Loss of Sirtuin 1 Alters the Secretome of Breast Cancer Cells by Impairing Lysosomal Integrity.

Abstract:

:The NAD+-dependent deacetylase Sirtuin 1 (SIRT1) is down-regulated in triple-negative breast cancer. To determine the mechanistic basis by which reduced SIRT1 expression influences processes related to certain aggressive cancers, we examined the consequences of depleting breast cancer cells of SIRT1. We discovered that reducing SIRT1 levels decreased the expression of one particular subunit of the vacuolar-type H+ ATPase (V-ATPase), which is responsible for proper lysosomal acidification and protein degradation. This impairment in lysosomal function caused a reduction in the number of multi-vesicular bodies (MVBs) targeted for lysosomal degradation and resulted in larger MVBs prior to their fusing with the plasma membrane to release their contents. Collectively, these findings help explain how reduced SIRT1 expression, by disrupting lysosomal function and generating a secretome comprising exosomes with unique cargo and soluble hydrolases that degrade the extracellular matrix, can promote processes that increase breast-cancer-cell survival and invasion.

journal_name

Dev Cell

journal_title

Developmental cell

authors

Latifkar A,Ling L,Hingorani A,Johansen E,Clement A,Zhang X,Hartman J,Fischbach C,Lin H,Cerione RA,Antonyak MA

doi

10.1016/j.devcel.2019.03.011

subject

Has Abstract

pub_date

2019-05-06 00:00:00

pages

393-408.e7

issue

3

eissn

1534-5807

issn

1878-1551

pii

S1534-5807(19)30188-1

journal_volume

49

pub_type

杂志文章
  • High-Molecular-Weight Hyaluronan Is a Hippo Pathway Ligand Directing Cell Density-Dependent Growth Inhibition via PAR1b.

    abstract::High-molecular-weight hyaluronan, a major component of the extracellular matrix, is anti-oncogenic, whereas low-molecular-weight hyaluronan is pro-oncogenic, though the mechanisms underlying the size-dependent opposite bioactivities of hyaluronan remain uncertain. We show here that treatment with high-molecular-weight...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2019.04.018

    authors: Ooki T,Murata-Kamiya N,Takahashi-Kanemitsu A,Wu W,Hatakeyama M

    更新日期:2019-05-20 00:00:00

  • Global analysis of the meiotic crossover landscape.

    abstract::Tight control of the number and distribution of crossovers is of great importance for meiosis. Crossovers establish chiasmata, which are physical connections between homologous chromosomes that provide the tension necessary to align chromosomes on the meiotic spindle. Understanding the mechanisms underlying crossover ...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2008.07.006

    authors: Chen SY,Tsubouchi T,Rockmill B,Sandler JS,Richards DR,Vader G,Hochwagen A,Roeder GS,Fung JC

    更新日期:2008-09-01 00:00:00

  • Cyclin-like oscillations in levels of the nucleoporin Nup96 control G1/S progression.

    abstract::In this issue of Developmental Cell, Chakraborty et al. report that efficient advancement through G1/S phase requires the downregulation of Nup96 levels during M phase. The authors show that oscillations in Nup96 levels plays a key role in maintaining normal cell proliferation, likely by regulating the export of speci...

    journal_title:Developmental cell

    pub_type: 评论,杂志文章

    doi:10.1016/j.devcel.2008.10.010

    authors: Wozniak RW,Goldfarb DS

    更新日期:2008-11-01 00:00:00

  • A Feedback Loop Controlling Organ Size.

    abstract::To maintain proper organ size, nature has devised trans-organ communication systems-involving both paracrine and circulating regulatory factors-to safeguard homeostasis. In this issue of Developmental Cell, Ji et al. (2019) now describe an enterohepatic feedback loop that balances tissue size and function in the mamma...

    journal_title:Developmental cell

    pub_type: 评论,杂志文章

    doi:10.1016/j.devcel.2019.02.008

    authors: Merrell AJ,Stanger BZ

    更新日期:2019-02-25 00:00:00

  • Actin cable dynamics and Rho/Rock orchestrate a polarized cytoskeletal architecture in the early steps of assembling a stratified epithelium.

    abstract::To enable stratification and barrier function, the epidermis must permit self-renewal while maintaining adhesive connections. By generating K14-GFP-actin mice to monitor actin dynamics in cultured primary keratinocytes, we uncovered a role for the actin cytoskeleton in establishing cellular organization. During epider...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/s1534-5807(02)00259-9

    authors: Vaezi A,Bauer C,Vasioukhin V,Fuchs E

    更新日期:2002-09-01 00:00:00

  • Cilia Control Fat Deposition during Tissue Repair.

