transcript imaging in the intact pancreas and used it to identify a sub-po...
journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/j.devcel.2018.11.001
更新日期：2019-01-07 00:00:00
abstract：:Successive cell divisions during embryonic cleavage create increasingly smaller cells, so intracellular structures must adapt accordingly. Mitotic spindle size correlates with cell size, but the mechanisms for this scaling remain unclear. Using live cell imaging, we analyzed spindle scaling during embryo cleavage in t...
journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/j.devcel.2018.04.022
更新日期：2018-05-21 00:00:00
abstract：:Lipid droplets are fat storage organelles in cells that physically resemble stable oil-water emulsion droplets. In this issue of Developmental Cell, Ben M'barek et al. (2017) show that the resemblance is more than superficial: physical principles governing emulsion stability also control lipid droplet nucleation and g...
journal_title：Developmental cell
pub_type： 评论,杂志文章,评审
doi：10.1016/j.devcel.2017.06.001
更新日期：2017-06-19 00:00:00
abstract：:The biogenesis of lipid droplets (LDs) and the development of adipocytes are two key aspects of mammalian fat storage. SEIPIN, an integral membrane protein of the endoplasmic reticulum (ER), plays a critical role in both LD formation and adipogenesis. The molecular function of SEIPIN, however, has yet to be elucidated...
journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/j.devcel.2018.09.010
更新日期：2018-10-22 00:00:00
abstract：:The Drosophila lipoprotein particle, Lipophorin, bears lipid-linked morphogens on its surface and is required for long-range signaling activity of Wingless and Hedgehog. Heparan sulfate proteoglycans are also critical for trafficking and signaling of these morphogens. Here we show that Lipophorin interacts with the he...
journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/j.devcel.2007.04.019
更新日期：2007-07-01 00:00:00
abstract：:To maintain mechanical homeostasis, cells must recognize and respond to changes in cytoskeletal integrity. By imaging live cells expressing fluorescently tagged cytoskeletal proteins, we observed that actin stress fibers undergo local, acute, force-induced elongation and thinning events that compromise their stress tr...
journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/j.devcel.2010.08.008
更新日期：2010-09-14 00:00:00
abstract：:Efficient regeneration following injury is critical for maintaining tissue function and enabling organismal survival. Cells reconstituting damaged tissue are often generated from resident stem or progenitor cells or from cells that have dedifferentiated and become proliferative. While lineage-tracing studies have defi...
journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/j.devcel.2015.04.025
更新日期：2015-06-22 00:00:00
abstract：:The frizzled (fz) gene of Drosophila is required for planar polarity establishment in the adult cuticle, acting both cell autonomously and nonautonomously. We demonstrate that these two activities of fz in planar polarity are temporally separable in both the eye and wing. The nonautonomous function is dishevelled (dsh...
journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/s1534-5807(02)00363-5
更新日期：2002-12-01 00:00:00
abstract：:Constitutive exocytosis delivers newly synthesized proteins, lipids, and other molecules from the Golgi apparatus to the cell surface. This process is mediated by vesicles, which bud off the trans-Golgi network, move along cytoskeletal filaments, and fuse with the plasma membrane. Here, we show that the small GTPase R...
journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/j.devcel.2007.06.010
更新日期：2007-08-01 00:00:00
abstract：:The vertebral column derives from somites generated by segmentation of presomitic mesoderm (PSM). Somitogenesis involves a molecular oscillator, the segmentation clock, controlling periodic Notch signaling in the PSM. Here, we establish a novel link between Wnt/beta-catenin signaling and the segmentation clock. Axin2,...
journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/s1534-5807(03)00055-8
更新日期：2003-03-01 00:00:00
abstract：:The complex muscle patterns of higher organisms arise as migrating myoblasts are guided toward and connect with specific attachment sites. We review here the current understanding of myotube migration, focusing on its dynamic nature and the few molecular cues that have been identified to date. Much of this knowledge c...
journal_title：Developmental cell
pub_type： 杂志文章,评审
doi：10.1016/j.devcel.2004.06.010
更新日期：2004-07-01 00:00:00
abstract：:In this issue of Developmental Cell, Yao et al. (2019) disrupt mitochondrial respiration in limb skeletal progenitors by conditionally deleting Tfam in the mouse embryo. They discover that not only is mitochondrial respiration dispensable for embryonic cartilage development, but also its elimination obviates the need ...
journal_title：Developmental cell
pub_type： 评论,杂志文章
doi：10.1016/j.devcel.2019.05.030
更新日期：2019-06-03 00:00:00
abstract：:Chronic inflammation is associated with tumorigenesis, but how acute inflammation affects the tumor microenvironment is less known. Recently, Antonio et al. (2015) found that neutrophils attracted to an acute wound such as a biopsy drive cell proliferation of nearby pre-neoplastic cells, suggesting that acute wounds m...
