Abstract：
:It has recently been reported that a common side effect of translation-blocking morpholino antisense oligonucleotides is the induction of a set of innate immune response genes in Xenopus embryos and that splicing-blocking morpholinos lead to unexpected off-target mis-splicing events. Here, we present an analysis of all publicly available Xenopus RNA sequencing (RNA-seq) data in a reexamination of the effects of translation-blocking morpholinos on the innate immune response. Our analysis does not support the authors' general conclusion, which was based on a limited number of RNA-seq datasets. Moreover, the strong induction of an immune response appears to be specific to the tbxt/tbxt2 morpholinos. The more comprehensive study presented here indicates that using morpholinos for targeted gene knockdowns remains of considerable value for the rapid identification of gene function.
journal_name
Dev Celljournal_title
Developmental cellauthors
Paraiso KD,Blitz IL,Zhou JJ,Cho KWYdoi
10.1016/j.devcel.2019.04.019subject
Has Abstractpub_date
2019-05-20 00:00:00pages
643-650.e3issue
4eissn
1534-5807issn
1878-1551pii
S1534-5807(19)30316-8journal_volume
49pub_type
杂志文章,评审abstract：:Here, we show that the liver-derived apolipoprotein M (ApoM) protects the lung and kidney from pro-fibrotic insults and that this circulating factor is attenuated in aged mice. Aged mouse hepatocytes exhibit transcriptional suppression of ApoM. This leads to reduced sphingosine-1-phosphate (S1P) signaling via the S1P ...
journal_title：Developmental cell
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abstract：:Regulating cell proliferation and differentiation in CNS development requires both extraordinary complexity and precision. Neural progenitors receive graded overlapping signals from midline signaling centers, yet each makes a unique cell fate decision in a spatiotemporally restricted pattern. The Nde1-Lis1 complex reg...
journal_title：Developmental cell
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journal_title：Developmental cell
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journal_title：Developmental cell
pub_type： 评论,杂志文章
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journal_title：Developmental cell
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journal_title：Developmental cell
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journal_title：Developmental cell
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journal_title：Developmental cell
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journal_title：Developmental cell
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journal_title：Developmental cell
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