    abstract::Fibro/adipogenic progenitors (FAPs) are emerging as crucial regulators of fibrous and fat deposits during skeletal muscle regeneration. In a recent issue of Cell, Kopinke et al. (2017) report that primary cilia induce the adipogenic fate of FAPs in injured and diseased muscle by restraining Hedgehog signaling. ...

    journal_title:Developmental cell

    pub_type: 评论,杂志文章

    doi:10.1016/j.devcel.2017.06.023

    authors: Perdiguero E,Serrano AL,Muñoz-Cánoves P

    更新日期:2017-07-24 00:00:00

  • The PAR-6 polarity protein regulates dendritic spine morphogenesis through p190 RhoGAP and the Rho GTPase.

    abstract::The majority of excitatory synaptic transmission in the brain occurs at dendritic spines, which are actin-rich protrusions on the dendrites. The asymmetric nature of these structures suggests that proteins regulating cell polarity might be involved in their formation. Indeed, the polarity protein PAR-3 is required for...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2007.11.020

    authors: Zhang H,Macara IG

    更新日期:2008-02-01 00:00:00

  • ROS Inhibits Cell Growth by Regulating 4EBP and S6K, Independent of TOR, during Development.

    abstract::Reactive oxygen species (ROS), despite having damaging roles, serve as signaling molecules regulating diverse biological and physiological processes. Employing in vivo genetic studies in Drosophila, we show that besides causing G1-S arrest by activation of Dacapo, ROS can simultaneously inhibit cell growth by regulati...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2019.04.008

    authors: Toshniwal AG,Gupta S,Mandal L,Mandal S

    更新日期:2019-05-06 00:00:00

  • Reaching a genetic and molecular understanding of skeletal development.

    abstract::In the last ten years, we have made considerable progress in our genetic and molecular understanding of all aspects of skeletal development, chondrogenesis, joint formation, and osteogenesis. This review addresses the role of the principal growth factors and transcription factors affecting these different processes an...

    journal_title:Developmental cell

    pub_type: 杂志文章,评审

    doi:10.1016/s1534-5807(02)00157-0

    authors: Karsenty G,Wagner EF

    更新日期:2002-04-01 00:00:00

  • Kinetochore-dependent microtubule rescue ensures their efficient and sustained interactions in early mitosis.

    abstract::How kinetochores regulate microtubule dynamics to ensure proper kinetochore-microtubule interactions is unknown. Here, we studied this during early mitosis in Saccharomyces cerevisiae. When a microtubule shrinks and its plus end reaches a kinetochore bound to its lateral surface, the microtubule end attempts to tether...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2011.09.006

    authors: Gandhi SR,Gierliński M,Mino A,Tanaka K,Kitamura E,Clayton L,Tanaka TU

    更新日期:2011-11-15 00:00:00

  • LHY and CCA1 are partially redundant genes required to maintain circadian rhythms in Arabidopsis.

    abstract::Several genes are known to regulate circadian rhythms in Arabidopsis, but the identity of the central oscillator has not been established. LHY and CCA1 are related MYB-like transcription factors proposed to be closely involved. Here we demonstrate that, as shown previously for CCA1, inactivation of LHY shortens the pe...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/s1534-5807(02)00170-3

    authors: Mizoguchi T,Wheatley K,Hanzawa Y,Wright L,Mizoguchi M,Song HR,Carré IA,Coupland G

    更新日期:2002-05-01 00:00:00

  • Chewing the fat; regulating autophagy in Drosophila.

    abstract::Autophagy is the major cellular process responsible for bulk cytoplasmic degradation. Two reports in this issue of Developmental Cell describe how both PI3 kinase and TOR signaling in Drosophila are critical for controlling autophagy in response to developmental and environmental cues. ...

    journal_title:Developmental cell

    pub_type: 评论,杂志文章

    doi:10.1016/j.devcel.2004.07.014

    authors: Grewal SS,Saucedo LJ

    更新日期:2004-08-01 00:00:00

  • PCYT1A Regulates Phosphatidylcholine Homeostasis from the Inner Nuclear Membrane in Response to Membrane Stored Curvature Elastic Stress.

    abstract::Cell and organelle membranes consist of a complex mixture of phospholipids (PLs) that determine their size, shape, and function. Phosphatidylcholine (PC) is the most abundant phospholipid in eukaryotic membranes, yet how cells sense and regulate its levels in vivo remains unclear. Here we show that PCYT1A, the rate-li...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2018.04.012

    authors: Haider A,Wei YC,Lim K,Barbosa AD,Liu CH,Weber U,Mlodzik M,Oras K,Collier S,Hussain MM,Dong L,Patel S,Alvarez-Guaita A,Saudek V,Jenkins BJ,Koulman A,Dymond MK,Hardie RC,Siniossoglou S,Savage DB

    更新日期:2018-05-21 00:00:00

  • Breaking Bad: Extracellular Vesicles Provoke Tumorigenic Responses Under Oxygen Deprivation.