journal_title：Developmental cell
pub_type： 评论,杂志文章
doi：10.1016/j.devcel.2015.07.005
更新日期：2015-07-27 00:00:00
abstract：:The adaptor complex AP-2 plays an important role in cargo selection and clathrin lattice formation during clathrin-mediated endocytosis. In a recent issue of Molecular Cell, Honing et al. demonstrate that high-affinity AP-2 membrane association is achieved through a combination of low-affinity interactions with membra...
journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/j.devcel.2005.05.013
更新日期：2005-06-01 00:00:00
abstract：:Developmental processes that control root system architecture are critical for soil exploration by plants, allowing for uptake of water and nutrients. Conversion of the auxin precursor indole-3-butyric acid (IBA) to active auxin (indole-3-acetic acid; IAA) modulates lateral root formation. However, mechanisms governin...
journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/j.devcel.2019.06.010
更新日期：2019-09-09 00:00:00
abstract：:Tethering factors have been shown to interact with Rabs and SNAREs and, more recently, with coat proteins. Coat proteins are required for cargo selection and membrane deformation to bud a transport vesicle from a donor compartment. It was once thought that a vesicle must uncoat before it recognizes its target membrane...
journal_title：Developmental cell
pub_type： 杂志文章,评审
doi：10.1016/j.devcel.2007.04.005
更新日期：2007-05-01 00:00:00
abstract：:Early leaf growth is sustained by cell proliferation and subsequent cell expansion that initiates at the leaf tip and proceeds in a basipetal direction. Using detailed kinematic and gene expression studies to map these stages during early development of the third leaf of Arabidopsis thaliana, we showed that the cell-c...
journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/j.devcel.2011.11.011
更新日期：2012-01-17 00:00:00
abstract：:Little is known about the mechanisms governing neonatal growth and maturation of organs. Here we demonstrate that calcineurin/Nuclear Factor of Activated T cells (Cn/NFAT) signaling regulates neonatal pancreatic development in mouse and human islets. Inactivation of calcineurin b1 (Cnb1) in mouse islets impaired dense...
journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/j.devcel.2012.05.014
更新日期：2012-07-17 00:00:00
abstract：:Accurate chromosome segregation in mitosis and meiosis requires that the cohesin complex be protected at the centromere by the Shugoshin/MEI-S332 protein family. Recent studies show that Sgo directly binds the phosphatase PP2A, tethering it to the centromere where it can protect cohesin subunits from phosphorylation, ...
journal_title：Developmental cell
pub_type： 评论,杂志文章
doi：10.1016/j.devcel.2006.04.017
更新日期：2006-05-01 00:00:00
abstract：:Centromere chromatin containing histone H3 variant CENP-A is required for accurate chromosome segregation as a foundation for kinetochore assembly. Human centromere chromatin assembles on a part of the long α-satellite (alphoid) DNA array, where it is flanked by pericentric heterochromatin. Heterochromatin spreads int...
journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/j.devcel.2016.05.006
更新日期：2016-06-06 00:00:00
abstract：:During organogenesis, coherent organ growth arises from spatiotemporally coordinated decisions of individual cells. In the root of Arabidopsis thaliana, this coordination results in the establishment of a division and a differentiation zone. Cells continuously move through these zones; thus, a major question is how th...
journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/j.devcel.2020.04.004
更新日期：2020-05-18 00:00:00
abstract：:Genome condensation during mitosis presents a chromatin landscape largely inaccessible to RNA polymerase II and most transcription factors. Caravaca et al. (2013) now report in Genes and Development that the pioneer transcription factor FOXA1 is retained at mitotic chromosomes, bookmarking the genome to enable gene ex...
journal_title：Developmental cell
pub_type： 评论,杂志文章
doi：10.1016/j.devcel.2013.02.005
更新日期：2013-02-25 00:00:00
abstract：:The expression of autophagy and lysosomal genes is coordinated by the transcription factor EB (TFEB). In this issue of Developmental Cell, Chen et al. identify an evolutionary conserved mode of TFEB regulation, which entails the inhibition of TFEB phase separation in the nucleus by inositol polyphosphate multikinase. ...
journal_title：Developmental cell
pub_type： 杂志文章
doi：10.1016/j.devcel.2020.11.005
更新日期：2020-12-07 00:00:00
abstract：:Stem cell differentiation involves a delicate balance of gene expression and transposon repression. In this issue of Developmental Cell, Shibata et al. (2016) show that a PIWI protein expressed in planarian stem cells is inherited by their differentiating descendants to ensure regenerative capacity of the flatworm via...
journal_title：Developmental cell
pub_type： 评论,杂志文章
doi：10.1016/j.devcel.2016.04.022
更新日期：2016-05-09 00:00:00