    abstract::Intercellular communication is vital to tumor progression. In this issue of Developmental Cell, Bertolini et al. (2020) describe how small extracellular vesicles released from hypoxic mammary tumor cells facilitate intercellular communication, leading to alterations in mitochondrial dynamics and acquisition of invasiv...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2020.09.027

    authors: Boomgarden AC,Clancy JW,D'Souza-Schorey C

    更新日期:2020-10-26 00:00:00

  • The grapes of incompatibility.

    abstract::When two colonies of the tunicate Botryllus contact each other, they either fuse into a single colony or mount a destructive reaction, which keeps them apart. Which of the two reactions takes place is determined by a single, highly polymorphic fusion/histocompatibility (FuHC) locus. Recent communications report the cl...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2005.12.009

    authors: Klein J

    更新日期:2006-01-01 00:00:00

  • Wnt signals and frizzled activity orient anterior-posterior axon outgrowth in C. elegans.

    abstract::Secreted proteins of the Wnt family affect axon guidance, asymmetric cell division, and cell fate. We show here that C. elegans Wnts acting through Frizzled receptors can shape axon and dendrite trajectories by reversing the anterior-posterior polarity of neurons. In lin-44/Wnt and lin-17/Frizzled mutants, the polarit...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2006.01.013

    authors: Hilliard MA,Bargmann CI

    更新日期:2006-03-01 00:00:00

  • Memorizing Shape to Orient Cell Division.

    abstract::A century ago, Oscar Hertwig discovered that cells orient their cleavage plane orthogonal to their long axis. Reporting recently in Nature, Bosveld et al. (2016) shed light on how, showing that NuMA/Mud localization at tricellular junctions provides mitotic cells with the memory of interphase shape used to orient clea...

    journal_title:Developmental cell

    pub_type: 评论,杂志文章

    doi:10.1016/j.devcel.2016.03.005

    authors: Michel M,Dahmann C

    更新日期:2016-03-21 00:00:00

  • Stem cells on fire: inflammatory signaling in HSC emergence.

    abstract::Inflammatory pathways protect the body from infection and promote healing following injury. Recent reports demonstrate the surprising involvement of these pathways during hematopoietic stem cell emergence from the hemogenic endothelium in both zebrafish and mice. ...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2014.11.026

    authors: Veldman MB,Lin S

    更新日期:2014-12-08 00:00:00

  • gurken and the I factor retrotransposon RNAs share common localization signals and machinery.

    abstract::Drosophila gurken mRNA is localized by dynein-mediated transport to a crescent near the oocyte nucleus, thus targeting the TGFalpha signal and forming the primary embryonic axes. Here, we show that gurken and the I factor, a non-LTR retrotransposon, share a small consensus RNA stem loop of defined secondary structure,...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2005.04.012

    authors: Van De Bor V,Hartswood E,Jones C,Finnegan D,Davis I

    更新日期:2005-07-01 00:00:00

  • Drosophila exocyst components Sec5, Sec6, and Sec15 regulate DE-Cadherin trafficking from recycling endosomes to the plasma membrane.

    abstract::The E-Cadherin-catenin complex plays a critical role in epithelial cell-cell adhesion, polarization, and morphogenesis. Here, we have analyzed the mechanism of Drosophila E-Cadherin (DE-Cad) localization. Loss of function of the Drosophila exocyst components sec5, sec6, and sec15 in epithelial cells results in DE-Cad ...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2005.07.013

    authors: Langevin J,Morgan MJ,Sibarita JB,Aresta S,Murthy M,Schwarz T,Camonis J,Bellaïche Y

    更新日期:2005-09-01 00:00:00

  • Coats, tethers, Rabs, and SNAREs work together to mediate the intracellular destination of a transport vesicle.

    abstract::Tethering factors have been shown to interact with Rabs and SNAREs and, more recently, with coat proteins. Coat proteins are required for cargo selection and membrane deformation to bud a transport vesicle from a donor compartment. It was once thought that a vesicle must uncoat before it recognizes its target membrane...

    journal_title:Developmental cell

    pub_type: 杂志文章,评审

    doi:10.1016/j.devcel.2007.04.005

    authors: Cai H,Reinisch K,Ferro-Novick S

    更新日期:2007-05-01 00:00:00

  • WRAMPing up calcium in migrating cells by localized ER transport.

    abstract::Morphological plasticity and front-rear polarity are essential for directed cell migration. In this issue of Developmental Cell, Witze et al. (2013) demonstrate that Wnt5a-mediated signaling induces localization of the cortical endoplasmic reticulum to the trailing edge of melanoma cells and mediates calcium flux, rea...

    journal_title:Developmental cell

    pub_type: 评论,杂志文章

    doi:10.1016/j.devcel.2013.09.013

    authors: Mladinich KM,Huttenlocher A

    更新日期:2013-09-30 00:00:00

  • Gadd45γ and Map3k4 interactions regulate mouse testis determination via p38 MAPK-mediated control of Sry expression.

    abstract::Loss of the kinase MAP3K4 causes mouse embryonic gonadal sex reversal due to reduced expression of the testis-determining gene, Sry. However, because of widespread expression of MAP3K4, the cellular basis of this misregulation was unclear. Here, we show that mice lacking Gadd45γ also exhibit XY gonadal sex reversal ca...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2012.09.016

    authors: Warr N,Carre GA,Siggers P,Faleato JV,Brixey R,Pope M,Bogani D,Childers M,Wells S,Scudamore CL,Tedesco M,del Barco Barrantes I,Nebreda AR,Trainor PA,Greenfield A

    更新日期:2012-11-13 00:00:00

  • Regulation of stem cells by intersecting gradients of long-range niche signals.

    abstract::We have used Drosophila ovarian follicle stem cells (FSCs) to study how stem cells are regulated by external signals and draw three main conclusions. First, the spatial definition of supportive niche positions for FSCs depends on gradients of Hh and JAK-STAT pathway ligands, which emanate from opposite, distant sites....

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2012.09.010

    authors: Vied C,Reilein A,Field NS,Kalderon D

    更新日期:2012-10-16 00:00:00

  • Bud building by septin patch hole punching.

    abstract::Small GTPase Cdc42 triggers polarity establishment in budding yeast. In this issue of Developmental Cell, Okada et al. (2013) combine in silico modeling and cell biology to show that Cdc42, septins, and the exocytosis pathway are integrated in a feedback system to define and insulate the site of polarity in the membra...

    journal_title:Developmental cell

    pub_type: 评论,杂志文章

    doi:10.1016/j.devcel.2013.07.004

    authors: Caudron F,Barral Y

    更新日期:2013-07-29 00:00:00

  • The transcriptional control of trunk neural crest induction, survival, and delamination.

    abstract::Trunk neural crest cells are generated at the border between the neural plate and nonneural ectoderm, where they initiate a distinct program of gene expression, undergo an epithelial-mesenchymal transition (EMT), and delaminate from the neuroepithelium. Here, we provide evidence that members of three families of trans...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2004.12.010

    authors: Cheung M,Chaboissier MC,Mynett A,Hirst E,Schedl A,Briscoe J

    更新日期:2005-02-01 00:00:00

  • The Multidimensional Organization of Interorgan Communication Networks.

    abstract::Secreted molecules coordinate organ function. In a recent issue of Cell, Hudry et al. (2019) uncover a Drosophila testis-midgut interaction via cytokine and citrate signaling that regulates intestinal metabolism, spermatogenesis, and food intake. This impressive study is a striking example of the role of spatial organ...

    journal_title:Developmental cell

    pub_type: 评论,杂志文章

    doi:10.1016/j.devcel.2019.07.029

    authors: Droujinine IA,Perrimon N

    更新日期:2019-08-19 00:00:00

  • The utility of prions.

    abstract::Infectious, self-propagating protein aggregates (prions) as well as structurally related amyloid fibrils have traditionally been associated with neurodegenerative diseases in mammals. However, recent work in fungi indicates that prions are not simply aberrations of protein folding, but are in fact widespread, conserve...

    journal_title:Developmental cell

    pub_type: 杂志文章,评审

    doi:10.1016/s1534-5807(02)00118-1

    authors: Osherovich LZ,Weissman JS

    更新日期:2002-02-01 00:00:00

  • A molecular base for cell sorting at embryonic boundaries: contact inhibition of cadherin adhesion by ephrin/ Eph-dependent contractility.

    abstract::The mechanism responsible for subdividing the embryo into individual tissues is a fundamental, yet still poorly understood, question in developmental biology. Various general hypotheses have been proposed, involving differences in cell adhesion, contractility, or contact-mediated repulsion. However, the key parameter ...

    journal_title:Developmental cell

    pub_type: 杂志文章

    doi:10.1016/j.devcel.2013.09.004

    authors: Fagotto F,Rohani N,Touret AS,Li R

    更新日期:2013-10-14 00:00:00

  • Neutrophil Extracellular Traps: The Biology of Chromatin Externalization.

    abstract::Neutrophils are essential to the homeostatic mission of safeguarding host tissues, responding rapidly and diversely to breaches of the host's barriers to infection, and returning tissues to a sterile state. In response to specific stimuli, neutrophils extrude modified chromatin structures decorated with specific cytop...

    journal_title:Developmental cell

    pub_type: 杂志文章,评审

    doi:10.1016/j.devcel.2018.01.019

    authors: Sollberger G,Tilley DO,Zychlinsky A

    更新日期:2018-03-12 00:00:00